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Fluorescence microscopy, while being a key driver for progress in the life sciences, is also subject to technical limitations. To overcome them, computational multiplexing techniques have recently been proposed, which allow multiple…

Computer Vision and Pattern Recognition · Computer Science 2025-10-22 Ashesh Ashesh , Florian Jug

Spectral unmixing is an important task in hyperspectral image processing for separating the mixed spectral data pertaining to various materials observed individual pixels. Recently, nonlinear spectral unmixing has received particular…

Image and Video Processing · Electrical Eng. & Systems 2021-10-07 Min Zhao , Mou Wang , Jie Chen , Susanto Rahardja

The development of signal unmixing algorithms is essential for leveraging multimodal datasets acquired through a wide array of scientific imaging technologies, including hyperspectral or time-resolved acquisitions. In experimental physics,…

In this work, we present denoiSplit, a method to tackle a new analysis task, i.e. the challenge of joint semantic image splitting and unsupervised denoising. This dual approach has important applications in fluorescence microscopy, where…

Image and Video Processing · Electrical Eng. & Systems 2024-08-13 Ashesh Ashesh , Florian Jug

Hyperspectral unmixing, the process of estimating a common set of spectral bases and their corresponding composite percentages at each pixel, is an important task for hyperspectral analysis, visualization and understanding. From an…

Computer Vision and Pattern Recognition · Computer Science 2014-11-18 Feiyun Zhu , Ying Wang , Bin Fan , Gaofeng Meng , Shiming Xiang , Chunhong Pan

Hyperspectral (HS) unmixing is the process of decomposing an HS image into material-specific spectra (endmembers) and their spatial distributions (abundance maps). Existing unmixing methods have two limitations with respect to noise…

Image and Video Processing · Electrical Eng. & Systems 2024-03-20 Kazuki Naganuma , Shunsuke Ono

We present {\mu}Split, a dedicated approach for trained image decomposition in the context of fluorescence microscopy images. We find that best results using regular deep architectures are achieved when large image patches are used during…

Computer Vision and Pattern Recognition · Computer Science 2023-08-21 Ashesh , Alexander Krull , Moises Di Sante , Francesco Silvio Pasqualini , Florian Jug

Hyperspectral Imaging (HSI) for fluorescence-guided brain tumor resection enables visualization of differences between tissues that are not distinguishable to humans. This augmentation can maximize brain tumor resection, improving patient…

Image and Video Processing · Electrical Eng. & Systems 2024-02-07 David Black , Jaidev Gill , Andrew Xie , Benoit Liquet , Antonio Di leva , Walter Stummer , Eric Suero Molina

Raman spectroscopy is widely used across scientific domains to characterize the chemical composition of samples in a non-destructive, label-free manner. Many applications entail the unmixing of signals from mixtures of molecular species to…

Hyperspectral unmixing is the process of determining the presence of individual materials and their respective abundances from an observed pixel spectrum. Unmixing is a fundamental process in hyperspectral image analysis, and is growing in…

Image and Video Processing · Electrical Eng. & Systems 2024-08-15 Jade Preston , William Basener

Imaging spectrometers measure electromagnetic energy scattered in their instantaneous field view in hundreds or thousands of spectral channels with higher spectral resolution than multispectral cameras. Imaging spectrometers are therefore…

Data Analysis, Statistics and Probability · Physics 2012-04-25 José M. Bioucas-Dias , Antonio Plaza , Nicolas Dobigeon , Mario Parente , Qian Du , Paul Gader , Jocelyn Chanussot

The direct detection of exoplanets with high-contrast instruments can be boosted with high spectral resolution. For integral field spectrographs yielding hyperspectral data, this means that the field of view consists of diffracted starlight…

Instrumentation and Methods for Astrophysics · Physics 2021-06-09 Julien Rameau , Jocelyn Chanussot , Alexis Carlotti , Mickael Bonnefoy , Philippe Delorme

Unmixing is a fundamental process in hyperspectral image processing in which the materials present in a mixed pixel are determined based on the spectra of candidate materials and the pixel spectrum. Practical and general utility requires a…

Image and Video Processing · Electrical Eng. & Systems 2025-03-24 Jade Preston , William Basener

Machine learning has achieved impressive performance in tomographic reconstruction, but supervised training requires paired measurements and ground-truth images that are often unavailable. This has motivated self-supervised approaches,…

Computer Vision and Pattern Recognition · Computer Science 2026-04-20 Markus Haltmeier , Lukas Neumann , Nadja Gruber , Gyeongha Hwang

Single-pixel imaging has emerged as a key technique in fluorescence microscopy, where fast acquisition and reconstruction are crucial. In this context, images are reconstructed from linearly compressed measurements. In practice, total…

Image and Video Processing · Electrical Eng. & Systems 2025-07-28 Serban C. Tudosie , Valerio Gandolfi , Shivaprasad Varakkoth , Andrea Farina , Cosimo D'Andrea , Simon Arridge

Linear spectral unmixing is an essential technique in hyperspectral image processing and interpretation. In recent years, deep learning-based approaches have shown great promise in hyperspectral unmixing, in particular, unsupervised…

Image and Video Processing · Electrical Eng. & Systems 2022-08-10 Lin Qi , Feng Gao , Junyu Dong , Xinbo Gao , Qian Du

Deep learning based unmixing methods have received great attention in recent years and achieve remarkable performance. These methods employ a data-driven approach to extract structure features from hyperspectral image, however, they tend to…

Image and Video Processing · Electrical Eng. & Systems 2024-09-10 Min Zhao , Linruize Tang , Jie Chen

One of the challenges in hyperspectral data analysis is the presence of mixed pixels. Mixed pixels are the result of low spatial resolution of hyperspectral sensors. Spectral unmixing methods decompose a mixed pixel into a set of endmembers…

Computer Vision and Pattern Recognition · Computer Science 2015-06-05 Roozbeh Rajabi , Hassan Ghassemian

Spectral variability is one of the major issue when conducting hyperspectral unmixing. Within a given image composed of some elementary materials (herein referred to as endmember classes), the spectral signature characterizing these classes…

Image and Video Processing · Electrical Eng. & Systems 2019-06-26 Tatsumi Uezato , Mathieu Fauvel , Nicolas Dobigeon

In the remote sensing context spectral unmixing is a technique to decompose a mixed pixel into two fundamental representatives: endmembers and abundances. In this paper, a novel architecture is proposed to perform blind unmixing on…

Computer Vision and Pattern Recognition · Computer Science 2020-11-19 Yasiru Ranasinghe , Sanjaya Herath , Kavinga Weerasooriya , Mevan Ekanayake , Roshan Godaliyadda , Parakrama Ekanayake , Vijitha Herath
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