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We analyse transcriptional bursting within a stochastic non-equilibrium model which accounts for the coupling between the dynamics of DNA supercoiling and gene transcription. We find a clear signature of bursty transcription when there is a…

Transcription regulation is largely governed by the profile and the dynamics of transcription factors' binding to DNA. Stochastic effects are intrinsic to this dynamics and the binding to functional sites must be controled with a certain…

Genomics · Quantitative Biology 2007-05-23 Erik Aurell , Aymeric Fouquier d'Herouel , Claes Malmnas , Massimo Vergassola

A theory for thermomechanical behavior of homogeneous DNA at thermal equilibrium predicts critical temperatures for denaturation under torque and stretch, phase diagrams for stable B--DNA, supercoiling, optimally stable torque, and the…

Soft Condensed Matter · Physics 2015-05-27 Cristiano Nisoli , A. R. Bishop

Literature data on the properties of DNA open states are reviewed and analyzed. These states are formed as a result of strong DNA fluctuations and have a great impact on a number of biochemical processes; among them is charge transfer in…

Biomolecules · Quantitative Biology 2020-02-04 A. S. Shigaev , O. A. Ponomarev , V. D. Lakhno

We investigate melting transition of DNA sequences embedded in a Langevin fluctuation-dissipation thermal bath. Torsional effects are considered by a twist angle $\varphi$ between neighboring base pairs stacked along the molecule backbone.…

Biomolecules · Quantitative Biology 2021-08-05 O. Farzadian , T. Oikonomou , M. Moradkhani

A dynamical mean-field theory is developed to analyze stochastic single-cell dynamics of gene expression. By explicitly taking account of nonequilibrium and nonadiabatic features of the DNA state fluctuation, two-time correlation functions…

Molecular Networks · Quantitative Biology 2009-11-13 Yurie Okabe , Yuu Yagi , Masaki Sasai

The dynamics of gene transcription is tightly regulated in eukaryotes. Recent experiments have revealed various kinds of transcriptional dynamics, such as RNA polymerase II pausing, that involves regulation at the transcription initiation…

Cell Behavior · Quantitative Biology 2015-08-11 Yucheng Hu , John Lowengrub

It has been speculated that bubble formation of several base-pairs due to thermal fluctuations is indicatory for biological active sites. Recent evidence, based on experiments and molecular dynamics (MD) simulations using the…

Biological Physics · Physics 2009-11-11 Titus S. van Erp , Santiago Cuesta-Lopez , Johannes-Geert Hagmann , Michel Peyrard

We study numerically the mechanical stability and elasticity properties of duplex DNA molecules within the frame of a network model incorporating microscopic degrees of freedom related with the arrangement of the base pairs. We pay special…

Pattern Formation and Solitons · Physics 2015-06-26 D. Hennig , J. F. R. Archilla

We theoretically investigated the temperature fluctuations of DNA close to denaturation and observed a strong enhancement of these fluctuations at the critical temperature. Although in a much lower frequency range, such a sharp increase was…

Biological Physics · Physics 2011-11-10 Marc Joyeux , Sahin Buyukdagli , Michael Sanrey

We have simulated structure and dynamics of water in the grooves of a DNA duplex using moleculear dynamics simulations. We find signatures of a dynamical transition in both translational and orientational dynamics of water molecules in both…

Soft Condensed Matter · Physics 2008-09-23 Debasmita Biswal , Biman Jana , Subrata Pal , Biman Bagchi

Statistical DNA models available in the literature are often effective models where the base-pair state only (unbroken or broken) is considered. Because of a decrease by a factor of 30 of the effective bending rigidity of a sequence of…

Soft Condensed Matter · Physics 2008-01-28 John Palmeri , Manoel Manghi , Nicolas Destainville

The force induced unzipping transition of a double stranded DNA is considered from a purely thermodynamic point of view. This analysis provides us with a set of relations that can be used to test microscopic theories and experiments. The…

Soft Condensed Matter · Physics 2015-06-18 Poulomi Sadhukhan , Somendra M. Bhattacharjee

We propose a model Hamiltonian for charge transfer along the DNA double helix with temperature driven fluctuations in the base pair positions acting as the rate limiting factor for charge transfer between neighboring base pairs. We compare…

Soft Condensed Matter · Physics 2009-10-31 R. Bruinsma , G. Gruner , M. R. D'Orsogna , J. Rudnick

We introduce a novel method to screen the promoters of a set of genes with shared biological function, against a precompiled library of motifs, and find those motifs which are statistically over-represented in the gene set. The gene sets…

Molecular Networks · Quantitative Biology 2008-11-11 Yuval Tabach , Ran Brosh , Yossi Buganim , Anat Reiner , Or Zuk , Assif Yitzhaky , Mark Koudritsky , Varda Rotter , Eytan Domany

The formation of DNA loops by proteins and protein complexes that bind at distal DNA sites plays a central role in many cellular processes, such as transcription, recombination, and replication. Here we review the basic thermodynamic…

Molecular Networks · Quantitative Biology 2007-05-23 Jose M. G. Vilar , Leonor Saiz

While the statistical mechanical description of DNA has a long tradition, renewed interest in DNA melting from a physics perspective is nourished by measurements of the fluctuation dynamics of local denaturation bubbles by single molecule…

Biomolecules · Quantitative Biology 2009-11-13 Tobias Ambjornsson , Suman K. Banik , Oleg Krichevsky , Ralf Metzler

The formation of bubbles in nucleic acids (NAs) are fundamental in many biological processes such as DNA replication, recombination, telomeres formation, nucleotide excision repair, as well as RNA transcription and splicing. These precesses…

Biomolecules · Quantitative Biology 2007-05-23 D. Hennig , J. F. R. Archilla , J. M. Romero

The free energy of inserting a protein into a membrane is determined by considering the variation in the spectrum of thermal fluctuations in response to the presence of a rigid inclusion. Both numerically and through a simple analytical…

Soft Condensed Matter · Physics 2007-05-23 Christian D. Santangelo , Oded Farago

Evolution of gene regulation is crucial for our understanding of the phenotypic differences between species, populations and individuals. Sequence-specific binding of transcription factors to the regulatory regions on the DNA is a key…

Populations and Evolution · Quantitative Biology 2015-11-09 Murat Tuğrul , Tiago Paixão , Nicholas H. Barton , Gašper Tkačik