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When pulled along its axis, double-stranded DNA elongates abruptly at a force of about 65 pN. Two physical pictures have been developed to describe this overstretched state. The first proposes that strong forces induce a phase transition to…

Statistical Mechanics · Physics 2015-06-25 Stephen Whitelam , Sander Pronk , Phillip L. Geissler

We present an elastic model of B-form DNA as a stack of thin, rigid plates or base pairs that are not permitted to deform. The symmetry of DNA and the constraint of plate rigidity limit the number of bulk elastic constants contributing to a…

Soft Condensed Matter · Physics 2009-10-30 C. S. O'Hern , Randall D. Kamien , T. C. Lubensky , Philip Nelson

From the per unit length free energy for DNA under tension, we have calculated an effective contour length dependent persistence length for short DNA. This effective persistence length results from the enhanced fluctuations in short DNA. It…

Soft Condensed Matter · Physics 2017-06-07 Pui-Man Lam , Yi Zhen

We use optical tweezers to perform stretching experiments on DNA molecules when interacting with the drugs daunomycin and ethidium bromide, which intercalate the DNA molecule. These experiments are performed in the low-force regime from…

Biological Physics · Physics 2009-11-13 M. S. Rocha , M. C. Ferreira , O. N. Mesquita

We propose a formalism for deriving force-elongation and elongation-force relations for flexible chain molecules from analytical expressions for their radial distribution function, which provides insight into the factors controlling the…

Soft Condensed Matter · Physics 2021-02-03 Ralf Everaers , Nils B. Becker , Angelo Rosa

We use an elastic rod model with contact to study the extension versus rotation diagrams of single supercoiled DNA molecules. We reproduce quantitatively the supercoiling response of overtwisted DNA and, using experimental data, we get an…

Biomolecules · Quantitative Biology 2013-05-29 Sebastien Neukirch

Helical molecules change their twist number under the effect of a mechanical load. We study the twist-stretch relation for a set of short DNA molecules modeled by a mesoscopic Hamiltonian. Finite temperature path integral techniques are…

Soft Condensed Matter · Physics 2017-09-19 Marco Zoli

We present a perturbation theory to find the response of an anisotropic DNA to the external tension. It is shown that the anisotropy has a nonzero but small contribution to the force-extension curve of the DNA. Thus an anisotropic DNA…

Soft Condensed Matter · Physics 2016-09-08 B. Eslami-Mossallam , M. R. Ejtehadi

Recent experimental data indicate that the elastic wormlike rod model of DNA that works well on long length scales may break down on shorter scales relevant to biology. According to Noy and Golestanian (Phys. Rev. Lett. 109, 228101, 2012)…

Biomolecules · Quantitative Biology 2013-11-12 Alexey K. Mazur

Three coarse-grained models of the double-stranded DNA are proposed and compared in the context of mechanical manipulation such as twisting and various schemes of stretching. The models differ in the number of effective beads (between two…

Biomolecules · Quantitative Biology 2015-05-13 Szymon Niewieczerzał , Marek Cieplak

The probability distributions for bending angles in double helical DNA obtained in all-atom molecular dynamics simulations are compared with theoretical predictions. The computed distributions remarkably agree with the worm-like chain…

Biomolecules · Quantitative Biology 2009-11-13 Alexey K. Mazur

A revised Marko-Siggia elastic model for DNA double helix [Macromolecules 27, 981 (1994)] is proposed, which includes the WLC bending energy and a new chiral twisting energy term. It is predicted that the mean helical repeat length (HRL)…

Soft Condensed Matter · Physics 2009-10-31 Zhou Haijun , Ou-Yang Zhong-can

The influence of tension on DNA looping has been studied both experimentally and theoretically in the past. However, different theoretical models have yielded different predictions, leaving uncertainty about their validity. We briefly…

Statistical Mechanics · Physics 2025-01-17 Wout Laeremans , Wouter G Ellenbroek

In order to interpret recent experimental studies of the dependence of conductance of ds-DNA as the DNA is pulled from the 3'end1-3'end2 ends, which find a sharp conductance jump for a very short (4.5 %) stretching length, we carried out…

Soft Condensed Matter · Physics 2016-08-26 Saientan Bag , Santosh Mogurampelly , William A Goddard , Prabal K Maiti

The mechanism of threshold elongation (overstretching) of DNA macromolecules under the action of external force is studied within the framework of phenomenological approach. When considering the task it is taken into account that…

Biomolecules · Quantitative Biology 2016-02-22 Sergey N. Volkov

In this work we propose a model for DNA double helix within the tight-binding framework that incorporates the helicity of the molecules. We have studied localization properties of three DNAsequences,the periodic poly(dG)-poly(dC) and…

Mesoscale and Nanoscale Physics · Physics 2015-07-27 Sourav Kundu , S. N. Karmakar

We review statistical-mechanical theories of single-molecule micromanipulation experiments on nucleic acids. First, models for describing polymer elasticity are introduced. We then review how these models are used to interpret…

Soft Condensed Matter · Physics 2007-05-23 S. Cocco , J. F. Marko , R. Monasson

The double-helical structure of DNA results from canonical base pairing and stacking interactions. However, variations from steady-state conformations result from mechanical perturbations in cells. These different topologies have…

Biomolecules · Quantitative Biology 2019-04-05 Jack W Shepherd , Robert J Greenall , Matt I J Probert , Agnes Noy , Mark C Leake

The recently proposed compressed backbone theory suggested that the intrinsic curvature in DNA can result from a geometric mismatch between the specific backbone length and optimal base stacking orientations. It predicted that the curvature…

Biomolecules · Quantitative Biology 2007-05-23 Dimitri E. Kamashev , Alexey K. Mazur

Many structures inside the cell such as nucleosomes and protein-mediated DNA loops contain sharply bent double-stranded (ds) DNA. Therefore, the energetics of strong dsDNA bending constitutes an essential part of cellular thermodynamics.…

Biological Physics · Physics 2014-11-05 Tung T. Le , Harold D. Kim