High fidelity fibre-based physiological sensing deep in tissue
Abstract
Physiological sensing deep in tissue, remains a clinical challenge. Here a flexible miniaturised sensing optrode providing a platform to perform minimally invasive in vivo in situ measurements is reported. Silica microspheres covalently coupled with a high density of ratiometrically configured fluorophores were deposited into etched pits on the distal end of a 150 micron diameter multicore optical fibre. With this platform, multiplexed photonic measurements of pH and oxygen concentration with high precision in the distal alveolar space of the lung is reported. We demonstrated the phenomenon that high-density deposition of carboxyfluorescein covalently coupled to silica microspheres shows an inverse shift in fluorescence in response to varying pH. This platform delivered fast and accurate measurements, near instantaneous response time, no photobleaching, immunity to power fluctuations and a flexible architecture for addition of multiple sensors.
Cite
@article{arxiv.1808.08883,
title = {High fidelity fibre-based physiological sensing deep in tissue},
author = {Tushar R. Choudhary and Michael G. Tanner and Alicia Megia-Fernandez and Kerrianne Harrington and Harry A. Wood and Adam Marshall and Patricia Zhu and Sunay V. Chankeshwara and Debaditya Choudhury and Graham Monro and Muhammed Ucuncu and Fei Yu and Rory R. Duncan and Robert R. Thomson and Kevin Dhaliwal and Mark Bradley},
journal= {arXiv preprint arXiv:1808.08883},
year = {2018}
}