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The two strands of the DNA double helix can be `unzipped' by application of 15 pN force. We analyze the dynamics of unzipping and rezipping, for the case where the molecule ends are separated and re-approached at constant velocity. For…

Soft Condensed Matter · Physics 2009-11-07 S. Cocco , R. Monasson , J. F. Marko

The possibility of detecting mutations in a DNA from force measurements (as a first step towards sequence analysis) is discussed theoretically based on exact calculations. The force signal is associated with the domain wall separating the…

Statistical Mechanics · Physics 2009-11-07 Somendra M. Bhattacharjee , D. Marenduzzo

We study a class of micromanipulation experiments, exemplified by the pulling apart of the two strands of double-stranded DNA (dsDNA). When the pulling force is increased to a critical value, an ``unzipping'' transition occurs. For random…

Soft Condensed Matter · Physics 2009-10-31 David K. Lubensky , David R. Nelson

We study the melting of a double stranded DNA in the presence of stretching forces, via 3D Monte-Carlo simulations, exactly solvable models and heuristic arguments. The resulting force-temperature phase diagram is dramatically different for…

Soft Condensed Matter · Physics 2008-07-22 D. Marenduzzo , A. Maritan , E. Orlandini , F. Seno , A. Trovato

We separate double stranded lambda phage DNA by applying a fixed force at a constant temperature ranging from 15C to 50C, and measure the minimum force required to separate the two strands, providing the first experimental determination of…

Soft Condensed Matter · Physics 2009-11-10 C. Danilowicz , Y. Kafri , R. S. Conroy , V. W. Coljee , J. Weeks , M. Prentiss

We show how single-molecule unzipping experiments can provide strong evidence that the zero-force melting transition of long molecules of natural dsDNA should be classified as a phase transition of the higher-order type (continuous). We…

Soft Condensed Matter · Physics 2007-05-23 C. Brian Roland , Kristi Adamson Hatch , Mara Prentiss , Eugene I. Shakhnovich

We have studied the separation of a double stranded DNA (dsDNA), which is driven either by the temperature or force. By monitoring the probability of opening of entire base pairs along the chain, we show that the opening of a dsDNA depends…

Soft Condensed Matter · Physics 2011-05-03 Shikha Srivastava , Navin Singh

We study theoretically the mechanical failure of a simple model of double stranded DNA under an applied shear. Starting from a more microscopic Hamiltonian that describes a sheared DNA, we arrive at a nonlinear generalization of a ladder…

Soft Condensed Matter · Physics 2009-04-13 Buddhapriya Chakrabarti , David R. Nelson

A model of self avoiding walks with suitable constraint has been developed to study the effect of temperature on a single stranded DNA (ssDNA) in the constant force ensemble. Our exact calculations for small chains show that the extension…

Statistical Mechanics · Physics 2009-11-13 Sanjay Kumar , Garima Mishra

The flexibility and the extension along the direction of the force are shown to be related to the bubble number fluctuation and the average number of bubbles respectively, when the strands of the DNA are subjected to a force along the same…

Soft Condensed Matter · Physics 2021-05-21 Debjyoti Majumdar

The force induced unzipping transition of a double stranded DNA is considered from a purely thermodynamic point of view. This analysis provides us with a set of relations that can be used to test microscopic theories and experiments. The…

Soft Condensed Matter · Physics 2015-06-18 Poulomi Sadhukhan , Somendra M. Bhattacharjee

We report studies of the equilibrium and the dynamics of a general set of lattice models which capture the essence of the force-induced or mechanical DNA unzipping transition. Besides yielding the whole equilibrium phase diagram in the…

Soft Condensed Matter · Physics 2009-11-07 D. Marenduzzo , S. M. Bhattacharjee , A. Maritan , E. Orlandini , F. Seno

The unzipping of a double stranded DNA whose ends are subjected to a time dependent periodic force with frequency $\omega$ and amplitude $G$ is studied using Monte Carlo simulations. We obtain the dynamical order parameter, $Q$, defined as…

Soft Condensed Matter · Physics 2019-06-17 M. Suman Kalyan , Rajeev Kapri

Double-stranded DNA `overstretches' at a pulling force of about 65 pN, increasing in length by a factor of 1.7. The nature of the overstretched state is unknown, despite its considerable importance for DNA's biological function and…

Statistical Mechanics · Physics 2009-11-13 Stephen Whitelam , Sander Pronk , Phillip L. Geissler

We discuss how a balance can be established between the force acting to eject DNA from viral capsids and the force resisting its entry into a colloidal suspension which mimics the host cell cytoplasm. The ejection force arises from the…

Biological Physics · Physics 2007-05-23 A. Evilevitch , M. Castelnovo , C. M. Knobler , W. M. Gelbart

A simple model of DNA based on two interacting polymers has been used to study the unzipping of a double stranded DNA subjected to a periodic force. We propose a dynamical transition, where without changing the physiological condition, it…

Biological Physics · Physics 2015-06-11 Sanjay Kumar , Garima Mishra

The effect of electrostatic interactions on the stretching of DNA is investigated using a simple worm like chain model. In the limit of small force there are large conformational fluctuations which are treated using a self-consistent…

Soft Condensed Matter · Physics 2009-10-31 Namkyung Lee , D. Thirumalai

We investigate irreversibility and dissipation in single molecules that cooperatively fold/unfold in a two state manner under the action of mechanical force. We apply path thermodynamics to derive analytical expressions for the average…

Statistical Mechanics · Physics 2009-02-25 Maria Manosas , Alessandro Mossa , Nuria Forns , Josep Maria Huguet , Felix Ritort

We study the unzipping of double stranded DNA (dsDNA) by applying a pulling force at a fraction $s$ $(0 \le s \le 1)$ from the anchored end. From exact analytical and numerical results, the complete phase diagram is presented. The phase…

Soft Condensed Matter · Physics 2009-11-10 Rajeev Kapri , Somendra M. Bhattacharjee , Flavio Seno

Using Monte Carlo simulations, we study the hysteresis in unzipping of a double stranded block copolymer DNA with $-A_n B_n-$ repeat units. Here $A$ and $B$ represent two different types of base pairs having two- and three-bonds,…

Soft Condensed Matter · Physics 2021-01-27 Ramu Kumar Yadav , Rajeev Kapri