Osmotic stress affects functional properties of human melanoma cell lines
Abstract
Understanding the role of microenvironment in cancer growth and metastasis is a key issue for cancer research. Here, we study the effect of osmotic pressure on the functional properties of primary and metastatic melanoma cell lines. In particular, we experimentally quantify individual cell motility and transmigration capability. We then perform a circular scratch assay to study how a cancer cell front invades an empty space. Our results show that primary melanoma cells are sensitive to a low osmotic pressure, while metastatic cells are less. To better understand the experimental results, we introduce and study a continuous model for the dynamics of a cell layer and a stochastic discrete model for cell proliferation and diffusion. The two models capture essential features of the experimental results and allow to make predictions for a wide range of experimentally measurable parameters.
Keywords
Cite
@article{arxiv.1504.00888,
title = {Osmotic stress affects functional properties of human melanoma cell lines},
author = {Caterina A. M. La Porta and Anna Ghilardi and Maria Pasini and Lasse Laurson and Mikko J. Alava and Stefano Zapperi and Martine Ben Amar},
journal= {arXiv preprint arXiv:1504.00888},
year = {2015}
}