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We have implemented a multi-functional optofluidic sensor that can monitor changes in the refractive index and pressure of biofluid simultaneously and can detect free-solution molecular interaction in-situ. In this paper, we demonstrate two…

Optics · Physics 2014-10-16 Zhida Xu , Logan Liu

Fluorescence detection, either involving propagating or near-field emission, is widely being used in spectroscopy, sensing and microscopy. Total internal reflection fluorescence (TIRF) confines fluorescence excitation by an evanescent…

Quantitative Methods · Quantitative Biology 2020-06-24 Martin Oheim , Adi Salomon , Maia Brunstein

We present a theoretical study of the directionality effects in spontaneous emission and resonance fluorescence of a quantum two-level dipole emitter near an ultrathin closely packed periodically aligned single-wall carbon nanotube film.…

Mesoscale and Nanoscale Physics · Physics 2024-10-15 Michael D. Pugh , SK Firoz Islam , Igor V. Bondarev

High-throughput label-free measurements of the optical and mechanical properties of single microparticles play an important role in biological research, drug development, and related large population assays. Mechanical detection techniques…

Instrumentation and Detectors · Physics 2018-12-05 Kewen Han , Jeewon Suh , Gaurav Bahl

Pixel size in cameras and other refractive imaging devices is typically limited by the free-space diffraction. However, a vast majority of semiconductor-based detectors are based on materials with substantially high refractive index. We…

Optics · Physics 2018-03-28 Bo Fan , Sandeep Inampudi , Viktor A. Podolskiy

We demonstrate chip-scale flat-top filters at near-infrared wavelengths using negative index photonic crystal based Mach Zehnder interferometers. Supported by full three-dimensional numerical simulations, we experimentally demonstrate a new…

Optics · Physics 2015-06-03 S. Kocaman , M. S. Aras , N. C. Panoiu , M. Lu , C. W. Wong

Interferometric scattering (iSCAT) microscopy is a powerful tool for label-free sensitive detection and imaging of nanoparticles to high spatio-temporal resolution. As it was born out of detection principles central to conventional…

Optics · Physics 2018-12-31 Richard W. Taylor , Vahid Sandoghdar

The detection of spatial or temporal variations in very thin samples has important applications in the biological sciences. For example, cellular membranes exhibit changes in lipid composition and order, which in turn modulate their…

Photonic integrated circuit devices can be used as refractometric opto-fluidic sensors to detect the presence of analytes in solution at low concentrations. In this work, we investigate the refractive index sensitivity of silicon nitride…

Adaptive optics in combination with multi-photon techniques is a powerful approach to image deep into a specimen. Remarkably, virtually all adaptive optics schemes today rely on wavefront modulators which are reflective, diffractive, or…

Three-dimensional (3D) imaging of the subcellular organisation and morphology of cells and tissues is essential for understanding biological function. Although staining is the most widely used approach for visualising biological samples…

Reflection phase imaging provides label-free, high-resolution characterization of biological samples, typically using interferometric-based techniques. Here, we investigate reflection phase microscopy from intensity-only measurements under…

Quantitative Methods · Quantitative Biology 2019-12-18 Alex Matlock , Anne Sentenac , Patrick C. Chaumet , Ji Yi , Lei Tian

Interferometric scattering microscopy has been a very promising technology for highly sensitive label-free imaging of a broad spectrum of biological nanoparticles from proteins to viruses in a high-throughput manner. Although it can reveal…

Optics · Physics 2021-08-11 Celalettin Yurdakul , Haonan Zong , Yeran Bai , Ji-Xin Cheng , M. Selim Unlu

Non-invasive morphological assessment is the cornerstone of oocyte and embryo selection in assisted reproductive technology, yet clinical practice remains limited by two-dimensional, qualitative microscopy. While three-dimensional (3D)…

Reciprocity breaking at optical frequencies typically relies on bulky magnets, dynamic modulation, or nonlinearities, all of which hinder chip-scale integration and the handling of unpolarised light. We introduce a fully passive,…

Optical detection of individual nanometer-sized analytes, virus particles, and protein molecules holds great promise for understanding and control of biological samples and healthcare applications. As fluorescent labels impose restrictions…

Optics · Physics 2020-10-19 Martin Dieter Baaske , Peter Sebastian Neu , Michel Orrit

A simple method was developed to observe the interference patterns of the light reflected by the interfaces of thin liquid films. Employing a fluorescent microscope with epi-illumination, we collected the 2D patterns of interference fringes…

Optics · Physics 2010-06-14 V. Berejnov , D. Li

The electronic functionality of thin films is governed by their interfaces. This is very important for the ferroelectric (FE) state which depends on thin-film clamping and interfacial charge transfer. Here we show that in a heterostructure…

Mesoscale and Nanoscale Physics · Physics 2020-02-24 Michael Huth , Achim Rippert , Roland Sachser , Lukas Keller

Our ability to optically interrogate nanoscopic objects is controlled by the difference between their extinction cross sections and the diffraction limited area to which light can be confined in the far field. We show that a partially…

Optics · Physics 2016-11-17 Daniel Cole , Gavin Young , Alexander Weigel , P. Kukura

Featured with a plethora of electric and magnetic Mie resonances, high index dielectric nanostructures offer a versatile platform to concentrate light-matter interactions at the nanoscale. By integrating unique features of far-field…