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Phenotypic variation is a hallmark of cellular physiology. Metabolic heterogeneity, in particular, underpins single-cell phenomena such as microbial drug tolerance and growth variability. Much research has focussed on transcriptomic and…

Molecular Networks · Quantitative Biology 2019-01-31 Mona K. Tonn , Philipp Thomas , Mauricio Barahona , Diego A Oyarzún

Cell fate determination, the process through which cells commit to differentiated states is commonly mediated by gene regulatory motifs with mutually exclusive expression states. The classical deterministic picture for cell fate…

Molecular Networks · Quantitative Biology 2019-10-09 Manuel Pájaro , Irene Otero-Muras , Carlos Vázquez , Antonio A. Alonso

Heterogeneity in gene expression across isogenic cell populations can give rise to phenotypic diversity, even when cells are in homogenous environments. This diversity arises from the discrete, stochastic nature of biochemical reactions,…

Quantitative Methods · Quantitative Biology 2017-08-31 Zachary Fox , Brian Munsky

Timing is essential for many cellular processes, from cellular responses to external stimuli to the cell cycle and circadian clocks. Many of these processes are based on gene expression. For example, an activated gene may be required to…

Molecular Networks · Quantitative Biology 2017-02-24 Alma Dal Co , Marco Cosentino Lagomarsino , Michele Caselle , Matteo Osella

Using an analytically solvable stochastic model, we study the properties of a simple genetic circuit consisting of multiple copies of an self-regulating gene. We analyse how the variation in gene copy number and the mutations changing the…

Molecular Networks · Quantitative Biology 2016-08-17 Jakub Jędrak , Anna Ochab-Marcinek

Living cells must control the reading out or "expression" of information encoded in their genomes, and this regulation often is mediated by transcription factors--proteins that bind to DNA and either enhance or repress the expression of…

Molecular Networks · Quantitative Biology 2013-08-01 Gašper Tkačik , Aleksandra M Walczak , William Bialek

Stochastic feedback systems give rise to a variety of notions of stability. The conditions for the stability of the median, mean, and variance stability conditions differ. These conditions can be stated explicitly for scalar discrete-time…

Systems and Control · Electrical Eng. & Systems 2019-12-19 Roy S. Smith , Bassam Bamieh

Modeling stochasticity in gene regulatory networks is an important and complex problem in molecular systems biology. To elucidate intrinsic noise, several modeling strategies such as the Gillespie algorithm have been used successfully. This…

Molecular Networks · Quantitative Biology 2013-01-18 David Murrugarra , Alan Veliz-Cuba , Boris Aguilar , Seda Arat , Reinhard Laubenbacher

We show how to construct a reduced description of interacting genes in noisy, small regulatory networks using coupled binary "spin" variables. Treating both the protein number and gene expression state variables stochastically and on equal…

Molecular Networks · Quantitative Biology 2015-05-13 Aleksandra M. Walczak , Peter G. Wolynes

Interactions between genes and gene products give rise to complex circuits that enable cells to process information and respond to external signals. Theoretical studies often describe these interactions using continuous, stochastic, or…

Molecular Networks · Quantitative Biology 2007-05-23 Madalena Chaves , Reka Albert , Eduardo D. Sontag

Feedback loops are essential for regulating cell proliferation and maintaining the delicate balance between cell division and cell death. Thanks to the exact solution of a few simple models of cell growth it is by now clear that stochastic…

Populations and Evolution · Quantitative Biology 2023-03-07 Antonio Francesco Zirattu , Marta Biondo , Matteo Osella , Michele Caselle

The origin of stochastic fluctuations in gene expression has received considerable attention recently. Fluctuations in gene expression are particularly pronounced in cellular systems because of the small copy number of species undergoing…

Subcellular Processes · Quantitative Biology 2009-08-02 P. S. Gutierrez , D. Monteoliva , L. Diambra

Cell growth and gene expression, essential elements of all living systems, have long been the focus of biophysical interrogation. Advances in single-cell methods have invigorated theoretical studies into these processes. However, until…

Subcellular Processes · Quantitative Biology 2023-11-22 Ido Golding , Ariel Amir

We study the interplay between noise and a positive feedback mechanism in an excitable system that generates events. We show that such a system can exhibit a bistability in the dynamics of the event generation (states of low and high…

Statistical Mechanics · Physics 2014-10-22 Justus A. Kromer , Reynaldo D. Pinto , Benjamin Lindner , Lutz Schimansky-Geier

Characterization of pluripotent states, in which cells can both self-renew and differentiate, and the irreversible loss of pluripotency are important research areas in developmental biology. In particular, an understanding of these…

Molecular Networks · Quantitative Biology 2015-09-02 Tadashi Miyamoto , Chikara Furusawa , Kunihiko Kaneko

Studies on the role of fluctuations in signal propagation and on gene regulation in monoclonal bacterial population have been extensively pursued based on the machinery of two-component system. The bacterial two-component system shows noise…

Molecular Networks · Quantitative Biology 2017-01-10 Tarunendu Mapder , Sudip Chattopadhyay , Suman K Banik

Gene expression and its regulation is a nonequilibrium stochastic process. Different molecules are involved in several biochemical steps in this process with low copies. It is observed that the stochasticity in biochemical processes is…

Molecular Networks · Quantitative Biology 2019-05-22 Rajesh Karmakar

Prokaryotic gene expression is dynamic and noisy, and can lead to phenotypic variations. Previous work has shown that the selection of such variation can be modeled through basic reaction networks described by O.D.E. displaying a bistable…

Molecular Networks · Quantitative Biology 2023-04-10 Xavier Richard , Benoît Richard , Christian Mazza , Jan Roelof van der Meer

A wealth of new research has highlighted the critical roles of small RNAs (sRNAs) in diverse processes such as quorum sensing and cellular responses to stress. The pathways controlling these processes often have a central motif comprising…

Molecular Networks · Quantitative Biology 2015-05-27 Charles Baker , Tao Jia , Rahul V. Kulkarni

In multicellular organisms, several cell states coexist. For determining each cell type, cell-cell interactions are often essential, in addition to intracellular gene expression dynamics. Based on dynamical systems theory, we propose a…

Cell Behavior · Quantitative Biology 2007-12-05 Akihiko Nakajima , Kunihiko Kaneko