Biomolecular resource utilization in elementary cell-free gene circuits
Molecular Networks
2013-07-02 v1 Biomolecules
Abstract
We present a detailed dynamical model of the behavior of transcription-translation circuits in vitro that makes explicit the roles played by essential molecular resources. A set of simple two-gene test circuits operating in a cell-free biochemical 'breadboard' validate this model and highlight the consequences of limited resource availability. In particular, we are able to confirm the existence of biomolecular 'crosstalk' and isolate its individual sources. The implications of crosstalk for biomolecular circuit design and function are discussed.
Cite
@article{arxiv.1307.0178,
title = {Biomolecular resource utilization in elementary cell-free gene circuits},
author = {Dan Siegal-Gaskins and Vincent Noireaux and Richard M. Murray},
journal= {arXiv preprint arXiv:1307.0178},
year = {2013}
}
Comments
Accepted to the 2013 American Control Conference