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Background: Spatial transcriptomics have emerged as a powerful tool in biomedical research because of its ability to capture both the spatial contexts and abundance of the complete RNA transcript profile in organs of interest. However,…

Genomics · Quantitative Biology 2025-04-18 Shuo Shuo Liu , Shikun Wang , Yuxuan Chen , Anil K. Rustgi , Ming Yuan , Jianhua Hu

Accurate detection of cancer tissue regions (CTR) enables deeper analysis of the tumor microenvironment and offers crucial insights into treatment response. Traditional CTR detection methods, which typically rely on the rich cellular…

Computer Vision and Pattern Recognition · Computer Science 2026-03-09 Shuailin Xue , Jun Wan , Lihua Zhang , Wenwen Min

Whole slide images (WSIs) enable weakly supervised prognostic modeling via multiple instance learning (MIL). Spatial transcriptomics (ST) preserves in situ gene expression, providing a spatial molecular context that complements morphology.…

Computer Vision and Pattern Recognition · Computer Science 2026-02-16 Lihe Liu , Xiaoxi Pan , Yinyin Yuan , Lulu Shang

Spatial transcriptomics (ST) enables transcriptome-wide profiling while preserving the spatial context of tissues, offering unprecedented opportunities to study tissue organization and cell-cell interactions in situ. Despite recent…

Computer Vision and Pattern Recognition · Computer Science 2026-01-21 Wei Wang , Quoc-Toan Ly , Chong Yu , Jun Bai

Histopathology remains the gold standard for cancer diagnosis and prognosis. With the advent of transcriptome profiling, multi-modal learning combining transcriptomics with histology offers more comprehensive information. However, existing…

Image and Video Processing · Electrical Eng. & Systems 2026-03-03 Yupei Zhang , Xiaofei Wang , Anran Liu , Lequan Yu , Chao Li

While pathology foundation models have transformed cancer image analysis, they often lack integration with molecular data at single-cell resolution, limiting their utility for precision oncology. Here, we present PAST, a pan-cancer…

Quantitative Methods · Quantitative Biology 2025-07-10 Changchun Yang , Haoyang Li , Yushuai Wu , Yilan Zhang , Yifeng Jiao , Yu Zhang , Rihan Huang , Yuan Cheng , Yuan Qi , Xin Guo , Xin Gao

Tumour heterogeneity in breast cancer poses challenges in predicting outcome and response to therapy. Spatial transcriptomics technologies may address these challenges, as they provide a wealth of information about gene expression at the…

Image and Video Processing · Electrical Eng. & Systems 2023-09-19 Md Mamunur Rahaman , Ewan K. A. Millar , Erik Meijering

Integrating whole-slide images (WSIs) and bulk transcriptomics for predicting patient survival can improve our understanding of patient prognosis. However, this multimodal task is particularly challenging due to the different nature of…

Computer Vision and Pattern Recognition · Computer Science 2024-04-16 Guillaume Jaume , Anurag Vaidya , Richard Chen , Drew Williamson , Paul Liang , Faisal Mahmood

Spatial Transcriptomics (ST) merges the benefits of pathology images and gene expression, linking molecular profiles with tissue structure to analyze spot-level function comprehensively. Predicting gene expression from histology images is a…

Computer Vision and Pattern Recognition · Computer Science 2025-12-01 Chen Zhang , Yilu An , Ying Chen , Hao Li , Xitong Ling , Lihao Liu , Junjun He , Yuxiang Lin , Zihui Wang , Rongshan Yu

Tissue phenotyping is a fundamental task in learning objective characterizations of histopathologic biomarkers within the tumor-immune microenvironment in cancer pathology. However, whole-slide imaging (WSI) is a complex computer vision in…

Computer Vision and Pattern Recognition · Computer Science 2022-03-02 Richard J. Chen , Rahul G. Krishnan

Spatial transcriptomics is a technology that captures gene expression levels at different spatial locations, widely used in tumor microenvironment analysis and molecular profiling of histopathology, providing valuable insights into…

Computer Vision and Pattern Recognition · Computer Science 2025-06-11 Junzhuo Liu , Markus Eckstein , Zhixiang Wang , Friedrich Feuerhake , Dorit Merhof

Advances in spatial transcriptomics (ST) technologies enable systematic molecular characterization of tumor microenvironment, tumor gradients and gene regulatory networks. Cancer progression is known to vary along pathological gradients,…

With the development of digital imaging in medical microscopy, artificial intelligent-based analysis of pathological whole slide images (WSIs) provides a powerful tool for cancer diagnosis. Limited by the expensive cost of pixel-level…

Computer Vision and Pattern Recognition · Computer Science 2024-11-19 Jiawen Li , Qiehe Sun , Renao Yan , Yizhi Wang , Yuqiu Fu , Yani Wei , Tian Guan , Huijuan Shi , Yonghonghe He , Anjia Han

The development of computational pathology lies in the consensus that pathological characteristics of tumors are significant guidance for cancer diagnostics. Most existing research focuses on the inner-contextual information within each WSI…

Computer Vision and Pattern Recognition · Computer Science 2024-10-15 Jun Shi , Tong Shu , Zhiguo Jiang , Wei Wang , Haibo Wu , Yushan Zheng

While spatial transcriptomics (ST) has advanced our understanding of gene expression in tissue context, its high experimental cost limits its large-scale application. Predicting ST from pathology images is a promising, cost-effective…

Computer Vision and Pattern Recognition · Computer Science 2026-03-25 Zhiceng Shi , Changmiao Wang , Jun Wan , Wenwen Min

Histomorphology is crucial in cancer diagnosis. However, existing whole slide image (WSI) classification methods struggle to effectively incorporate histomorphology information, limiting their ability to capture key pathological features.…

Computer Vision and Pattern Recognition · Computer Science 2025-12-01 Baizhi Wang , Rui Yan , Wenxin Ma , Xu Zhang , Yuhao Wang , Xiaolong Li , Yunjie Gu , Zihang Jiang , S. Kevin Zhou

Spatial transcriptomics (ST) enables the visualization of gene expression within the context of tissue morphology. This emerging discipline has the potential to serve as a foundation for developing tools to design precision medicines.…

Image and Video Processing · Electrical Eng. & Systems 2024-11-12 Shivam Kumar , Samrat Chatterjee

Learning good representation of giga-pixel level whole slide pathology images (WSI) for downstream tasks is critical. Previous studies employ multiple instance learning (MIL) to represent WSIs as bags of sampled patches because, for most…

Computer Vision and Pattern Recognition · Computer Science 2022-12-01 Chunyuan Li , Xinliang Zhu , Jiawen Yao , Junzhou Huang

Multimodal survival methods combining gigapixel histology whole-slide images (WSIs) and transcriptomic profiles are particularly promising for patient prognostication and stratification. Current approaches involve tokenizing the WSIs into…

Computer Vision and Pattern Recognition · Computer Science 2024-07-02 Andrew H. Song , Richard J. Chen , Guillaume Jaume , Anurag J. Vaidya , Alexander S. Baras , Faisal Mahmood

Spatial transcriptomics (ST) technologies have revolutionized the study of gene expression patterns in tissues by providing multimodality data in transcriptomic, spatial, and morphological, offering opportunities for understanding tissue…

Computational Engineering, Finance, and Science · Computer Science 2024-01-17 Zelin Zang , Liangyu Li , Yongjie Xu , Chenrui Duan , Kai Wang , Yang You , Yi Sun , Stan Z. Li
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