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Digital holographic microscopy (DHM) applied to quantitative phase imaging (QPI) has been successfully demonstrated as a powerful label-free method to analyse the optical properties of cells. Spatially multiplexed interferometric microscopy…

Scanning ion conductance microscopy (SICM) can image the surface topography of specimens in ionic solutions without mechanical probe--sample contact. This unique capability is advantageous for imaging fragile biological samples but its…

Applied Physics · Physics 2019-12-25 Shinji Watanabe , Satoko Kitazawa , Linhao Sun , Noriyuki Kodera , Toshio Ando

We report the modification of a label-free image scanning microscope (ISM) to perform asynchronous 2D imaging at 24kHz while keeping the lateral resolution gain and background rejection of a regular label-free ISM setup. Our method uses a…

Optics · Physics 2023-08-31 Duc-Minh Ta , Alberto Aguilar , Pierre Bon

Multiple-input multiple-output (MIMO) orthogonal frequency-division multiplexing (OFDM) systems rely on digital or hybrid digital and analog designs for beamforming against frequency-selective fading, which suffer from high hardware…

Signal Processing · Electrical Eng. & Systems 2026-01-26 Zheao Li , Jiancheng An , Chau Yuen

In fluid flow imaging, intensity gradients are a good measure of spatial variations in scalar properties, which play an important role in controlling transport processes. However, current flow imaging techniques exhibit system-limited…

Optics · Physics 2025-10-22 Hy Cao , Abhishek Saha , Lisa V. Poulikakos

Photoacoustic microscopy (PAM) is a novel implementation of photoacoustic imaging (PAI) for visualizing the 3D bio-structure, which is realized by raster scanning of the tissue. However, as three involved critical imaging parameters,…

Image and Video Processing · Electrical Eng. & Systems 2023-08-10 Zhengyuan Zhang , Haoran Jin , Zesheng Zheng , Wenwen Zhang , Wenhao Lu , Feng Qin , Arunima Sharma , Manojit Pramanik , Yuanjin Zheng

Microscopy is routinely used to image biological structures of interest. Due to imaging constraints, acquired images, also called as micrographs, are typically low-SNR and contain noise. Over the last few years, regression-based tasks like…

Image and Video Processing · Electrical Eng. & Systems 2024-10-16 Ashesh Ashesh , Joran Deschamps , Florian Jug

Biological and biomedical samples are routinely examined using focused two-photon (2P) fluorescence microscopy due to its intrinsic axial sectioning and reduced out-of-focus bleaching. However, 2P imaging often requires excitation…

A real-time image reconstruction method for scanning transmission electron microscopy (STEM) is proposed. With an algorithm requiring only the center of mass (COM) of the diffraction pattern at one probe position at a time, it is able to…

Materials Science · Physics 2021-12-15 Chu-Ping Yu , Thomas Friedrich , Daen Jannis , Sandra Van Aert , Johan Verbeeck

By integrating a phase-only Spatial Light Modulator (SLM) into the illumination arm of a cylindrical-lens-based Selective Plane Illumination Microscope (SPIM), we have created a versatile system able to deliver high quality images by…

Biological Physics · Physics 2018-08-10 Chiara Garbellotto , Jonathan M. Taylor

We present a new two-snapshot structured light illumination (SLI) reconstruction algorithm for fast image acquisition. The new algorithm, which only requires two mutually {\pi} phase-shifted raw structured images, is implemented on a…

Optics · Physics 2017-10-25 Yunlong Meng , Wei Lin , Chenglin Li , Shih-chi Chen

We present a computational method for full-range interferometric synthetic aperture microscopy (ISAM) under dispersion encoding. With this, one can effectively double the depth range of optical coherence tomography (OCT), whilst…

Image and Video Processing · Electrical Eng. & Systems 2020-02-03 Jonathan H. Mason , Mike E. Davies , Pierre O. Bagnaninchi

Quantitative phase imaging (QPI) has been widely applied in characterizing cells and tissues. Spatial light interference microscopy (SLIM) is a highly sensitive QPI method, due to its partially coherent illumination and common path…

Image and Video Processing · Electrical Eng. & Systems 2024-06-12 Yuheng Jiao , Yuchen R. He , Mikhail E. Kandel , Xiaojun Liu , Wenlong Lu , Gabriel Popescu

Leveraging the multilayer realization of programmable metasurfaces, stacked intelligent metasurfaces (SIM) enable fine-grained wave-domain control. However, their wideband deployment is impeded by two structural factors: (i) a single,…

Signal Processing · Electrical Eng. & Systems 2026-03-16 Zheao Li , Jiancheng An , Chau Yuen

Confocal laser-scanning microscopy (CLSM) is one of the most popular optical architectures for fluorescence imaging. In CLSM, a focused laser beam excites the fluorescence emission from a specific specimen position. Some actuators scan the…

Image and Video Processing · Electrical Eng. & Systems 2023-05-10 Alessandro Zunino , Marco Castello , Giuseppe Vicidomini

4D live fluorescence microscopy is often compromised by prolonged high intensity illumination which induces photobleaching and phototoxic effects that generate photo-induced artifacts and severely impair image continuity and detail…

Image and Video Processing · Electrical Eng. & Systems 2026-02-17 Cunmin Zhao , Ziyuan Luo , Guoye Guan , Zelin Li , Yiming Ma , Zhongying Zhao , Renjie Wan

This retrospective-prospective study evaluated whether a deep learning-based MRI reconstruction algorithm can preserve diagnostic quality in brain MRI scans accelerated up to fourfold, using both public and prospective clinical data. The…

Image and Video Processing · Electrical Eng. & Systems 2025-09-10 Jonathan I. Mandel , Shivaprakash Hiremath , Hedyeh Keshtgar , Timothy Scholl , Sadegh Raeisi

We report a new coherent imaging technique, termed ptychographic structured modulation (PSM), for quantitative super-resolution microscopy. In this technique, we place a thin diffuser (i.e., a scattering lens) in between the sample and the…

Image and Video Processing · Electrical Eng. & Systems 2019-07-26 Pengming Song , Shaowei Jiang , He Zhang , Zichao Bian , Chengfei Guo , Kazunori Hoshino , Guoan Zheng

In this work, we propose a structured illumination (SI) method based on a two-photon excitation (TPE) scanning laser beam. Advantages of TPE methods include optical sectioning, low photo-toxicity, and robustness in the face of sample…

Biological Physics · Physics 2016-09-16 Alex Bardales , Qinggele Li , Bingzhao Zhu , Xiaodong Tao , Marc Reinig , Joel Kubby

Fluorescence polarization microscopy images both the intensity and orientation of fluorescent dipoles, which plays a vital role in studying the molecular structure and dynamics of bio-complex. However, it is difficult to resolve the dipole…