Deep penetration fluorescence imaging through dense yeast cells suspensions using Airy beams
Optics
2019-05-01 v1 Biological Physics
Abstract
We propose a new method to image fluorescent objects through turbid media base on Airy beam scanning. This is achieved by using the non-diffractive nature of Airy beams, namely their ability to maintain their shape while penetrating through a highly scattering medium. We show, that our technique can image fluorescent objects immersed in turbid media with higher resolution and signal to noise than confocal imaging. As proof-of-principle, we demonstrate imaging of 1m sized fluorescent beads through a dense suspension of yeast cells with an attenuation coefficient of 51cm at a depth of 90m. Finally, we demonstrate that our technique can also provide the depth of the imaged object without any additional sectioning.
Cite
@article{arxiv.1902.09010,
title = {Deep penetration fluorescence imaging through dense yeast cells suspensions using Airy beams},
author = {Harel Nagar and Yael Roichman},
journal= {arXiv preprint arXiv:1902.09010},
year = {2019}
}
Comments
5 pages, 5 figures