Interaction of proteins in solution from small angle scattering: a perturbative approach

In this work, an improved methodology for studying interactions of proteins in solution by small-angle scattering, is presented. Unlike the most common approach, where the protein-protein correlation functions $g_{ij}(r)$ are approximated by their zero-density limit (i.e. the Boltzmann factor), we propose a more accurate representation of $g_{ij}(r)$ which takes into account terms up to the first order in the density expansion of the mean-force potential. This improvement is expected to be particulary effective in the case of strong protein-protein interactions at intermediate concentrations. The method is applied to analyse small angle X-ray scattering data obtained as a function of the ionic strength (from 7 to 507 mM) from acidic solutions of $\beta$-Lactoglobuline at the fixed concentration of 10 $\rm g L^{-1}$. The results are compared with those obtained using the zero-density approximation and show a significant improvement particularly in the more demanding case of low ionic strength.