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We develop a continuum elastic approach to examining the bending mechanics of semiflexible filaments with a local internal degree of freedom that couples to the bending modulus. We apply this model to study the nonlinear mechanics of a…

Soft Condensed Matter · Physics 2013-02-12 Arthur A. Evans , Alex J. Levine

We study the force-induced unfolding of random disordered RNA or single-stranded DNA polymers. The system undergoes a second order phase transition from a collapsed globular phase at low forces to an extensive necklace phase with a…

Soft Condensed Matter · Physics 2009-11-07 M. Mueller , F. Krzakala , M. Mezard

Recent experiments have shown that the force-extension (F-x) curve for single-stranded DNA (ssDNA) consisting only of adenine [poly(dA)] is significantly different from thymine [poly(dT)]. Here, we show that the base stacking interaction is…

Soft Condensed Matter · Physics 2015-05-13 Garima Mishra , Debaprasad Giri , Sanjay Kumar

We considered a dsDNA polymer in which distribution of bases are random at the base pair level but ordered at a length of 18 base pairs and calculated its force elongation behaviour in the constant extension ensemble. The unzipping force…

Soft Condensed Matter · Physics 2007-05-23 Navin Singh , Yashwant Singh

Single molecule experiments on B-DNA stretching have revealed one or two structural transitions, when increasing the external force. They are characterized by a sudden increase of DNA contour length and a decrease of the bending rigidity.…

Soft Condensed Matter · Physics 2012-10-25 Manoel Manghi , Nicolas Destainville , John Palmeri

Tethered particle experiments use light microscopy to measure the position of a micrometer-sized bead tethered to a microscope slide via a ~micrometer length polymer, in order to infer the behavior of the invisible polymer. Currently, this…

Biological Physics · Physics 2009-11-13 John F. Beausang , Chiara Zurla , Carlo Manzo , David Dunlap , Laura Finzi , Philip C. Nelson

A double stranded DNA molecule under the stress of a pulling force acting on the strand terminals exhibits a partially denatured structure or can be completely unzipped depending the magnitude of the pulling force. A scaling argument for…

Statistical Mechanics · Physics 2009-09-25 Jeff Z. Y. Chen

The mechanical properties of DNA play a critical role in many biological functions. For example, DNA packing in viruses involves confining the viral genome in a volume (the viral capsid) with dimensions that are comparable to the DNA…

Overstretching of B-DNA is currently understood as force-induced melting. Depending on the geometry of the stretching experiment, the force threshold for the overstretching transition is around 65 or 110 pN. Although the mechanisms behind…

Biological Physics · Physics 2019-03-06 Nikos Theodorakopoulos

Negatively charged DNA can be compacted by positively charged dendrimers and the degree of compaction is a delicate balance between the strength of the electrostatic interaction and the elasticity of DNA. We report various elastic…

Biological Physics · Physics 2013-07-25 Santosh Mogurampelly , Bidisha Nandy , Roland R. Netz , Prabal K. Maiti

A versatile approach to modeling the conformations and energetics of DNA loops is presented. The model is based on the classical theory of elasticity, modified to describe the intrinsic twist and curvature of DNA, the DNA bending…

Biological Physics · Physics 2007-05-23 Alexander Balaeff , L. Mahadevan , Klaus Schulten

A theory is presented of the longitudinal dispersion of DNA under equilibrium confined in a nanochannel. Orientational fluctuations of the DNA chain build up to give rise to substantial fluctuations of the coil in the longitudinal direction…

Soft Condensed Matter · Physics 2009-11-18 Theo Odijk

We have performed single molecule stretching experiments and dynamic light scattering (DLS) in order to characterize the interaction between the DNA molecule and the fluorescent stain GelRed. The results from single molecule stretching show…

Biological Physics · Physics 2014-08-14 F. A. P. Crisafuli , E. B. Ramos , M. S. Rocha

While analyzing the persistence length of DNA atomistic simulations Wu et al. [J. Chem. Phys. 142, 125103 (2015)] introduced an empirical formula to account for the observed length-dependence. In particular they found that the persistence…

Soft Condensed Matter · Physics 2021-10-26 Midas Segers , Enrico Skoruppa , Jan A. Stevens , Merijn Vangilbergen , Aderik Voorspoels , Enrico Carlon

Theoretical results for the extension of a polymer confined to a channel are usually derived in the limit of infinite contour length. But experimental studies and simulations of DNA molecules confined to nanochannels are not necessarily in…

Soft Condensed Matter · Physics 2015-12-23 T. St Clere Smithe , V. Iarko , A. Muralidhar , E. Werner , K. D. Dorfman , B. Mehlig

Genomic DNA is constantly subjected to various mechanical stresses arising from its biological functions and cell packaging. If the local mechanical properties of DNA change under torsional and tensional stress, the activity of…

Biomolecules · Quantitative Biology 2015-05-28 Alexey K. Mazur

Minuscule molecular forces can transform DNA into a structure that is elongated by more than half its original length. We demonstrate that this pronounced conformational transition is of relevance to ongoing experimental and theoretical…

Materials Science · Physics 2010-07-06 Paul Maragakis , Ryan Lee Barnett , Efthimios Kaxiras , Marcus Elstner , Thomas Frauenheim

We present a self-contained theory for the mechanical response of DNA in single molecule experiments. Our model is based on a 1D continuum description of the DNA molecule and accounts both for its elasticity and for DNA-DNA electrostatic…

Biomolecules · Quantitative Biology 2015-05-13 N. Clauvelin , B. Audoly , S. Neukirch

Extreme deformations of the DNA double helix attracted a lot of attention during the past decades. Particularly, the determination of the persistence length of DNA with extreme local disruptions, or kinks, has become a crucial problem in…

Biological Physics · Physics 2018-10-30 Kyryło A. Simonov

The mechanism of threshold elongation (overstretching) of DNA macromolecules under the action of external force is studied within the framework of phenomenological approach. When considering the task it is taken into account that…

Biomolecules · Quantitative Biology 2016-02-22 Sergey N. Volkov