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Optogenetic modulation of adenosine triphosphatase (ATPase) expression represents a novel approach to maximize bioprocess efficiency by leveraging enforced adenosine triphosphate (ATP) turnover. In this study, we experimentally implement a…

Multisite protein phosphorylation plays a pivotal role in regulating cellular signaling and decision-making processes. In this study, we focus on the mathematical underpinnings and informational aspects of sequential, distributive…

Molecular Networks · Quantitative Biology 2025-07-22 Iman Tavassoly , Adel Mehrpooya , Parsa Mirlohi , Zahra Abbaspourasadollah

Unstructured proteins can modulate cellular responses to environmental conditions by undergoing coil-globule transitions and phase separation. However, the molecular mechanisms of these phenomena still need to be fully understood. Here, we…

Soft Condensed Matter · Physics 2023-06-28 Bernat Durà Faulí , Valentino Bianco , Giancarlo Franzese

Kinesin and related motor proteins utilize ATP fuel to propel themselves along the external surface of microtubules in a processive and directional fashion. We show that the observed step-like motion is possible through time varying charge…

Biomolecules · Quantitative Biology 2007-05-23 A. Ciudad , J. M. Sancho , G. P. Tsironis

Living organisms continuously harness energy to perform complex functions for their adaptation and survival while part of that energy is dissipated in the form of heat or chemical waste. Determining the energetic cost and the efficiency of…

Molecular Networks · Quantitative Biology 2023-08-07 Valérie Voorsluijs , Francesco Avanzini , Gianmaria Falasco , Massimiliano Esposito , Alexander Skupin

Radiotherapy can effectively kill malignant cells, but the doses required to cure cancer patients may inflict severe collateral damage to adjacent healthy tissues. Hyperthermia (HT) is a promising option to improve the outcome of radiation…

A ubiquitous building block of signaling pathways is a cycle of covalent modification (e.g., phosphorylation and dephosphorylation in MAPK cascades). Our paper explores the kind of information processing and filtering that can be…

Molecular Networks · Quantitative Biology 2015-05-13 Carlos Gomez-Uribe , George C. Verghese , Leonid A. Mirny

Allostery plays a fundament role in most biological processes. However, little theory is available to describe it outside of two-state models. Here we use a statistical mechanical approach to show that the allosteric coupling between two…

Biological Physics · Physics 2017-04-05 Michel A. Cuendet , Harel Weinstein , Michael V. LeVine

Optimizing information transmission across a network is an essential task for controlling and manipulating generic information-processing systems. Here, we show how topological amplification effects in scale-free networks of signaling…

Physics and Society · Physics 2016-05-04 Pedro J. Martínez , Ricardo Chacón

Most sensory cells use surface receptors to detect environmental stimuli and initiate downstream signaling. Cooperative interactions among sensory receptors is known to play a crucial role in enhancing the sensitivity of biochemical…

Molecular Networks · Quantitative Biology 2014-01-15 Jianmin Sun , Michael Grabe

The motility of cilia and flagella is driven by thousands of dynein motors that hydrolyze adenosine triphosphate (ATP). Despite decades of genetic, biochemical, structural and biophysical studies, some aspects of ciliary motility remain…

Biological Physics · Physics 2015-12-22 Daniel T. N. Chen , Michael Heymann , Seth Fraden , Daniela Nicastro , Zvonimir Dogic

Many signalling functions in molecular biology require proteins bind to substrates such as DNA in response to environmental signals such as the simultaneous binding to a small molecule. Examples are repressor proteins which may transmit…

Biomolecules · Quantitative Biology 2009-11-10 Rhoda J. Hawkins , Thomas C. B. McLeish

We study the thermodynamic properties of a system of two-level dipoles that are coupled ultrastrongly to a single cavity mode. By using exact numerical and approximate analytical methods, we evaluate the free energy of this system at…

Quantum Physics · Physics 2020-09-30 Philipp Pilar , Daniele De Bernardis , Peter Rabl

Multisite covalent modification of proteins is omnipresent in eukaryotic cells. A well-known example is the mitogen-activated protein kinase (MAPK) cascade, where in each layer of the cascade a protein is phosphorylated at two sites. It has…

Molecular Networks · Quantitative Biology 2015-05-13 Koichi Takahashi , Sorin Tanase-Nicola , Pieter Rein ten Wolde

The respective roles of local and nonlocal interactions in the thermodynamic cooperativity of proteins are investigated using continuum (off-lattice) native-centric G\=o-like models with a coarse-grained C$_\alpha$ chain representation. We…

Statistical Mechanics · Physics 2007-05-23 Michael Knott , Huseyin Kaya , Hue Sun Chan

We show that active transport processes in biological systems can be understood through a local equilibrium description formulated at the mesoscale, the scale to describe stochastic processes. This new approach uses the method established…

Soft Condensed Matter · Physics 2007-05-23 S. Kjelstrup , J. M. Rubi , D. Bedeaux

Under many conditions, biomolecules and nanoparticles associate by means of attractive bonds, due to hydrophobic attraction. Extracting the microscopic association or dissociation rates from experimental data is complicated by the…

Soft Condensed Matter · Physics 2011-10-13 Alessio Zaccone , Jerome J. Crassous , Benjamin Béri , Matthias Ballauff

Active ion transport is very critical for living cells to maintain and regular internal and external environment. It is known that adenosine 5'-triphosphate (ATP) is a general energy source that applies bond energy for pumps to overcome ion…

Subcellular Processes · Quantitative Biology 2023-02-01 Xiang Zou

The process of protein folding from an unfolded state to a biologically active, folded conformation is governed by many parameters e.g the sequence of amino acids, intermolecular interactions, the solvent, temperature and chaperon…

Soft Condensed Matter · Physics 2010-10-19 Pragya Shukla

We examine changes in the picosecond structural dynamics with irreversible photobleaching of red fluorescent proteins mCherry, mOrange2 and TagRFP-T. Measurements of the protein dynamical transition using terahertz time-domain spectroscopy…

Biological Physics · Physics 2019-06-04 M. Xu , D. K. George , R. Jimenez , A. G. Markelz