The success of therapeutic antibodies relies on their ability to selectively bind antigens. AI-based antibody design protocols have shown promise in generating epitope-specific designs. Many of these protocols use an inverse folding step to generate diverse sequences given a backbone structure. Due to prohibitive screening costs, it is key to identify candidate sequences likely to bind in vitro. Here, we compare the efficacy of 8 common scoring paradigms based on open-source models to classify antibody designs as binders or non-binders. We evaluate these approaches on a novel surface plasmon resonance (SPR) dataset, spanning 5 antigens. Our results show that existing methods struggle to detect binders, and performance is highly variable across antigens. We find that metrics computed on flexibly docked antibody-antigen complexes are more robust, and ensembles scores are more consistent than individual metrics. We provide experimental insight to analyze current scoring techniques, highlighting that the development of robust, zero-shot filters is an important research gap.
@article{arxiv.2312.05273,
title = {Evaluating Zero-Shot Scoring for In Vitro Antibody Binding Prediction with Experimental Validation},
author = {Divya Nori and Simon V. Mathis and Amir Shanehsazzadeh},
journal= {arXiv preprint arXiv:2312.05273},
year = {2023}
}