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Motivated by recent experiments on photon statistics from individual dye pairs planted on biomolecules and coupled by fluorescence resonance energy transfer (FRET), we show here that the FRET dynamics can be modelled by Gaussian random…

Quantum Physics · Physics 2016-09-28 G. O. Ariunbold , G. S. Agarwal , Z. Wang , H. Walther , M. O. Scully

We present an analytical model for Forster resonance energy transfer (FRET) between a donor and an acceptor placed in inhomogeneous and absorptive environment characterized by complex dielectric function, e.g., near a metal-dielectric…

Mesoscale and Nanoscale Physics · Physics 2025-04-21 L. S. Petrosyan , M. N. Noginov , T. V. Shahbazyan

Conformational dynamics of biomolecules are of fundamental importance for their function. Single-molecule F\"orster Resonance Energy Transfer (smFRET) is a powerful approach to inform on the structure and the dynamics of labeled molecules.…

F\"orster resonance energy transfer (FRET) is a quantum mechanical phenomenon involving the non-radiative transfer of energy between coupled electric dipoles. Due to the strong dependence of FRET on the distance between the dipoles, it is…

Chemical Physics · Physics 2024-12-04 David Frost , Keisha Cook , Hugo Sanabria

Fluorescence spectroscopy and modeling provide powerful means to characterize biomacromolecular structures, dynamics, and interactions. F\"orster resonance energy transfer serves as a key technique for this due to its nanometer-scale…

Biological Physics · Physics 2026-02-26 Thomas-Otavio Peulen , Daria Maksutova , Thorben Cordes

Recent advances in Fluorescence Resonance Energy Transfer (FRET) provides a way to measure and understand different biological systems and molecular interactions in nanometer order. In this report the introduction and principle of the FRET…

General Physics · Physics 2009-08-14 Syed Arshad Hussain

Spectral filtering of resonance fluorescence is widely employed to improve single photon purity and indistinguishability by removing unwanted backgrounds. For filter bandwidths approaching the emitter linewidth, complex behaviour is…

The applications of Fluorescence resonance energy transfer (FRET) have expanded tremendously in the last 25 years, and the technique has become a staple technique in many biological and biophysical fields. FRET can be used as spectroscopic…

Two different metrics are used to assess Forster resonance energy transfer (FRET) between fluorophores in the steady state: (1) acceptor-quenching of donor fluorescence, E (a.k.a. transfer efficiency); and (ii) donor-excited acceptor…

Chemical Physics · Physics 2009-11-13 Jeffrey T. Buboltz , Charles Bwalya , Santiago Reyes , Dobromir Kamburov

Single molecule FRET (fluorescence resonance energy transfer) is a powerful technique for detecting real-time conformational changes and molecular interactions during biological reactions. In this review, we examine different techniques of…

Biomolecules · Quantitative Biology 2007-05-23 Ivan Rasnik , Sean A. McKinney , Taekjip Ha

FRET measurements can provide dynamic spatial information on length scales smaller than the diffraction limit of light. Several methods exist to measure FRET between fluorophores, including Fluorescence Lifetime Imaging Microscopy (FLIM),…

Quantitative Methods · Quantitative Biology 2017-01-09 Bryan Kaye , Peter J. Foster , Tae Yeon Yoo , Daniel J. Needleman

Concomitant amplified spontaneous emission (ASE) and F\"orster resonance energy transfer (FRET) are investigated in electrospun light-emitting fibers. Upon dye-doping with a proper FRET couple system, free-standing fibrous mats exhibit…

A theory for the fluorescence resonance energy transfer (FRET) between a pair of semiconducting nanocrystal quantum dots is developed. Two types of donor-acceptor couplings for the FRET rate are described: dipole-dipole (d-d) and the…

Materials Science · Physics 2009-11-13 Roi Baer , Eran Rabani

In order to discern aggregation in solutions, we present a quantum mechanical analog of the photon statistics from fluorescent molecules diffusing through a focused beam. A generating functional is developed to fully describe the…

Biological Physics · Physics 2009-11-10 Hai-cang Ren , Noel L. Goddard , Gregoire Altan-Bonnet , Albert Libchaber

The electronic energy levels and optical transitions of a semiconductor quantum dot are subject to dynamics within the solid-state environment. In particular, fluctuating electric fields due to nearby charge traps or other quantum dots…

Mesoscale and Nanoscale Physics · Physics 2014-05-07 Clemens Matthiesen , Megan J. Stanley , Maxime Hugues , Edmund Clarke , Mete Atature

We have studied the influence of the local density of optical states (LDOS) on the rate and efficiency of Forster resonance energy transfer (FRET) from a donor to an acceptor. The donors and acceptors are dye molecules that are separated by…

Resonant energy transfers, i.e. the non-radiative redistribution of an electronic excitation between two particles coupled by the dipole-dipole interaction, lie at the heart of a variety of chemical and biological phenomena, most notably…

Real time laser induced fluorescence spectroscopy usage for microanalysis of DNA double helix defects is shown. The method is based on Forster resonance energy transfer (FRET) in intercalator-donor pair (acridine orange as a donor and…

An experimental strategy has been developed specifically for the study of composition-dependent phase behavior in multi-component artificial membranes. The strategy is based on steady-state measurements of fluorescence resonance energy…

Soft Condensed Matter · Physics 2007-08-14 Jeffrey T. Buboltz
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