Counting and differentiating aquatic biotic nanoparticles by full-field interferometry: from laboratory tests to Tara Oceans sample analysis
Medical Physics
2016-07-05 v1 Biological Physics
Abstract
There is a huge abundance of viruses and membrane vesicles in seawater. We describe a new full-field, incoherently illuminated, shot-noise limited, common-path interferometric detection method that we couple with the analysis of Brownian motion to detect, quantify, and differentiate biotic nanoparticles. We validated the method with calibrated nanoparticles and homogeneous DNA or RNA.viruses. The smallest virus size that we characterized with a suitable signal-to-noise ratio was around 30 nm in diameter. Analysis of Brownian motions revealed anisotropic trajectories for myoviruses.We further applied the method for vesicles detection and for analysis of coastal and oligotrophic samples from Tara Oceans circumnavigation.
Keywords
Cite
@article{arxiv.1607.00425,
title = {Counting and differentiating aquatic biotic nanoparticles by full-field interferometry: from laboratory tests to Tara Oceans sample analysis},
author = {Martine Boccara and Yasmina Fedala and Catherine Venien Bryan and Marc Bailly-Bechet and Chris Bowler and Albert Claude Boccara},
journal= {arXiv preprint arXiv:1607.00425},
year = {2016}
}
Comments
11 pages 4 figures