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The issue of the nucleation and slow closure mechanisms of non superhelical stress-induced denaturation bubbles in DNA is tackled using coarse-grained MetaDynamics and Brownian simulations. A minimal mesoscopic model is used where the…

Soft Condensed Matter · Physics 2015-04-13 François Sicard , Nicolas Destainville , Manoel Manghi

Structural changes in giant DNA induced by the addition of the flexible polymer PEG were examined by the method of single-DNA observation. In dilute DNA conditions, individual DNA assumes a compact state via a discrete coil-globule…

Soft Condensed Matter · Physics 2016-08-31 M. Kojima , K. Kuobo , K. Yoshikawa

A theory for thermomechanical behavior of homogeneous DNA at thermal equilibrium predicts critical temperatures for denaturation under torque and stretch, phase diagrams for stable B--DNA, supercoiling, optimally stable torque, and the…

Soft Condensed Matter · Physics 2015-05-27 Cristiano Nisoli , A. R. Bishop

Bending of DNA from a straight rod to a circular form in presence of any of the mono-, di-, tri- or tetravalent counterions has been simulated in strong Coulomb coupling environment employing a previously developed energy minimization…

Biological Physics · Physics 2015-05-28 Arup K. Mukherjee

The effective force between two parallel DNA molecules is calculated as a function of their mutual separation for different valencies of counter- and salt ions and different salt concentrations. Computer simulations of the primitive model…

Soft Condensed Matter · Physics 2009-11-10 E. Allahyarov , G. Gompper , H. Löwen

DNA is strong polyelectrolyte macromolecule making metal ions (counterions) condense to a cloud around the double helix. The counterions may be localized outside the macromolecule and inside the minor and major grooves of the double helix.…

Biological Physics · Physics 2022-04-27 Sergiy Perepelytsya , Oleksii Zdorevskyi

When pulled along the axis, double-strand DNA undergoes a large conformational change and elongates roughly twice its initial contour length at a pulling force about 70 pN. The transition to this highly overstretched form of DNA is very…

Soft Condensed Matter · Physics 2008-09-11 Mogurampelly Santosh , Prabal K Maiti

The classical electrostatic interaction between DNA molecules in water in the presence of counterions is reconsidered and we propose it is governed by a modified Poisson-Boltzmann equation. Quantum fluctuations are then studied and shown to…

Quantitative Methods · Quantitative Biology 2008-06-20 Alfredo Iorio , Samik Sen , Siddhartha Sen

The translocation of double-stranded DNA through a solid-state nanopore may either decrease or increase the ionic current depending on the ionic concentration of the surrounding solution. Below a certain crossover ionic concentration, the…

Biological Physics · Physics 2019-09-04 Vivian Wang , Niklas Ermann , Ulrich F. Keyser

We use optical tweezers to perform stretching experiments on DNA molecules when interacting with the drugs daunomycin and ethidium bromide, which intercalate the DNA molecule. These experiments are performed in the low-force regime from…

Biological Physics · Physics 2009-11-13 M. S. Rocha , M. C. Ferreira , O. N. Mesquita

The histone-DNA interaction in the nucleosome is a fundamental mechanism of genomic compaction and regulation, which remains largely unkown despite a growing structural knowledge of the complex. Here, we propose a framework for the…

Biological Physics · Physics 2015-06-23 Sam Meyer , Ralf Everaers

DNA supercoiling is central to many fundamental processes of living organisms. Its average level along the chromosome and over time reflects the dynamic equilibrium of opposite activities of topoisomerases, which are required to relax…

Biomolecules · Quantitative Biology 2023-10-09 Ivan Junier , Elham Ghobadpour , Olivier Espeli , Ralf Everaers

Organization and maintenance of the chromosomal DNA in living cells strongly depends on the DNA interactions with a plethora of DNA-binding proteins. Single-molecule studies show that formation of nucleoprotein complexes on DNA by such…

Biomolecules · Quantitative Biology 2018-09-05 Artem K. Efremov , Jie Yan

The twisting deformation of mechanically stretched DNA molecules is studied by a coarse grained Hamiltonian model incorporating the fundamental interactions that stabilize the double helix and accounting for the radial and angular base pair…

Soft Condensed Matter · Physics 2017-12-15 Marco Zoli

In the demanding biosensing environment, improving selection efficiency strategies has become an issue of great significance. DNA minicircles containing between 200 and 400 base-pairs, also named microDNA, are representative of the…

Biological Physics · Physics 2018-05-14 François Sicard , Nicolas Destainville , Philippe Rousseau , Catherine Tardin , Manoel Manghi

It is a standard exercise in mechanical engineering to infer the external forces and torques on a body from its static shape and known elastic properties. Here we apply this kind of analysis to distorted double-helical DNA in complexes with…

Biomolecules · Quantitative Biology 2009-11-13 Nils B. Becker , Ralf Everaers

We calculate the equation of state of DNA under tension for the case that the DNA features loops. Such loops occur transiently during DNA condensation in the presence of multivalent ions or sliding cationic protein linkers. The…

Biomolecules · Quantitative Biology 2007-05-23 I. M. Kulic , H. Mohrbach , R. Thaokar , H. Schiessel

The closure dynamics of a pre-equilibrated DNA denaturation bubble is studied using both Brownian dynamics simulations and an analytical approach. The numerical model consists of two semi-flexible interacting single strands (ssDNA) and a…

Soft Condensed Matter · Physics 2012-05-31 Anil Kumar Dasanna , Nicolas Destainville , John Palmeri , Manoel Manghi

We study DNA denaturation by integrating elasticity -- as described by the Gaussian network model -- with bond binding energies, distinguishing between different base-pair and stacking energies. We use exact calculation, within the model,…

Soft Condensed Matter · Physics 2016-11-03 Amit Raj Singh , Rony Granek

When multivalent salts are added to dilute DNA solution, the DNA chains condense into bundles of a well-defined size. We explore two physical problems motivated by this phenomenon: the origin of the attractive interactions between the…

Soft Condensed Matter · Physics 2007-05-23 Bae-Yeun Ha , Andrea J. Liu