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We present a weakly supervised deep learning method to perform instance segmentation of cells present in microscopy images. Annotation of biomedical images in the lab can be scarce, incomplete, and inaccurate. This is of concern when…

Computer Vision and Pattern Recognition · Computer Science 2019-08-28 Fidel A. Guerrero-Peña , Pedro D. Marrero Fernandez , Tsang Ing Ren , Alexandre Cunha

The spatial location of cells within tissues and organs is crucial for the manifestation of their specific functions.Spatial transcriptomics technology enables comprehensive measurement of the gene expression patterns in tissues while…

Quantitative Methods · Quantitative Biology 2024-07-12 Shuailin Xue , Fangfang Zhu , Changmiao Wang , Wenwen Min

Spatial transcriptomics (ST) technologies enable gene expression profiling with spatial resolution, offering unprecedented insights into tissue organization and disease heterogeneity. However, current analysis methods often struggle with…

Spatial transcriptomics (ST) is essential for understanding diseases and developing novel treatments. It measures gene expression of each fine-grained area (i.e., different windows) in the tissue slide with low throughput. This paper…

Computer Vision and Pattern Recognition · Computer Science 2022-11-01 Yan Yang , Md Zakir Hossain , Eric A Stone , Shafin Rahman

The need for labour intensive pixel-wise annotation is a major limitation of many fully supervised learning methods for segmenting bioimages that can contain numerous object instances with thin separations. In this paper, we introduce a…

Computer Vision and Pattern Recognition · Computer Science 2020-09-11 Rihuan Ke , Aurélie Bugeau , Nicolas Papadakis , Peter Schuetz , Carola-Bibiane Schönlieb

Digital pathology is one of the most significant developments in modern medicine. Pathological examinations are the gold standard of medical protocols and play a fundamental role in diagnosis. Recently, with the advent of digital scanners,…

Image and Video Processing · Electrical Eng. & Systems 2021-12-09 Mahdi Arab Loodaricheh , Nader Karimi , Shadrokh Samavi

In recent years, deep learning (DL) methods have become powerful tools for biomedical image segmentation. However, high annotation efforts and costs are commonly needed to acquire sufficient biomedical training data for DL models. To…

Computer Vision and Pattern Recognition · Computer Science 2018-06-05 Lin Yang , Yizhe Zhang , Zhuo Zhao , Hao Zheng , Peixian Liang , Michael T. C. Ying , Anil T. Ahuja , Danny Z. Chen

Multi-class cell segmentation in high-resolution Giga-pixel whole slide images (WSI) is critical for various clinical applications. Training such an AI model typically requires labor-intensive pixel-wise manual annotation from experienced…

Nuclear segmentation in histology images is a challenging task due to significant variations in the shape and appearance of nuclei. One of the main hurdles in nuclear instance segmentation is overlapping nuclei where a smart algorithm is…

Image and Video Processing · Electrical Eng. & Systems 2019-08-28 Navid Alemi Koohbanani , Mostafa Jahanifar , Ali Gooya , Nasir Rajpoot

Cellular identity and function are linked to both their intrinsic genomic makeup and extrinsic spatial context within the tissue microenvironment. Spatial transcriptomics (ST) offers an unprecedented opportunity to study this, providing in…

Machine Learning · Computer Science 2026-02-16 Rui Yan , Xiaohan Xing , Xun Wang , Zixia Zhou , Md Tauhidul Islam , Lei Xing

Spatial transcriptomics (ST) is a novel technique that simultaneously captures pathological images and gene expression profiling with spatial coordinates. Since ST is closely related to pathological features such as disease subtypes, it may…

Computer Vision and Pattern Recognition · Computer Science 2025-03-11 Kazuya Nishimura , Ryoma Bise , Yasuhiro Kojima

Histopathology nuclei segmentation is crucial for quantitative tissue analysis and cancer diagnosis. Although existing segmentation methods have achieved strong performance, they are often computationally heavy and show limited…

Computer Vision and Pattern Recognition · Computer Science 2026-03-24 Muhammad Hassan Maqsood , Yanming Zhu , Alfred Lam , Getamesay Dagnaw , Xuefei Yin , Alan Wee-Chung Liew

Despite the remarkable performance of deep learning methods on various tasks, most cutting-edge models rely heavily on large-scale annotated training examples, which are often unavailable for clinical and health care tasks. The labeling…

Computer Vision and Pattern Recognition · Computer Science 2021-03-02 Jialin Peng , Ye Wang

Semantic segmentation is the task of classifying each pixel in an image. Training a segmentation model achieves best results using annotated images, where each pixel is annotated with the corresponding class. When obtaining fine annotations…

Computer Vision and Pattern Recognition · Computer Science 2025-10-20 Jort de Jong , Mike Holenderski

Nuclei segmentation is a fundamental but challenging task in the quantitative analysis of histopathology images. Although fully-supervised deep learning-based methods have made significant progress, a large number of labeled images are…

Image and Video Processing · Electrical Eng. & Systems 2024-01-22 Xinyi Yu , Guanbin Li , Wei Lou , Siqi Liu , Xiang Wan , Yan Chen , Haofeng Li

Current deep learning-based approaches for the segmentation of microscopy images heavily rely on large amount of training data with dense annotation, which is highly costly and laborious in practice. Compared to full annotation where the…

Computer Vision and Pattern Recognition · Computer Science 2023-12-11 Shijie Li , Mengwei Ren , Thomas Ach , Guido Gerig

Deep Learning (DL) models have been successfully applied to many applications including biomedical cell segmentation and classification in histological images. These models require large amounts of annotated data which might not always be…

Image and Video Processing · Electrical Eng. & Systems 2024-06-04 Roberto Basla , Loris Giulivi , Luca Magri , Giacomo Boracchi

Image segmentation is a fundamental problem in medical image analysis. In recent years, deep neural networks achieve impressive performances on many medical image segmentation tasks by supervised learning on large manually annotated data.…

Computer Vision and Pattern Recognition · Computer Science 2018-01-26 Ling Zhang , Vissagan Gopalakrishnan , Le Lu , Ronald M. Summers , Joel Moss , Jianhua Yao

Deep Brain Stimulation (DBS) is one of the most successful methods to diminish late-stage Parkinson's Disease (PD) symptoms. It is a delicate surgical procedure which requires detailed pre-surgical patient's study. High-field Magnetic…

Image and Video Processing · Electrical Eng. & Systems 2024-07-23 Tomás Lima , Igor Varga , Eduard Bakštein , Daniel Novák , Victor Alves

Deep convolutional neural networks (CNNs) have shown excellent performance in object recognition tasks and dense classification problems such as semantic segmentation. However, training deep neural networks on large and sparse datasets is…

Computer Vision and Pattern Recognition · Computer Science 2017-12-25 Lorenz Berger , Eoin Hyde , M. Jorge Cardoso , Sebastien Ourselin