We have measured the fluorescence spectrum for fluorescein solution in ethanol with concentration 1 {\times} 10-3 mol/liter at different temperatures from room temperature to freezing point of solvent, (T = 153, 183, 223, 253, and 303 K) using liquid nitrogen. Table curve 2D version 5.01 program has been used to determine the fitting curve and fitting equation for each fluorescence spectrum. Fourier series (3 {\times} 2) was the most suitable fitting equation for all spectra. Theoretical fluorescence spectrum of fluorescein in ethanol at T = 183K was calculated and compared with experimental fluorescence spectrum at the same temperature. There is a good similarity between them.
Cite
@article{arxiv.1109.1458,
title = {Fluorescence spectrum analysis using Fourier series modeling for Fluorescein solution in Ethanol},
author = {Mahasin F. Hadi},
journal= {arXiv preprint arXiv:1109.1458},
year = {2011}
}