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Rebinding of dissociated ligands from cell surface proteins can confound quantitative measurements of dissociation rates important for characterizing the affinity of binding interactions. This can be true also for in vitro techniques such…

Quantitative Methods · Quantitative Biology 2007-05-23 Manoj Gopalakrishnan , Kimberly Forsten-Williams , Theressa R. Cassino , Luz Padro , Thomas E. Ryan , Uwe C. Tauber

Receptor-ligand binding is a critical first step in signal transduction and the duration of the interaction can impact signal generation. In mammalian cells, clustering of receptors may be facilitated by heterogeneous zones of lipids, known…

Subcellular Processes · Quantitative Biology 2009-11-10 Manoj Gopalakrishnan , Kimberly Forsten-Williams , Matthew A. Nugent , Uwe C. Tauber

Rebinding kinetics of molecular ligands plays a critical role in biomachinery, from regulatory networks to protein transcription, and is also a key factor for designing drugs and high-precision biosensors.In this study, we investigate…

Biological Physics · Physics 2018-07-12 Aykut Erbas , Monica Olvera de la Cruz , John F. Marko

Surface plasmon resonance (SPR) chips are widely used to measure association and dissociation rates for the binding kinetics between two species of chemicals, e.g., cell receptors and ligands. It is commonly assumed that ligands are…

Quantitative Methods · Quantitative Biology 2016-12-07 Jacob Carroll , Matthew Raum , Kimberly Forsten-Williams , Uwe C. Täuber

We extend the Bell forced dissociation rate model to take account into dynamic disorder. The motivation of the present work is from the recent forced dissociation experiments of the adhesive receptor-ligand complexes, in which some…

Soft Condensed Matter · Physics 2007-05-23 Fei Liu , Zhong-can Ou-Yang

A quantitative understanding of how cells interact with their extracellular matrix via molecular bonds is fundamental for many important processes in cell biology and engineering. In these interactions, the deformability of cells and matrix…

Biological Physics · Physics 2022-07-13 Qiangzeng Huang , Jizeng Wang

A quantitative model of the mobility of functionalized particles at the interface is pivotal to understanding important systems in biology and nanotechnology. In this work, we investigate the emerging dynamics of particles anchored through…

Soft Condensed Matter · Physics 2024-06-19 Janna Lowensohn , Laurie Stevens , Daniel Goldstein , Bortolo Matteo Mognetti

Single receptor-ligand bonds have finite lifetimes, so that biological systems can dynamically react to changes in their environment. In cell adhesion, adhesion bonds usually act cooperatively in adhesion clusters. Outside the cellular…

Soft Condensed Matter · Physics 2007-05-23 T. Erdmann , U. S. Schwarz

We devise a method for predicting certain receptor-ligand binding behaviors, based on stochastic dynamical modelling. We consider the dynamics of a receptor binding to a ligand on the cell membrane, where the receptor and ligand perform…

Molecular Networks · Quantitative Biology 2023-02-17 Xi Chen , Hui Wang , Jinqiao Duan

Recently experiments showed that some biological noncovalent bonds increase their lifetimes when they are stretched by an external force, and their lifetimes will decrease when the force increases further. Several specific quantitative…

Statistical Mechanics · Physics 2009-11-11 Fei Liu , Zhong-can Ou-Yang , Mitsumasa Iwamoto

Biological sensory systems generally operate out of equilibrium, which often leads to their improved performance. Here, we study the sensitivity of ligand concentration for a general receptor model, which is generally in the non-equilibrium…

Biological Physics · Physics 2017-06-27 Takashi Okada

We propose a model of mass-conserving heterogeneous nucleation to describe the dynamics of ligand-receptor binding in closed cellular compartments. When the ligand dissociation rate is small, competition among receptors for free ligands…

Soft Condensed Matter · Physics 2015-05-30 T. Chou , M. D'Orsogna

Analysis of bond rupture data from single-molecule force spectroscopy experiments commonly relies on the strong assumption that the bond dissociation process is irreversible. However, with increased spatiotemporal resolution of instruments…

Biological Physics · Physics 2022-05-13 Jakob Tómas Bullerjahn , Gerhard Hummer

Cells use surface receptors to estimate the concentration of external ligands. Limits on the accuracy of such estimations have been well studied for pairs of ligand and receptor species. However, the environment typically contains many…

Molecular Networks · Quantitative Biology 2015-06-02 Vijay Singh , Ilya Nemenman

Cells adapt to changing environments by sensing ligand concentrations using specific receptors. The accuracy of sensing is ultimately limited by the finite number of ligand molecules bound by receptors. Previously derived physical limits to…

Subcellular Processes · Quantitative Biology 2019-11-13 Thierry Mora , Ilya Nemenman

Many types of cells can sense external ligand concentrations with cell-surface receptors at extremely high accuracy. Interestingly, ligand-bound receptors are often internalized, a process also known as receptor-mediated endocytosis. While…

Biological Physics · Physics 2015-05-18 Gerardo Aquino , Robert G. Endres

When spin relaxation is governed by spontaneous emission of a photon into the resonator used for signal detection (the Purcell effect), the relaxation time $T_1$ depends on the spin-resonator frequency detuning $\delta$ and coupling…

From nutrient uptake, to chemoreception, to synaptic transmission, many systems in cell biology depend on molecules diffusing and binding to membrane receptors. Mathematical analysis of such systems often neglects the fact that receptors…

Quantitative Methods · Quantitative Biology 2021-06-16 Gregory Handy , Sean D Lawley

Explicit analytical expressions for the drag and diffusion coefficients of a spherical particle attached to the interface between two immiscible fluids are constructed for the case of a small viscosity ratio between the fluid phases. The…

Soft Condensed Matter · Physics 2016-04-20 Aaron Dörr , Steffen Hardt

We study diffusion of particles on the surface of a sphere toward a partially reactive circular target with partly reversible binding kinetics. We solve the coupled diffusion-reaction equations and obtain the exact expressions for the…

Statistical Mechanics · Physics 2019-11-05 Denis S. Grebenkov
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