With the introduction of cyber-physical genome sequencing and editing technologies, such as CRISPR, researchers can more easily access tools to investigate and create remedies for a variety of topics in genetics and health science (e.g. agriculture and medicine). As the field advances and grows, new concerns present themselves in the ability to predict the off-target behavior. In this work, we explore the underlying biological and chemical model from a data driven perspective. Additionally, we present a machine learning based solution named \textit{Guide-Guard} to predict the behavior of the system given a gRNA in the CRISPR gene-editing process with 84\% accuracy. This solution is able to be trained on multiple different genes at the same time while retaining accuracy.
@article{arxiv.2602.16327,
title = {Guide-Guard: Off-Target Predicting in CRISPR Applications},
author = {Joseph Bingham and Netanel Arussy and Saman Zonouz},
journal= {arXiv preprint arXiv:2602.16327},
year = {2026}
}