Structural correlations and melting of B-DNA fibres
Abstract
Despite numerous attempts, the understanding of the thermal denaturation of DNA is still a challenge due to the lack of structural data at the transition since standard experimental approaches to DNA melting are made in solution and do not provide spatial information. We report a measurement using neutron scattering from oriented DNA fibres to determine the size of the regions that stay in the double-helix conformation as the melting temperature is approached from below. A Bragg peak from the B-form of DNA has been observed as a function of temperature and its width and integrated intensity have bean measured. These results, complemented by a differential calorimetry study of the melting of B DNA fibres as well as electrophoresis and optical observation data, are analysed in terms of a one-dimensional mesoscopic model of DNA.
Keywords
Cite
@article{arxiv.1106.2632,
title = {Structural correlations and melting of B-DNA fibres},
author = {Andrew Wildes and Nikos Theodorakopoulos and Jessica Valle Orero and Santiago Cuesta-Lopez and Jean-Luc Garden and Michel Peyrard},
journal= {arXiv preprint arXiv:1106.2632},
year = {2011}
}