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The behavior of long DNA molecules in a cell-sized confined space was investigated. We prepared water-in-oil droplets covered by phospholipids, which mimic the inner space of a cell, following the encapsulation of DNA molecules with…

Biological Physics · Physics 2015-04-14 T. Hamada , R. Fujimoto , S. F. Shimobayashi , M. Ichikawa , M. Takagi

The threading of a polymer chain through a small pore is a classic problem in polymer dynamics and underlies nanopore sensing technology. However important experimental aspects of the polymer motion in a solid-state nanopore, such as an…

Biological Physics · Physics 2016-07-18 Nicholas A. W. Bell , Ulrich F. Keyser

An 1d model with time-dependent random hopping is proposed to describe charge transport in DNA. It admits to investigate both diffusion of electrons and their tunneling between different sites in DNA. The tunneling appears to be strongly…

Soft Condensed Matter · Physics 2009-11-07 E. I. Kats , V. V. Lebedev

We confine a dense suspension of motile \textit{Escherichia coli} inside a spherical droplet in a water-in-oil emulsion, creating a "bacterially" propelled droplet. We show that droplets move in a persistent random walk, with a persistence…

Soft Condensed Matter · Physics 2019-12-10 Gabriel Ramos , Maria Luisa Cordero , Rodrigo Soto

Charge migration along DNA molecules has attracted scientific interest for over half a century. Reports on possible high rates of charge transfer between donor and acceptor through the DNA, obtained in the last decade from solution…

Materials Science · Physics 2009-11-10 Danny Porath , Gianaurelio Cuniberti , Rosa Di Felice

Molecular dynamics simulations are used to study the equilibrium distribution of monovalent ions in a nanopore connecting two water reservoirs separated by a membrane, both for the empty pore and that with a single stranded DNA molecule…

Soft Condensed Matter · Physics 2009-11-10 Yitzhak Rabin , Motohiko Tanaka

Cellular responses often require the fast activation or repression of specific genes, which depends on Transcription Factors (TFs) that have to quickly find the promoters of these genes within a large genome. Transcription Factors (TFs)…

Subcellular Processes · Quantitative Biology 2015-09-02 Jérôme Cartailler , Jürgen Reingruber

Analysis of single-molecule micromanipulation experiments of DNA unzipping process shows some features of the force-distance curve, namely two consequent plateaus in the area of ${\sim}12-14pN$ dependent on nucleotide sequence structure, as…

Biological Physics · Physics 2017-02-14 O. O. Zdorevskyi , S. N. Volkov

Structural changes in giant DNA induced by the addition of the flexible polymer PEG were examined by the method of single-DNA observation. In dilute DNA conditions, individual DNA assumes a compact state via a discrete coil-globule…

Soft Condensed Matter · Physics 2016-08-31 M. Kojima , K. Kuobo , K. Yoshikawa

Translation is one of the main steps in the synthesis of proteins. It consists of ribosomes that translate sequences of nucleotides encoded on mRNA into polypeptide sequences of amino acids. Ribosomes bound to mRNA move unidirectionally,…

Subcellular Processes · Quantitative Biology 2022-01-19 Olivier Dauloudet , Izaak Neri , Jean-Charles Walter , Jérôme Dorignac , Frédéric Geniet , Andrea Parmeggiani

A double stranded DNA molecule when pulled with a force acting on one end of the molecule can become either partially or completely unzipped depending on the magnitude of the force F. For a random DNA sequence, the number M of unzipped base…

Soft Condensed Matter · Physics 2007-05-23 Pui-Man Lam , J. C. S. Levy , Hanchen Huang

Organization and maintenance of the chromosomal DNA in living cells strongly depends on the DNA interactions with a plethora of DNA-binding proteins. Single-molecule studies show that formation of nucleoprotein complexes on DNA by such…

Biomolecules · Quantitative Biology 2018-09-05 Artem K. Efremov , Jie Yan

Experiments have revealed correlation-driven behavior of DNA in charged solutions, including charge inversion and condensation. This paper presents calculations of a lattice-gas model of charge inversion for the adsorption of charged dimers…

Soft Condensed Matter · Physics 2019-02-15 Matthew D. Sievert , Marilyn F. Bishop , Tom McMullen

Microscopic mechanisms of positive charge transfer in DNA remain unclear. A quantum state of electron hole in DNA is determined by the competition of a pi-stacking interaction $b$ smearing a charge between different base pairs and…

Soft Condensed Matter · Physics 2008-03-15 D. B. Uskov , A. L. Burin

Recent years have seen a surge of interest in nanopores because such structures show a strong potential for characterizing nanoparticles, proteins, DNA, and even single molecules. These systems have been extensively studied in experiment as…

Soft Condensed Matter · Physics 2016-06-22 Georg Rempfer , Sascha Ehrhardt , Christian Holm , Joost de Graaf

As a key molecule of Life, Deoxyribonucleic acid (DNA) is the focus of numbers of investigations with the help of biological, chemical and physical techniques. From a physical point of view, both experimental and theoretical works have…

Soft Condensed Matter · Physics 2016-05-26 Manoel Manghi , Nicolas Destainville

We study theoretically the feasibility of using transverse electronic transport within a nanopore for rapid DNA sequencing. Specifically, we examine the effects of the environment and detection probes on the distinguishability of the DNA…

Soft Condensed Matter · Physics 2009-11-13 Johan Lagerqvist , Michael Zwolak , Massimiliano Di Ventra

We study theoretically the unzipping of a double stranded DNA from a condensed globule state by an external force. At constant force, we find that the double stranded DNA unzips an at critical force Fc and the number of unzipped monomers M…

Soft Condensed Matter · Physics 2007-05-23 Pui-Man Lam , J. C. Levy

Biomolecular condensates have been shown to play a fundamental role in localizing biochemistry in a cell. RNA is a common constituent of condensates, and can determine their biophysical properties. Functions of biomolecular condensates are…

Subcellular Processes · Quantitative Biology 2023-02-22 Alex Mayer , Grace McLaughlin , Sierra Cole , Amy Gladfelter , Marcus Roper

Electroporation is a physical method to induce the uptake of therapeutic drugs and DNA, by eukaryotic cells and tissues. The phenomena behind electro-mediated membrane permeabilization to plasmid DNA have been shown to be significantly more…

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