Phase gradient microscopy in thick tissue with oblique back-illumination
Optics
2015-03-13 v1
Abstract
Phase contrast techniques, such as differential interference contrast (DIC) microscopy, are widely used to provide morphological images of unstained biological samples. The trans-illumination geometry required for these techniques has restricted their application to thin samples. We introduce oblique back-illumination microscopy (OBM), a method of collecting en face phase gradient images of thick scattering samples, enabling near video-rate in vivo phase imaging with a miniaturized probe suitable for endoscopy.
Cite
@article{arxiv.1206.3342,
title = {Phase gradient microscopy in thick tissue with oblique back-illumination},
author = {Tim N. Ford and Kengyeh K. Chu and Jerome Mertz},
journal= {arXiv preprint arXiv:1206.3342},
year = {2015}
}
Comments
14 pages, 3 figures, 2 supplementary figures, 7 supplementary videos