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We study by mean-field analysis and stochastic simulations chemical models for genetic toggle switches formed from pairs of genes that mutually repress each other. In order to determine the stability of the genetic switches, we make a…

Molecular Networks · Quantitative Biology 2007-05-23 Patrick B. Warren , Pieter Rein ten Wolde

Bistable biochemical switches are ubiquitous in gene regulatory networks and signal transduction pathways. Their switching dynamics, however, are difficult to study directly in experiments or conventional computer simulations, because…

Molecular Networks · Quantitative Biology 2009-11-10 Rosalind J. Allen , Patrick B. Warren , Pieter Rein ten Wolde

We study the statistical properties of a simple genetic regulatory network that provides heterogeneity within a population of cells. This network consists of a binary genetic switch in which stochastic flipping between the two switch states…

Soft Condensed Matter · Physics 2009-03-31 Paolo Visco , Rosalind J. Allen , Martin R. Evans

We present an analytical treatment of a genetic switch model consisting of two mutually inhibiting genes operating without cooperative binding of the corresponding transcription factors. Previous studies have numerically shown that these…

Molecular Networks · Quantitative Biology 2015-11-18 Tommaso Biancalani , Michael Assaf

Epigenetic modifications of histones crucially affect the eukaryotic gene activity, while the epigenetic histone state is largely determined by the binding of specific factors such as the transcription factors (TFs) to DNA. Here, the way…

Molecular Networks · Quantitative Biology 2020-10-28 Bhaswati Bhattacharyya , Jin Wang , Masaki Sasai

Genetic switch systems with mutual repression of two transcription factors are studied using deterministic methods (rate equations) and stochastic methods (the master equation and Monte Carlo simulations). These systems exhibit bistability,…

Molecular Networks · Quantitative Biology 2007-05-23 Adiel Loinger , Azi Lipshtat , Nathalie Q. Balaban , Ofer Biham

Cells use genetic switches to shift between alternate gene expression states, e.g., to adapt to new environments or to follow a developmental pathway. Here, we study the dynamics of switching in a generic-feedback on/off switch. Unlike…

Molecular Networks · Quantitative Biology 2015-05-27 Michael Assaf , Elijah Roberts , Zaida Luthey-Schulten

Evolution of gene regulation is crucial for our understanding of the phenotypic differences between species, populations and individuals. Sequence-specific binding of transcription factors to the regulatory regions on the DNA is a key…

Populations and Evolution · Quantitative Biology 2015-11-09 Murat Tuğrul , Tiago Paixão , Nicholas H. Barton , Gašper Tkačik

A toggle switch consists of two genes that mutually repress each other. This regulatory motif is active during cell differentiation and is thought to act as a memory device, being able to choose and maintain cell fate decisions. In this…

Molecular Networks · Quantitative Biology 2018-12-26 Michael K. Strasser , Fabian J. Theis , Carsten Marr

During tissue development, patterns of gene expression determine the spatial arrangement of cell types. In many cases, gradients of secreted signaling molecules - morphogens - guide this process. The continuous positional information…

Molecular Networks · Quantitative Biology 2017-02-08 Ruben Perez-Carrasco , Pilar Guerrero , James Briscoe , Karen Page

To survive environmental conditions, cells transcribe their response activities into encoded mRNA sequences in order to produce certain amounts of protein concentrations. The external conditions are mapped into the cell through the…

Biological Physics · Physics 2017-10-26 Andrés F. López-Lopera , Mauricio A. Álvarez

Transcription factors (TFs) are key regulators of gene expression. Based on the classical scenario in which the TF search process switches between one-dimensional motion along the DNA molecule and free Brownian motion in the nucleus, we…

Genomics · Quantitative Biology 2010-10-15 Godefroy Malherbe , David Holcman

Hill function is one of the widely used gene transcription regulation models. Its attribute of fitting may result in a lack of an underlying physical picture, yet the fitting parameters can provide information about biochemical reactions,…

Molecular Networks · Quantitative Biology 2024-03-05 Wenjia Shi , Yao Ma , Peilin Hu , Mi Pang , Xiaona Huang , Yiting Dang , Yuxin Xie , Danni Wu

The phage $\lambda$ infection of an \textit{E. coli} cell has become a paradigm for understanding the molecular processes involved in gene expression and cell signaling. This system provides an example of a genetic switch, as cells with…

Biological Physics · Physics 2012-04-10 Nathan Borggren

Homeostasis of protein concentrations in cells is crucial for their proper functioning, and this requires concentrations (at their steady-state levels) to be stable to fluctuations. Since gene expression is regulated by proteins such as…

Biological Physics · Physics 2020-11-10 Yipei Guo , Ariel Amir

Genetic switch systems with mutual repression of two transcription factors are studied using deterministic and stochastic methods. Numerous studies have concluded that cooperative binding is a necessary condition for the emergence of…

Molecular Networks · Quantitative Biology 2009-11-13 Azi Lipshtat , Adiel Loinger , Nathalie Q. Balaban , Ofer Biham

Gene expression in a cell entails random reaction events occurring over disparate time scales. Thus, molecular noise that often results in phenotypic and population-dynamic consequences sets a fundamental limit to biochemical signaling.…

Molecular Networks · Quantitative Biology 2009-02-19 C. -M. Ghim , E. Almaas

One goal of human genetics is to understand how the information for precise and dynamic gene expression programs is encoded in the genome. The interactions of transcription factors (TFs) with DNA regulatory elements clearly play an…

Genomics · Quantitative Biology 2014-04-15 Darren A. Cusanovich , Bryan Pavlovic , Jonathan K. Pritchard , Yoav Gilad

Recent experiments show that transcription factors (TFs) indeed use the facilitated diffusion mechanism to locate their target sequences on DNA in living bacteria cells: TFs alternate between sliding motion along DNA and relocation events…

Quantitative Methods · Quantitative Biology 2015-07-10 Maximilian Bauer , Emil S. Rasmussen , Michael A. Lomholt , Ralf Metzler

GTPases are molecular switches that regulate a wide range of cellular processes, such as organelle biogenesis, position, shape, and signal transduction. These enzymes operate by toggling between an active ("ON") guanosine triphosphate…

Subcellular Processes · Quantitative Biology 2020-09-18 Lucas M. Stolerman , Pradipta Ghosh , Padmini Rangamani
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