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The characterisation of the physical properties of nanoparticles in their native environment plays a central role in a wide range of fields, from nanoparticle-enhanced drug delivery to environmental nanopollution assessment. Standard…

Label-free detecting multiple analytes in a high-throughput fashion has been one of the long-sought goals in biosensing applications. Yet, for all-optical approaches, interfacing state-of-the-art label-free techniques with microfluidics…

Quantitative Methods · Quantitative Biology 2023-08-14 Alexia Stollmann , Jose Garcia-Guirado , Jae-Sang Hong , Hyungsoon Im , Hakho Lee , Jaime Ortega Arroyo , Romain Quidant

Optical sensing is one of the key-enablers of modern diagnostics. Especially label-free imaging modalities hold great promise as they eliminate labeling procedures prior to analysis. However, scattering signals of nanometric particles scale…

Optics · Physics 2021-02-03 Unai Ortiz-Orruño , Ala Jo , Hakho Lee , Niek F. van Hulst , Matz Liebel

Droplet microfluidics offers a versatile platform for analyzing liquid samples. Despite its potential, there is a lack of techniques that allow to reliably probe individual circulating droplets. The prospect of combining droplet…

Quantitative Methods · Quantitative Biology 2024-03-08 Marc Sulliger , Jaime Ortega Arroyo , Romain Quidant

In order to relate nanoparticle properties to function, fast and detailed particle characterization, is needed. The ability to characterize nanoparticle samples using optical microscopy techniques has drastically improved over the past few…

Optical detection of individual nanometer-sized analytes, virus particles, and protein molecules holds great promise for understanding and control of biological samples and healthcare applications. As fluorescent labels impose restrictions…

Optics · Physics 2020-10-19 Martin Dieter Baaske , Peter Sebastian Neu , Michel Orrit

Single-molecule microscopy has become an indispensable tool for biochemical analysis. The capability of characterizing distinct properties of individual molecules without averaging has provided us with a different perspective for the…

Applied Physics · Physics 2020-05-21 Miao-Hsuan Chien , Silvan Schmid

Micro and nanoscale particles are crucial in various fields, from biomedical imaging to environmental processes. While conventional spectroscopy and microscopy methods for characterizing these particles often involve bulky equipment and…

Biological Physics · Physics 2024-11-26 Jie Liao , Maxwell Adolphson , Hangyue Li , Dipayon Kumar Sikder , Chenyang Lu , Lan Yang

The photo-kinetics of fluorescent molecules have enabled the circumvention of far-field optical diffraction-limit. Despite its enormous potential, the necessity to label the sample may adversely influence the delicate biology under…

Label-free single-cell imaging offers a scalable, non-invasive alternative to fluorescence-based cytometry, yet inferring molecular phenotypes directly from bright-field morphology remains challenging. We present a unified Deep Learning…

Computer Vision and Pattern Recognition · Computer Science 2026-05-15 Saqib Nazir , Ardhendu Behera

Despite its potential for label-free particle diagnostics, holographic microscopy is limited by specialized processing methods that struggle to generalize across diverse settings. We introduce a deep learning architecture leveraging human…

Optics · Physics 2024-11-26 Shyam Kumar , Jiarong Hong

We introduce HAMscope, a compact, snapshot hyperspectral autofluorescence miniscope that enables real-time, label-free molecular imaging in a wide range of biological systems. By integrating a thin polymer diffuser into a widefield…

Plastic nanoparticles present technological opportunities and environmental concerns, but measurement challenges impede product development and hazard assessment. To meet these challenges, we advance a lateral nanoflow assay that integrates…

Soft Condensed Matter · Physics 2022-05-19 Kuo-Tang Liao , Andrew C. Madison , Adam L. Pintar , B. Robert Ilic , Craig R. Copeland , Samuel M. Stavis

High-speed tracking of single particles is a gateway to understanding physical, chemical, and biological processes at the nanoscale. It is also a major experimental challenge, particularly for small, nanometer-scale particles. Although…

High-Q optical resonators allow label-free detection of individual nanoparticles through perturbation of optical signatures but have practical limitations due to reliance on random diffusion to deliver particles to the sensing region. We…

Optics · Physics 2016-10-03 Jeewon Suh , Kewen Han , Christopher W Peterson , Gaurav Bahl

High-throughput label-free measurements of the optical and mechanical properties of single microparticles play an important role in biological research, drug development, and related large population assays. Mechanical detection techniques…

Instrumentation and Detectors · Physics 2018-12-05 Kewen Han , Jeewon Suh , Gaurav Bahl

The development of robust, real-time optical methods for the detection and tracking of particles in complex multiple scattering media is a problem of practical importance in a number of fields, including environmental monitoring, air…

Optics · Physics 2025-02-04 Mohammadrasoul Taghavi , Edwin A. Marengo

Holographic displays promise several benefits including high quality 3D imagery, accurate accommodation cues, and compact form-factors. However, holography relies on coherent illumination which can create undesirable speckle noise in the…

Graphics · Computer Science 2023-09-20 Grace Kuo , Florian Schiffers , Douglas Lanman , Oliver Cossairt , Nathan Matsuda

Screening of unknown particles, including viruses and nanoparticles, is key in medicine, industry and pollutant determination. However, existing techniques require sample a priori knowledge or modification (e.g. fluorescence). Here we…

Laser speckle, the granular intensity pattern arising from random optical interference, provides a high-dimensional encoding of spectral information that can be exploited for precision metrology. Speckle-based spectrometers have advanced…

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