Tailored Speckle Illumination Microscopy with Enhanced Sectioning and Image Quality
Abstract
Optical speckle patterns have been widely used for illumination in computational imaging, optical sectioning microscopy, and super-resolution imaging. However, commonly used speckles satisfy Rayleigh statistics, which are not ideal for diverse imaging applications. Here we tailor three-dimensional speckle intensity statistics for dynamic speckle illumination microscopy based on linear fluorescence. Optical sectioning is enhanced by axially varying speckle contrast, and image reconstruction noise is minimized with in-focus speckles of binary intensities. The customized speckle statistics are shown to tolerate sample-induced aberration and scattering. We apply tailored speckle illumination to mouse brain vascular imaging and demonstrate much improved image quality than optical-sectioning structured illumination. These results establish customization of speckle intensity statistics as a promising strategy for robust, high-throughput fluorescence imaging in thick, scattering biological specimens.
Cite
@article{arxiv.2604.20112,
title = {Tailored Speckle Illumination Microscopy with Enhanced Sectioning and Image Quality},
author = {SeungYun Han and KyeoReh Lee and Young Seo Kim and Chuan Li and Nicholas Bender and Kabish Wisal and Taeyun Ku and Jerome Mertz and Hui Cao},
journal= {arXiv preprint arXiv:2604.20112},
year = {2026}
}
Comments
10 pages, 4 figures in the main text and 9 pages, 6 figures in the supplementary