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In this paper we review the applicability of autofluorescent proteins for single-molecule imaging in biology. The photophysical characteristics of several mutants of the Green Fluorescent Protein (GFP) and those of DsRed are compared and…

Optics · Physics 2007-05-23 Laurent Cognet , Françoise Coussen , Daniel Choquet , Brahim Lounis

Biological lasers have attracted vast attention because of their potential medical application prospects, especially the low threshold biological laser, which can be used for ultrasensitive biological detection while ensuring that its…

Optics · Physics 2022-06-29 Shuoying Zhao , Gaoshang Li , Xubiao Peng , Jiyang Ma , Zhangqi Yin , Qing Zhao

We demonstrate a novel all-optical switch consisting of two molecules: a primary fluorophore that can be switched between a fluorescent and a dark state by light of different wavelengths, and a secondary chromophore that facilitates…

Quantitative Methods · Quantitative Biology 2009-11-11 Mark Bates , Timothy R. Blosser , Xiaowei Zhuang

The coherent dynamics of vibronic wave packets in the green fluorescent protein is reported. At room temperature the non-stationary dynamics following impulsive photoexcitation displays an oscillating optical transmissivity pattern with…

Photonic crystal fibres (PCFs) are optical fibres that guide light using a modulated dielectric medium. They provide an exceptionally versatile platform for various applications, thanks to the flexibility with which light-guiding can be…

We employ heterodyne interferometry to investigate the effect of a single organic molecule on the phase of a propagating laser beam. We report on the first phase-contrast images of individual molecules and demonstrate a single-molecule…

Quantum Physics · Physics 2015-05-27 M. Pototschnig , Y. Chassagneux , J. Hwang , G. Zumofen , A. Renn , V. Sandoghdar

Controlling the light emitted by individual molecules is instrumental to a number of novel nanotechnologies ranging from super-resolution bio-imaging and molecular sensing to quantum nanophotonics. Molecular emission can be tailored by…

The green fluorescent protein (GFP) chromophore is an asymmetric monomethine dye system. In the resonance color theory of dyes, a strong optical excitation arises from interactions of two valence-bond structures with a third, higher…

Chemical Physics · Physics 2015-05-18 Seth Olsen , Ross H. McKenzie

We implemented a nanoelectronic interface between graphene field effect transistors (FETs) and soluble proteins. This enables production of bioelectronic devices that combine functionalities of the biomolecular and inorganic components. The…

Mesoscale and Nanoscale Physics · Physics 2015-06-03 Ye Lu , Mitchell B. Lerner , Zhengqing John Qi , Joseph J. Mitala , Jong Hsien Lim , Bohdana M. Discher , A. T. Charlie Johnson

All-optical switches are introduced which are based on deoxyribonucleic acid (DNA) in the form of electrospun fibers, where DNA is semi-intercalated with a push-pull, luminescent nonlinear pyrazoline derivative. Optical birefringence is…

Applied Physics · Physics 2018-12-18 Adam Szukalski , Maria Moffa , Andrea Camposeo , Dario Pisignano , Jaroslaw Mysliwiec

The soft character of organic materials leads to strong coupling between molecular nuclear and electronic dynamics. This coupling opens the way to control charge transport in organic electronic devices by inducing molecular vibrational…

The functional properties of photoactive proteins are governed by the interplay between bright and dark excited states. While the bright states are well-studied, the dark states, which are fundamental to photostability and light harvesting,…

The implementation of natural and artificial proteins with designer properties and functionalities offers unparalleled opportunity for functional nanoarchitectures formed through self-assembly. However, to exploit the opportunities offered…

We investigate the possibility of optical current control through single molecules which are weakly coupled to leads. A master equation approach for the transport through a molecule is combined with a Floquet theory for the time-dependent…

Chemical Physics · Physics 2016-09-08 Jörg Lehmann , Sébastien Camalet , Sigmund Kohler , Peter Hänggi

An Ising--like model of proteins is used to investigate the mechanical unfolding of the Green Fluorescent Protein along different directions. When the protein is pulled from its ends, we recover the major and minor unfolding pathways…

Soft Condensed Matter · Physics 2011-08-16 M. Caraglio , A. Imparato , A. Pelizzola

Identification and characterization of large protein complexes is a mainstay of biochemical toolboxes. Utilization of cross-linking chemicals can facilitate the capture and identification of transient or weak interactions of a transient…

Molecular Networks · Quantitative Biology 2016-06-15 Timothy D. Cummins , Gopal P. Sapkota

Topological photonic insulators pave the way toward efficient integrated photonic devices with minimized scattering losses. Optical properties of the majority of topological structures proposed to date are fixed by design such that no…

Optics · Physics 2019-02-01 Mikhail I. Shalaev , Wiktor Walasik , Natalia M. Litchinitser

In this letter, we analyze a genetic toggle switch recently studied in the literature where the expression of two repressor proteins can be tuned by controlling two different inputs, namely the concentration of two inducer molecules in the…

Molecular Networks · Quantitative Biology 2020-03-18 Davide Fiore , Agostino Guarino , Mario di Bernardo

A wide variety of feed gases are used to generate low-temperature plasmas for the microelectronics and the sustainability applications. These plasmas often have a complex combination of reactive and non-reactive species which may have…

We report the cell biological applications of a recently developed multiphoton fluorescence lifetime imaging microscopy system using a streak camera (StreakFLIM). The system was calibrated with standard fluorophore specimens and was shown…

Cell Behavior · Quantitative Biology 2009-11-10 R. V. Krishnan , Eva Biener , Jian-Hua Zhang , Robert Heckel , Brian Herman
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