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Hill functions, dominant in gene regulatory network modeling, carry fundamental limitations: at non-integer cooperativity exponents, routine when fitting dose-response data, derivatives diverge at the origin, complex arithmetic corrupts ODE…

Dynamical Systems · Mathematics 2026-04-30 Ismail Belgacem

Hill functions dominate gene regulatory network (GRN) modeling, but their fractional exponents create analytical pathologies when the Hill coefficient $n$ is non-integer -- a ubiquitous occurrence in experimental fits. We replace the Hill…

Dynamical Systems · Mathematics 2026-05-06 Ismail Belgacem

The logistic two-gene negative-feedback oscillator is locally asymptotically stable for all biological parameter values, since the trace of the Jacobian is uniformly negative. Real biological oscillators (circadian rhythms, the segmentation…

Dynamical Systems · Mathematics 2026-05-25 Ismail Belgacem

Gene regulatory networks are typically modeled using ordinary differential equations (ODEs) with phenomenological Hill functions to represent transcriptional regulation. While computationally efficient, Hill functions lack mechanistic…

Molecular Networks · Quantitative Biology 2026-01-16 Tianyu Wu

Deterministic and stochastic Boolean network models are build for the dynamics of negative feedback loops of the p53 pathways. It is shown that the main function of the negative feedback in the p53 pathways is to keep p53 at a low steady…

Molecular Networks · Quantitative Biology 2009-04-16 Hao Ge , Min Qian

Binarization of gene expression data is a \textbf{critical prerequisite} for the synthesis of Boolean gene regulatory network (GRN) models from omics datasets. Because Boolean networks encode gene activity as binary variables, the accuracy…

Discrete Mathematics · Computer Science 2025-10-21 Ismail Belgacem , Franck Delaplace

In this paper, we study the stability of an Ordinary Differential Equation (ODE) usually referred to as Cyclic Feedback Loop, which typically models a biological network of molecules where each molecule regulates its successor in a cycle.…

Classical Analysis and ODEs · Mathematics 2023-04-26 Jules Guilberteau

Gene regulatory networks, i.e. DNA segments in a cell which interact with each other indirectly through their RNA and protein products, lie at the heart of many important intracellular signal transduction processes. In this paper we analyse…

Analysis of PDEs · Mathematics 2014-04-03 Mark Chaplain , Mariya Ptashnyk , Marc Sturrock

The global dynamics of gene regulatory networks are known to show robustness to perturbations in the form of intrinsic and extrinsic noise, as well as mutations of individual genes. One molecular mechanism underlying this robustness has…

Molecular Networks · Quantitative Biology 2015-06-15 Claus Kadelka , David Murrugarra , Reinhard Laubenbacher

The complex dynamics of gene expression in living cells can be well-approximated using Boolean networks. The average sensitivity is a natural measure of stability in these systems: values below one indicate typically stable dynamics…

Molecular Networks · Quantitative Biology 2018-10-03 Bryan C. Daniels , Hyunju Kim , Douglas Moore , Siyu Zhou , Harrison Smith , Bradley Karas , Stuart A. Kauffman , Sara I. Walker

Coregulation of the expression of groups of genes has been extensively demonstrated empirically in bacterial and eukaryotic systems. Such coregulation can arise through the use of shared regulatory motifs, which allow the coordinated…

Machine Learning · Statistics 2016-04-12 Jonathan H. Warrell , Musa M. Mhlanga

Describing reaction rates in stochastic bio-circuits is commonly done by directly introducing the deterministically deduced Hill function into the master equation. However, when fluctuations in enzymatic reaction rates are not neglectable,…

Molecular Networks · Quantitative Biology 2024-08-23 Manuel Eduardo Hernández-García , Jorge Velázquez-Castro

Living things benefit from exquisite molecular sensitivity in many of their key processes, including DNA replication, transcription and translation, chemical sensing, and morphogenesis. At thermodynamic equilibrium, the basic biophysical…

Statistical Mechanics · Physics 2021-12-16 Jeremy A. Owen , Jordan M. Horowitz

A great part of the effort in the study of coarse grained models of transcription networks is directed to the analysis of their dynamical features. In this letter, we consider the \emph{equilibrium} properties of such systems, showing that…

Molecular Networks · Quantitative Biology 2007-05-23 M. Cosentino Lagomarsino , P. Jona , B. Bassetti

ODE-based models for gene regulatory networks (GRNs) can often be formulated as smooth singular perturbation problems with multiple small parameters, some of which are related to time-scale separation, whereas others are related to…

Dynamical Systems · Mathematics 2025-10-09 Samuel Jelbart , Kristian Uldall Kristiansen , Peter Szmolyan

We present an approach for constructing dynamic models for the simulation of gene regulatory networks from simple computational elements. Each element is called a ``gene gate'' and defines an input/output-relationship corresponding to the…

Molecular Networks · Quantitative Biology 2007-10-09 R. Blossey , L. Cardelli , A. Phillips

We consider a model of large regulatory gene expression networks where the thresholds activating the sigmoidal interactions between genes and the signs of these interactions are shuffled randomly. Such an approach allows for a qualitative…

Molecular Networks · Quantitative Biology 2007-05-23 D. Volchenkov , R. Lima

Statistical solutions of incompressible Euler describe turbulent dynamics as time-parameterized laws on $L^2$ whose multi-point correlations satisfy an infinite hierarchy of weak identities. Modern generative samplers for PDE forecasting…

Analysis of PDEs · Mathematics 2026-02-24 Victor Armegioiu

Hill function is one of the widely used gene transcription regulation models. Its attribute of fitting may result in a lack of an underlying physical picture, yet the fitting parameters can provide information about biochemical reactions,…

Molecular Networks · Quantitative Biology 2024-03-05 Wenjia Shi , Yao Ma , Peilin Hu , Mi Pang , Xiaona Huang , Yiting Dang , Yuxin Xie , Danni Wu

We prove that nested canalizing functions are the minimum-sensitivity Boolean functions for any activity ratio and we determine the functional form of this boundary which has a nontrivial fractal structure. We further observe that the…

Molecular Networks · Quantitative Biology 2024-08-14 Hamza Coban
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