English

Shot noise limited high speed stimulated Raman microscopy

Optics 2019-05-29 v1 Biological Physics

Abstract

We report a shot noise limited high speed stimulated Raman microscopy platform allowing to acquire molecular vibrational spectra over \SI{200}{\per \cm} in \SI{12}{\micro \second} at a scan rate of 40kHz. Using spectral focusing together with optimized acousto-optics programmable dispersive filters the designed low noise imaging platform performs chemical imaging of dynamical processes such as Mannitol crystal hydratation and reaches a signal to noise ratio sufficient to perform label free histological imaging on frozen human colon tissue slides.

Keywords

Cite

@article{arxiv.1905.11953,
  title  = {Shot noise limited high speed stimulated Raman microscopy},
  author = {Xavier Audier and Nicolas Forget and Hervé Rigneault},
  journal= {arXiv preprint arXiv:1905.11953},
  year   = {2019}
}