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A nanofiber-based optical tweezer is demonstrated. Trapping is achieved by combining attractive near-field optical gradient forces with repulsive electrostatic forces. Silica-coated Fe$_2$O$_3$ nanospheres of 300 diameter are trapped as…

Optics · Physics 2015-06-17 Jon D. Swaim , Joachim Knittel , Warwick P. Bowen

The hydrodynamic forces between Brownian spheres are determined from a measurement of the correlated thermal fluctuations in particle position using a new method, two-particle cross-correlation spectroscopy (TCS). A pair of 1.3 um diameter…

Soft Condensed Matter · Physics 2016-08-31 P. Bartlett , S. I. Henderson , S. J. Mitchell

As a key molecule of Life, Deoxyribonucleic acid (DNA) is the focus of numbers of investigations with the help of biological, chemical and physical techniques. From a physical point of view, both experimental and theoretical works have…

Soft Condensed Matter · Physics 2016-05-26 Manoel Manghi , Nicolas Destainville

We study theoretically and by means of molecular dynamics (MD) simulations the generation of mechanical force by grafted polyelectrolytes in an external electric field, which favors its adsorption on the grafting plane. The force arises in…

Soft Condensed Matter · Physics 2016-03-18 N. V. Brilliantov , Yu. A. Budkov , C. Seidel

We experimentally and theoretically consider highly condensed planar brushes made of charged polymers. Using x-ray reflectivity on polyelectrolytes which are anchored at the water-air interface, it is shown that such strongly stretched…

Traction Force Microscopy (TFM) computes the forces exerted at the surface of an elastic material by measuring induced deformations in volume. It is used to determine the pattern of the adhesion forces exerted by cells or by cellular…

Biological Physics · Physics 2018-02-09 Stanislav Makarchuk , Nicolas Beyer , Christian Gaiddon , Wilfried Grange , Pascal Hébraud

Using Langevin Dynamic simulations, we study effects of the shear force on the rupture of a double stranded DNA molecule. The model studied here contains two single diblock copolymers interacting with each other. The elastic constants of…

Soft Condensed Matter · Physics 2015-07-02 R. K. Mishra , T. Modi , D. Giri , S. Kumar

Optical tweezers equipped with position detection allow for application of piconewton-scale forces and high-temporal-resolution measurements of nanometer-scale motion. While typically used for trapping microscopic objects, the optical…

We studied the unzipping kinetics of single molecules of double-stranded DNA by pulling one of their two strands through a narrow protein pore. PCR analysis yielded the first direct proof of DNA unzipping in such a system. The time to unzip…

Soft Condensed Matter · Physics 2009-11-07 Alexis F. Sauer-Budge , Jacqueline A. Nyamwanda , David K. Lubensky , Daniel Branton

A purely electrical sensing scheme is presented that determines the concentration of macromolecules in solution by measuring the capacitance between planar microelectrodes. Concentrations of DNA in the ng/mL range have been used in samples…

Biological Physics · Physics 2009-01-20 Anja Henning , Joerg Henkel , Frank F Bier , Ralph Hoelzel

Optical tweezers are highly versatile laser traps for neutral microparticles, with fundamental applications in physics and in single molecule cell biology. Force measurements are performed by converting the stiffness response to…

Optics · Physics 2014-06-30 R S Dutra , N B Viana , P A Maia Neto , H M Nussenzveig

Optical tweezers find applications in various fields, ranging from biology to physics. One of the fundamental steps necessary to perform quantitative measurements using trapped particles is the calibration of the tweezer's spring constant.…

Quantum Physics · Physics 2020-06-05 Bruno Melo , Felipe Almeida , Guilherme Temporão , Thiago Guerreiro

Optical tweezers, with their high precision, dynamic control, and non-invasiveness, are increasingly important in scientific research and applications at the micro and nano scales. However, manipulation by optical tweezers is challenged by…

A simple model of DNA based on two interacting polymers has been used to study the unzipping of a double stranded DNA subjected to a periodic force. We propose a dynamical transition, where without changing the physiological condition, it…

Biological Physics · Physics 2015-06-11 Sanjay Kumar , Garima Mishra

With the continued improvement of sequencing technologies, the prospect of genome-based medicine is now at the forefront of scientific research. To realize this potential, however, we need a revolutionary sequencing method for the…

Biological Physics · Physics 2008-01-03 Michael Zwolak , Massimiliano Di Ventra

In this work, the force due to radiation pressure is measured with sub-10 pN sensitivity, corresponding to less than 2 mW of optical power. The apparatus adds homemade reflectors to a commercial Cavendish balance, which consists of a…

Physics Education · Physics 2026-01-22 Leland Russell , Ezekiel A. Rein , Anatalya Piatigorsky , Jennifer T. Heath

The mechanical properties of aggregated colloids depend on the mutual interplay of inter-particle potentials, contact forces, aggregate structure and material properties of the bare particles. Owing to this variety of influences…

Soft Condensed Matter · Physics 2012-05-15 Marcel Roth , Carsten Schilde , Philipp Lellig , Arno Kwade , Günter K. Auernhammer

The article demonstrates a technique for fabricating a structure with the inclusion of suspended DNA threads and manipulating them using composite nanotweezers with shape memory effect. This technique could be suitable for stretching of…

By exerting mechanical force it is possible to unfold/refold RNA molecules one at a time. In a small range of forces, an RNA molecule can hop between the folded and the unfolded state with force-dependent kinetic rates. Here, we introduce a…

Biological Physics · Physics 2012-08-27 M. Manosas , J. -D. Wen , P. T. X. Li , S. B. Smith , C. Bustamante , I. Tinoco, , F. Ritort

We introduce a coarse-grained rigid nucleotide model of DNA that reproduces the basic thermodynamics of short strands: duplex hybridization, single-stranded stacking and hairpin formation, and also captures the essential structural…

Soft Condensed Matter · Physics 2010-05-14 Thomas E. Ouldridge , Ard A. Louis , Jonathan P. K. Doye