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A proof is presented that gene regulatory networks (GRNs) based solely on transcription factors cannot control the development of complex multicellular life. GRNs alone cannot explain the evolution of multicellular life in the Cambrian…

Molecular Networks · Quantitative Biology 2014-01-28 Eric Werner

Transcriptional regulation of gene expression is one of the main processes that affect cell diversification from a single set of genes. Regulatory proteins often interact with DNA regions located distally from the transcription start sites…

Genomics · Quantitative Biology 2014-04-30 Sofie Demeyer , Tom Michoel

With the increasing amount of experimental data on gene expression and regulation, there is a growing need for quantitative models to describe the data and relate them to the different contexts. The thermodynamic models reviewed in the…

Molecular Networks · Quantitative Biology 2007-05-23 Lacramioara Bintu , Nicolas E. Buchler , Hernan G. Garcia , Ulrich Gerland , Terence Hwa , Jane' Kondev , Thomas Kuhlman , Rob Phillips

Cells are regulated by networks of controllers having many targets, and targets affected by many controllers, but these "many-to-many" combinatorial control systems are poorly understood. Here we analyze distinct cellular networks…

Molecular Networks · Quantitative Biology 2012-01-06 Jacob D. Feala , Jorge Cortes , Phillip M. Duxbury , Andrew D. McCulloch , Carlo Piermarocchi , Giovanni Paternostro

The transcriptional regulation of gene expression is orchestrated by complex networks of interacting genes. Increasing evidence indicates that these transcriptional regulatory networks (TRNs) in bacteria have an inherently hierarchical…

Molecular Networks · Quantitative Biology 2014-05-16 Santhust Kumar , Michele Vendruscolo , Amit Singh , Dhiraj Kumar , Areejit Samal

Oscillations lie at the core of many biological processes, from the cell cycle, to circadian oscillations and developmental processes. Time-keeping mechanisms are essential to enable organisms to adapt to varying conditions in environmental…

Machine Learning · Statistics 2015-04-27 D Trejo , AJ Millar , G Sanguinetti

Models of transcriptional regulation that assume equilibrium binding of transcription factors have been very successful at predicting gene expression from sequence in bacteria. However, analogous equilibrium models do not perform as well in…

Molecular Networks · Quantitative Biology 2021-10-14 Benjamin Zoller , Thomas Gregor , Gašper Tkačik

The dynamics of gene transcription is tightly regulated in eukaryotes. Recent experiments have revealed various kinds of transcriptional dynamics, such as RNA polymerase II pausing, that involves regulation at the transcription initiation…

Cell Behavior · Quantitative Biology 2015-08-11 Yucheng Hu , John Lowengrub

Motivation: One of the branches of Systems Biology is focused on a deep understanding of underlying regulatory networks through the analysis of the biomolecules oscillations and their interplay. Synthetic Biology exploits gene or/and…

Molecular Networks · Quantitative Biology 2020-11-30 Francesco Bardozzo , Pietro Liò , Roberto Tagliaferri

BACKGROUND: Transcriptional regulation is a key mechanism in the functioning of the cell, and is mostly effected through transcription factors binding to specific recognition motifs located upstream of the coding region of the regulated…

Genomics · Quantitative Biology 2007-05-23 Davide Cora' , Ferdinando Di Cunto , Paolo Provero , Lorenzo Silengo , Michele Caselle

Effective therapy of complex diseases requires control of highly non-linear complex networks that remain incompletely characterized. In particular, drug intervention can be seen as control of signaling in cellular networks. Identification…

Quantitative Methods · Quantitative Biology 2009-09-03 Jacob D. Feala , Jorge Cortes , Phillip M. Duxbury , Carlo Piermarocchi , Andrew D. McCulloch , Giovanni Paternostro

Increasing evidence suggests that chromosome folding and genetic expression are intimately connected. For example, the co-expression of a large number of genes can benefit from their spatial co-localization in the cellular space.…

Soft Condensed Matter · Physics 2018-03-13 Guillaume Le Treut

Transcriptional networks represent one of the most extensively studied types of systems in synthetic biology. Although the completeness of transcriptional networks for digital logic is well-established, *analog* computation plays a crucial…

Computational Complexity · Computer Science 2026-05-15 David Doty , Mina Latifi , David Soloveichick

Gene expression is controlled primarily by interactions between transcription factor proteins (TFs) and the regulatory DNA sequence, a process that can be captured well by thermodynamic models of regulation. These models, however, neglect…

Molecular Networks · Quantitative Biology 2015-12-16 Sarah A Cepeda-Humerez , Georg Rieckh , Gašper Tkačik

It is the goal of systems biology to understand the behavior of the whole in terms of the knowledge of the parts. This is hard to achieve in many cases due to the difficulty of characterizing the many constituents and their complex web of…

Molecular Networks · Quantitative Biology 2007-05-23 T. E. Kuhlman , Z. Zhang , M. H. Saier , T. Hwa

Gene regulatory networks constitute the first layer of the cellular computation for cell adaptation and surveillance. In these webs, a set of causal relations is built up from thousands of interactions between transcription factors and…

Molecular Networks · Quantitative Biology 2009-11-30 Carlos Rodriguez-Caso , Bernat Corominas-Murtra , Ricard V. Solé

Predicting how genetic variation affects phenotypic outcomes at the organismal, cellular, and molecular levels requires deciphering the cis-regulatory code, the sequence rules by which non-coding regions regulate genes. In this perspective,…

Engineering genetic networks to be both predictable and robust is a key challenge in synthetic biology. Synthetic circuits must reliably function in dynamic, stochastic and heterogeneous environments, and simple circuits can be studied to…

Quantitative Methods · Quantitative Biology 2015-06-30 Zach Hensel , Tatiana T. Marquez-Lago

Multiplex and multi-directional control of metabolic pathways is crucial for metabolic engineering to improve product yield of fuels, chemicals, and pharmaceuticals. To achieve this goal, artificial transcriptional regulators such as…

Biomolecules · Quantitative Biology 2017-04-12 Jiayuan Sheng , Weihua Guo , Christine Ash , Brendan Freitas , Mitchell Paoletti , Xueyang Feng

BACKGROUND. Signal recognition and information processing is a fundamental cellular function, which in part involves comprehensive transcriptional regulatory (TR) mechanisms carried out in response to complex environmental signals in the…

Molecular Networks · Quantitative Biology 2007-05-23 Illes J. Farkas , Chuang Wu , Chakra Chennubhotla , Ivet Bahar , Zoltan N. Oltvai