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We develop a novel peak detection algorithm for the analysis of comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GC$\times$GC-TOF MS) data using normal-exponential-Bernoulli (NEB) and mixture probability…

Applications · Statistics 2014-08-04 Seongho Kim , Ming Ouyang , Jaesik Jeong , Changyu Shen , Xiang Zhang

A double-stranded DNA unravels thermally through intermediate denatured bubble segments. Intrinsically, fluctuations ensue at the bubble boundaries from non-equilibrium (NE) energy exchanges with the environment. However, such details gets…

Soft Condensed Matter · Physics 2018-09-21 Keren Jiang , Faheem Khan , Javix Thomas , Arindam Phani , Thomas Thundat

Sequencing by synthesis is used in many next-generation DNA sequencing technologies. Some of the technologies, especially those exploring the principle of single-molecule sequencing, allow incomplete nucleotide incorporation in each cycle.…

Genomics · Quantitative Biology 2024-05-28 Yong Kong

RNA function is tied to secondary structure, operating through dynamic and heterogeneous structural ensembles. While current analysis tools typically output single static structures or averaged contact maps, chemical probing methods like…

Biomolecules · Quantitative Biology 2026-05-20 Giuseppe Sacco , Jianhui Li , Redmond P. Smyth , Guido Sanguinetti , Giovanni Bussi

The energy landscapes of proteins have evolved to be different from most random heteropolymers. Many studies have concluded that evolutionary selection for rapid and reliable folding to a given structure that is stable at biological…

Disordered Systems and Neural Networks · Physics 2009-11-10 Steven S. Plotkin , Peter G. Wolynes

To characterize the thermodynamical equilibrium of DNA chains interacting with a solution of non-specific binding proteins, a Flory-Huggins free energy model was implemented. We explored the dependence on DNA and protein concentrations of…

Soft Condensed Matter · Physics 2015-11-04 Guillaume Le Treut , François Képès , Henri Orland

We introduce a sequence-dependent parametrization for a coarse-grained DNA model [T. E. Ouldridge, A. A. Louis, and J. P. K. Doye, J. Chem. Phys. 134, 085101 (2011)] originally designed to reproduce the properties of DNA molecules with…

Advances in DNA nanotechnology have stimulated the search for simple motifs that can be used to control the properties of DNA nanostructures. One such motif, which has been used extensively in structures such as polyhedral cages,…

Soft Condensed Matter · Physics 2015-06-10 John S. Schreck , Thomas E. Ouldridge , Flavio Romano , Ard A. Louis , Jonathan P. K. Doye

Many proteins carry out their biological functions by forming the characteristic tertiary structures. Therefore, the search of the stable states of proteins by molecular simulations is important to understand their functions and…

Biomolecules · Quantitative Biology 2015-05-22 Yoshitake Sakae , Tomoyuki Hiroyasu , Mitsunori Miki , Katsuya Ishii , Yuko Okamoto

Cancer development is associated with aberrant DNA methylation, including increased stochastic variability. Statistical tests for discovering cancer methylation biomarkers have focused on changes in mean methylation. To improve the power of…

Methodology · Statistics 2023-06-27 James Y. Dai , Heng Chen , Xiaoyu Wang , Wei Sun , Ying Huang , William M. Grady , Ziding Feng

We introduce a method for predicting RNA folding pathways, with an application to the most important RNA tetraloops. The method is based on the idea that ensembles of three-dimensional fragments extracted from high-resolution crystal…

Biomolecules · Quantitative Biology 2016-11-21 Sandro Bottaro , Alejandro Gil-Ley , Giovanni Bussi

We present a general framework to study the thermodynamic denaturation of double-stranded DNA under superhelical stress. We report calculations of position- and size-dependent opening probabilities for bubbles along the sequence. Our…

Biomolecules · Quantitative Biology 2013-05-29 Daniel Jost , Asif Zubair , Ralf Everaers

DNA samples are often pooled, either by experimental design, or because the sample itself is a mixture. For example, when population allele frequencies are of primary interest, individual samples may be pooled together to lower the cost of…

Quantitative Methods · Quantitative Biology 2013-02-07 Darren Kessner , Tom Turner , John Novembre

The melting curves of short heterogeneous DNA chains in solution are calculated on the basis of statistical thermodynamics and compared to experiments. The computation of the partition function is based on the Peyrard-Bishop hamiltonian,…

Biological Physics · Physics 2007-05-23 Alessandro Campa , Andrea Giansanti

This contribution presents a review of our present theoretical as well as experimental knowledge of different fluctuation observables relevant to nuclear multifragmentation. The possible connection between the presence of a fluctuation peak…

Nuclear Experiment · Physics 2009-11-11 F. Gulminelli , M. D'Agostino

We report studies of the equilibrium and the dynamics of a general set of lattice models which capture the essence of the force-induced or mechanical DNA unzipping transition. Besides yielding the whole equilibrium phase diagram in the…

Soft Condensed Matter · Physics 2009-11-07 D. Marenduzzo , S. M. Bhattacharjee , A. Maritan , E. Orlandini , F. Seno

We develop topological methods for analyzing difference topology experiments involving 3-string tangles. Difference topology is a novel technique used to unveil the structure of stable protein-DNA complexes involving two or more DNA…

Geometric Topology · Mathematics 2016-01-20 Isabel K. Darcy , John Luecke , Mariel Vazquez

Genomic phenotypes, such as DNA methylation and chromatin accessibility, can be used to characterize the transcriptional and regulatory activity of DNA within a cell. Recent technological advances have made it possible to measure such…

Methodology · Statistics 2016-11-15 Jean Morrison , Noah Simon , Daniela Witten

High-density DNA arrays, used to monitor gene expression at a genomic scale, have produced vast amounts of information which require the development of efficient computational methods to analyze them. The important first step is to extract…

Biological Physics · Physics 2009-10-31 G. Getz , E. Levine , E. Domany , M. Q. Zhang

Mass spectrometry provides a high-throughput way to identify proteins in biological samples. In a typical experiment, proteins in a sample are first broken into their constituent peptides. The resulting mixture of peptides is then subjected…

Applications · Statistics 2010-11-10 Qunhua Li , Michael J. MacCoss , Matthew Stephens
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