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There is compelling evidence that substrate stiffness affects cell adhesion as well as cytoskeleton organization and contractile activity. This work was designed to study the cytoskeletal contractile activity of cells plated on microposts…

Cell Behavior · Quantitative Biology 2019-09-13 Ian Manifacier , Kevin Beussman , Sangyoon Han , Nathan Sniadecki , Imad About , Jean-Louis Milan

The cytoskeleton is a model active matter system that controls diverse cellular processes from division to motility. While both active actomyosin dynamics and actin-microtubule interactions are key to the cytoskeleton's versatility and…

We demonstrate that a semiflexible bundle of wormlike chains exhibits a state-dependent bending stiffness that alters fundamentally its scaling behavior with respect to the standard wormlike chain. We explore the equilibrium conformational…

Statistical Mechanics · Physics 2007-07-26 Claus Heussinger , Mark Bathe , Erwin Frey

The actin cytoskeleton of adherent tissue cells often condenses into filament bundles contracted by myosin motors, so-called stress fibers, which play a crucial role in the mechanical interaction of cells with their environment. Stress…

Subcellular Processes · Quantitative Biology 2015-05-27 Achim Besser , Julien Colombelli , Ernst H. K. Stelzer , Ulrich S. Schwarz

Cell mechanical functions like locomotion, contraction and division are controlled by the cytoskeleton, a dynamic biopolymer network whose mechanical properties remain poorly understood. We perform single-cell uniaxial stretching…

Biological Physics · Physics 2007-05-23 Pablo Fernandez , Pramod A. Pullarkat , Albrecht Ott

We develop a model for gels and entangled solutions of semiflexible biopolymers such as F-actin. Such networks play a crucial structural role in the cytoskeleton of cells. We show that the rheologic properties of these networks can result…

Condensed Matter · Physics 2009-10-28 F. C. MacKintosh , J. Kas , P. A. Janmey

Actin is a key component of the cytoskeleton, which plays central roles in cell motility, division, growth, and tensile strength. To enable this wide range of transient mechanical processes and properties, networks of actin filaments…

The actin and microtubule cytoskeletons are vital structures for cell growth and development across all species. While individual molecular mechanisms underpinning actin and microtubule dynamics have been intensively studied, principles…

Semiflexible polymers such as filamentous actin play a vital role in the mechanical behavior of cells, yet the basic properties of cross-linked F-actin networks remain poorly understood. To address this issue, we have performed numerical…

Soft Condensed Matter · Physics 2009-11-10 D. A. Head , A. J. Levine , F. C. MacKintosh

The macromechanical properties of purely bundled in vitro actin networks are not only determined by the micromechanical properties of individual bundles but also by molecular unbinding events of the actin binding protein (ABP) fascin. Under…

Soft Condensed Matter · Physics 2009-11-13 Oliver Lieleg , Andreas R. Bausch

The mechanical properties of cells are dominated by the cytoskeleton, an interconnected network of long elastic filaments. The connections between the filaments are provided by crosslinking proteins, which constitute, next to the filaments,…

Soft Condensed Matter · Physics 2012-10-09 Claus Heussinger

A phenomenological description for active bundles of polar filaments is presented. The activity of the bundle results from crosslinks, that induce relative displacements between the aligned filaments. Our generic description is based on…

Statistical Mechanics · Physics 2009-11-07 Karsten Kruse , Frank Julicher

Cell migration is a fundamental process for life and is highly dependent on the dynamical and mechanical properties of the cytoskeleton. Intensive physical and biochemical crosstalk between actin, microtubules, and intermediate filaments…

Biological Physics · Physics 2024-01-17 James P. Conboy , Irene Istúriz Petitjean , Anouk van der Net , Gijsje H. Koenderink

Cytoskeletal networks, which are essentially motor-filament assemblies, play a major role in many developmental processes involving structural remodeling and shape changes. These are achieved by nonequilibrium self-organization processes…

Soft Condensed Matter · Physics 2014-12-22 Shenshen Wang , Peter G. Wolynes

Bundles of stiff filaments are ubiquitous in the living world, found both in the cytoskeleton and in the extracellular medium. These bundles are typically held together by smaller cross-linking molecules. We demonstrate analytically,…

The mechanical characteristics of fibers (of various materials), as well as of fiber bundles, are of primary importance for the design and the mechanical behavior of textiles, or of fibrous and composite materials. These characteristics are…

Soft Condensed Matter · Physics 2026-03-17 Jerome Weiss

We theoretically investigate the membrane fluctuations of red blood cells with focus laid on the role of the cytoskeleton, viewing the system as a membrane coupled to sparse spring network. This model is exactly solvable and enables us to…

Soft Condensed Matter · Physics 2015-08-31 Wonjune Choi , Juyeon Yi , Yong Woon Kim

Active gels made of cytoskeletal proteins are valuable materials with attractive non-equilibrium properties such as spatial self-organization and self-propulsion. At least four typical routes to spatial patterning have been reported to date…

While actin bundles are used by living cells for structural fortification, the microscopic origin of the elasticity of bundled networks is not understood. Here, we show that above a critical concentration of the actin binding protein…

Soft Condensed Matter · Physics 2007-08-30 O. Lieleg , M. M. A. E. Claessens , C. Heussinger , E. Frey , A. R. Bausch

Motor proteins drive persistent motion and self-organisation of cytoskeletal filaments. However, state-of-the-art microscopy techniques and continuum modelling approaches focus on large length and time scales. Here, we perform…

Soft Condensed Matter · Physics 2024-06-03 Gerrit Vliegenthart , Arvind Ravichandran , Marisol Ripoll , Thorsten Auth , Gerhard Gompper