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The bacterium Escherichia coli initiates replication once per cell cycle at a precise volume per origin and adds an on average constant volume between successive initiation events, independent of the initiation size. Yet, a molecular model…

Biological Physics · Physics 2022-11-23 Mareike Berger , Pieter Rein ten Wolde

Being a dual purpose enzyme, the DNA polymerase is responsible for elongation of the newly formed DNA strand as well as cleaving the erroneous growth in case of a misincorporation. The efficiency of replication depends on the coordination…

Soft Condensed Matter · Physics 2021-10-13 M Sahoo , Arsha N , P R Baral , S Klumpp

Many biological molecular motors and machines are driven by chemical reactions that occur in specific catalytic sites. We study whether the arrival of molecules to such an active site can be accelerated by the presence of a nearby inactive…

Statistical Mechanics · Physics 2025-01-06 Hila Katznelson , Saar Rahav

Oxidative DNA single strand breaks arise continuously in cells and defects in their repair have been implicated in neurological disease. While much progress has been made in understanding how chromosomal single strand breaks are repaired…

Subcellular Processes · Quantitative Biology 2012-02-03 Arman Nabatiyan , Zhihong Zeng , Keith W. Caldecott

We present a procedure for enhanced sampling of molecular dynamics simulations through informed stochastic resetting. Many phenomena, such as protein folding and crystal nucleation, occur over time scales that are inaccessible in standard…

DNA-coated colloids can crystallize into a multitude of lattices, ranging from face-centered cubic to diamond and thereby contribute to our understanding of crystallization and open avenues to producing structures with useful photonic…

Soft Condensed Matter · Physics 2026-04-17 Pepijn G. Moerman , Huang Fang , Thomas E. Videbæk , W. Benjamin Rogers , Rebecca Schulman

The ability to manipulate individual atoms and molecules using a scanning tunnelling microscope (STM) has been crucial for the development of a vast array of atomic scale devices and structures ranging from nanoscale motors and switches to…

Strongly Correlated Electrons · Physics 2021-02-03 Léonard Desvignes , Vasily S. Stolyarov , Marco Aprili , Freek Massee

We develop revised theoretical ideas on the mechanism by which the transcription factor proteins locate their specific binding sites on DNA faster than the three-dimensional (3D) diffusion controlled rate limit. We demonstrate that the…

Biological Physics · Physics 2014-09-22 Rajamanickam Murugan

DNA strand displacement (DSD) reactions have been used to construct chemical reaction networks in which species act catalytically at the level of the overall stoichiometry of reactions. These effective catalytic reactions are typically…

Molecular Networks · Quantitative Biology 2020-05-26 Antti Lankinen , Ismael Mullor Ruiz , Thomas E. Ouldridge

Background: In Escherichia coli, overlapping rounds of DNA replication allow the bacteria to double in faster times than the time required to copy the genome. The precise timing of initiation of DNA replication is determined by a regulatory…

Polymerization of RNA from a template DNA is carried out by a molecular machine called RNA polymerase (RNAP). It also uses the template as a track on which it moves as a motor utilizing chemical energy input. The time it spends at each…

Statistical Mechanics · Physics 2015-05-13 Tripti Tripathi , Gunter M. Schütz , Debashish Chowdhury

Simulations can help unravel the complicated ways in which molecular structure determines function. Here, we use molecular simulations to show how slight alterations of a molecular motor's structure can cause the motor's typical dynamical…

Statistical Mechanics · Physics 2023-08-21 Alex Albaugh , Geyao Gu , Todd R. Gingrich

We review statistical-mechanical theories of single-molecule micromanipulation experiments on nucleic acids. First, models for describing polymer elasticity are introduced. We then review how these models are used to interpret…

Soft Condensed Matter · Physics 2007-05-23 S. Cocco , J. F. Marko , R. Monasson

A new dual plasma coating process to produce platinum-free catalysts for the oxygen reduction reaction in a fuel cell is introduced. The catalysts thus produced were analysed with various methods. Electrochemical characterisation was…

A DNA polymerase (DNAP) replicates a template DNA strand. It also exploits the template as the track for its own motor-like mechanical movement. In the polymerase mode it elongates the nascent DNA by one nucleotide in each step. But,…

Subcellular Processes · Quantitative Biology 2013-10-16 Ajeet K. Sharma , Debashish Chowdhury

Thermalcycler were extensively used machine for amplify DNA sample. One of the major problems in the working time was that it spent most of time for cooling and heating. In order to improve the efficient, this study presented a novel method…

Other Computer Science · Computer Science 2007-11-29 Ch. -Heng Chien , Hui-Min Yu

Experimental evidences show that in gene transcription, RNA polymerase has the possibility to be stalled at certain position of the transcription template. This may be due to the template damage, or protein barriers. Once stalled,…

Biological Physics · Physics 2015-02-10 Jingwei Li , Yunxin Zhang

Solid state nanopores have emerged as powerful tools for single-molecule sensing, yet the rapid uncontrolled translocation of the molecule through the pore remains a key limitation. We have previously demonstrated that an active…

This computational study introduces a theoretical framework for practical, electrochemical fuel generation displaying exponential product yields as functions of time. Exponential reaction scaling is simulated through an autocatalytic cycle…

Chemical Physics · Physics 2026-02-03 Peter Agbo

The quite recent technological rise in molecular biology allowed single molecule manipulation experiments, where molecule stretching plays a primary role. In order to understand the experimental data, it is felt the urge of some physical…

Biological Physics · Physics 2011-01-28 Francesco A. Massucci , Isaac Pérez Castillo , Conrad Pérez Vicente