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Circadian clocks are the central timekeepers of life, allowing cells to anticipate changes between day and night. Experiments in recent years have revealed that circadian clocks can be highly stable, raising the question how reliably they…

Molecular Networks · Quantitative Biology 2016-06-22 Michele Monti , Pieter Rein ten Wolde

Circadian clocks are biochemical oscillators that allow organisms to estimate the time of the day. These oscillators are inherently noisy due to the discrete nature of the reactants and the stochastic character of their interactions. To…

Biological Physics · Physics 2018-03-21 Michele Monti , David K. Lubensky , Pieter Rein ten Wolde

The circadian clocks keeping time of day in many living organisms rely on self-sustained biochemical oscillations which can be entrained by external cues, such as light, to the 24-hour cycle induced by Earth rotation. However, environmental…

Quantitative Methods · Quantitative Biology 2015-05-20 Benjamin Pfeuty , Quentin Thommen , Marc Lefranc

Synthetic biology sets out to implement new functions in cells, and to develop a deeper understanding of biological design principles. In 2000, Elowitz and Leibler showed that by rational design of the reaction network, and using existing…

Biological Physics · Physics 2017-05-17 Joris Paijmans , David K Lubensky , Pieter Rein ten Wolde

Genetic oscillators, such as circadian clocks, are constantly perturbed by molecular noise arising from the small number of molecules involved in gene regulation. One of the strongest sources of stochasticity is the binary noise that arises…

Biological Physics · Physics 2016-01-06 Sargis Karapetyan , Nicolas E. Buchler

Circadian clocks play a pivotal role in orchestrating numerous physiological and developmental events. Waveform shapes of the oscillations of protein abundances can be informative about the underlying biochemical processes of circadian…

Subcellular Processes · Quantitative Biology 2018-11-27 Hang-Hyun Jo , Yeon Jeong Kim , Jae Kyoung Kim , Mathias Foo , David E. Somers , Pan-Jun Kim

The development of new techniques to quantitatively measure gene expression in cells has shed light on a number of systems that display oscillations in protein concentration. Here we review the different mechanisms which can produce…

Molecular Networks · Quantitative Biology 2009-11-13 G. Tiana , S. Krishna , S. Pigolotti , M. H. Jensen , K. Sneppen

Negative and positive transcriptional feedback loops are present in natural and synthetic genetic oscillators. A single gene with negative transcriptional feedback needs a time delay and sufficiently strong nonlinearity in the transmission…

Molecular Networks · Quantitative Biology 2011-12-23 Jesús M. Miró-Bueno , Alfonso Rodríguez-Patón

We study the noise characteristics of stochastic oscillations in protein number dynamics of simple genetic oscillatory systems. Using the three-component negative feedback transcription regulatory system called the repressilator as a…

Molecular Networks · Quantitative Biology 2015-03-13 Byungjoon Min , K. -I. Goh , I. -M. Kim

To estimate the time, many organisms, ranging from cyanobacteria to animals, employ a circadian clock which is based on a limit-cycle oscillator that can tick autonomously with a nearly 24h period. Yet, a limit-cycle oscillator is not…

Molecular Networks · Quantitative Biology 2018-08-22 Michele Monti , David K Lubensky , Pieter Rein ten Wolde

Ring oscillators are biochemical circuits consisting of a ring of interactions capable of sustained oscillations. The non-linear interactions between genes hinder the analytical insight into their function, usually requiring computational…

Molecular Networks · Quantitative Biology 2018-02-19 Karen M. Page , Ruben Perez-Carrasco

Circadian (~24hr) clocks are self-sustained endogenous oscillators with which organisms keep track of daily and seasonal time. Circadian clocks frequently rely on interlocked transcriptional- translational feedback loops to generate rhythms…

Molecular Networks · Quantitative Biology 2016-04-14 Jae Kyoung Kim

The development of systemic approaches in biology has put emphasis on identifying genetic modules whose behavior can be modeled accurately so as to gain insight into their structure and function. However most gene circuits in a cell are…

Molecular Networks · Quantitative Biology 2010-12-10 Quentin Thommen , Benjamin Pfeuty , Pierre-Emmanuel Morant , Florence Corellou , François-Yves Bouget , Marc Lefranc

Gene Regulatory Networks(GRNs) with feedback are essential components of many cellular processes and may exhibit oscillatory behavior. Analyzing such systems becomes increasingly complex as the number of components increases. Since gene…

Molecular Networks · Quantitative Biology 2026-04-22 Manuel Eduardo Hernández-García , Jorge Velázquez-Castro

Intracellular transmission of information via chemical and transcriptional networks is thwarted by a physical limitation: the finite copy number of the constituent chemical species introduces unavoidable intrinsic noise. Here we provide a…

Molecular Networks · Quantitative Biology 2010-08-27 Andrew Mugler , Aleksandra M. Walczak , Chris H. Wiggins

The suprachiasmatic nucleus (SCN), also known as the circadian master clock, consists of a large population of oscillator neurons. Together, these neurons produce a coherent signal that drives the body's circadian rhythms. What properties…

Neurons and Cognition · Quantitative Biology 2023-01-04 Stéphanie M. C. Abo , José A. Carrillo , Anita T. Layton

The genetic repressilator circuit consists of three transcription factors, or repressors, which negatively regulate each other in a cyclic manner. This circuit was synthetically constructed on plasmids in {\it Escherichia coli} and was…

Molecular Networks · Quantitative Biology 2009-11-13 Adiel Loinger , Ofer Biham

In biochemical signaling, information is often encoded in oscillatory signals. However, the advantages of such a coding strategy over an amplitude encoding scheme of constant signals remain unclear. Here we study the dynamics of a simple…

Subcellular Processes · Quantitative Biology 2012-03-09 Filipe Tostevin , Wiet de Ronde , Pieter Rein ten Wolde

Genetic oscillators are present in the cells of many organisms and control several biological processes. The common feature of such oscillators is the presence of a protein which represses the transcription of its own gene. Recently, it has…

Molecular Networks · Quantitative Biology 2017-06-07 Nahuel Almeira , Sebastian Risau Gusman

Protein sequestration motifs appear in many biological regulatory networks and introduce special properties into the network dynamics. Sequestration can be described as a mode of inactivation of a given protein by its binding to a second…

Dynamical Systems · Mathematics 2025-06-05 Benjamin Böbel , Madalena Chaves , Jean-Luc Gouzé
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