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The ribosome is a macromolecular complex which is responsible for protein synthesis in all living cells according to their transcribed genetic information. Using X-ray crystallography and, more recently, cryo-electron microscopy (cryo-EM),…

Biological Physics · Physics 2017-11-17 Lars V. Bock , Michal H. Kolář , Helmut Grubmüller

Translation of proteins is a fundamental part of gene expression that is mediated by ribosomes. As ribosomes significantly contribute to both cellular mass and energy consumption, achieving efficient management of the ribosome population is…

Subcellular Processes · Quantitative Biology 2024-04-18 Clément Soubrier , Eric Foxall , Luca Ciandrini , Khanh Dao Duc

Ribosome is a molecular machine that polymerizes a protein where the sequence of the amino acid residues, the monomers of the protein, is dictated by the sequence of codons (triplets of nucleotides) on a messenger RNA (mRNA) that serves as…

Biological Physics · Physics 2011-01-28 Ajeet K. Sharma , Debashish Chowdhury

The ribosome is one of the largest and most complex macromolecular machines in living cells. It polymerizes a protein in a step-by-step manner as directed by the corresponding nucleotide sequence on the template messenger RNA (mRNA) and…

Statistical Mechanics · Physics 2020-03-11 Annwesha Dutta , Gunter M Schütz , Debashish Chowdhury

Synthesis of protein molecules in a cell are carried out by ribosomes. A ribosome can be regarded as a molecular motor which utilizes the input chemical energy to move on a messenger RNA (mRNA) track that also serves as a template for the…

Biological Physics · Physics 2009-07-12 Ashok Garai , Debanjan Chowdhury , Debashish Chowdhury , T. V. Ramakrishnan

Translation is one of the most fundamental processes in the biological cell. Because of the central role that translation plays across all domains of life, the enzyme that carries out this process, the ribosome, is required to process…

Quantitative Methods · Quantitative Biology 2023-10-11 Daniel A. Inafuku , Kay L. Kirkpatrick , Onyema Osuagwu , Qier An , David A. Brewster , Mayisha Zeb Nakib

The process of polymerizing a protein by a ribosome, using a messenger RNA (mRNA) as the corresponding template, is called {\it translation}. Ribosome may be regarded as a molecular motor for which the mRNA template serves also as the…

Biological Physics · Physics 2015-03-19 Ajeet K. Sharma , Debashish Chowdhury

In permissive environments, E. coli can double its dry mass every 21 minutes. During this time, ribosomes, RNA polymerases, and the proteome are all doubled. Yet, the question of how to relate bacterial doubling time to other biologically…

Subcellular Processes · Quantitative Biology 2018-07-25 Rami Pugatch , Yinon M. Bar-on

Life demonstrates remarkable homochirality of its major building blocks: nucleic acids, amino acids, sugars, and phospholipids. We propose a mechanism that places the root of life homochirality in the formation of phospholipid bilayer…

Populations and Evolution · Quantitative Biology 2025-12-30 Vladimir Subbotin , Gennady Fiksel

Microbes require several complex organic molecules for growth. A species may obtain a required factor by taking up molecules released by other species or by synthesizing the molecule. The patterns of uptake and synthesis set a flow of…

Populations and Evolution · Quantitative Biology 2014-02-27 Steven A. Frank

Life occurs in concentrated `Ringer Solutions' derived from seawater that Lesser Blum studied for most of his life. As we worked together, Lesser and I realized that the questions asked of those solutions were quite different in biology…

Other Quantitative Biology · Quantitative Biology 2021-09-24 Bob Eisenberg

As I compress on the canvas of a few pages here major results of my research on the retinoblastoma tumor suppressor protein (RB) spreading over the past 15 years, an exciting picture emerges on this unique host molecule which surpasses in…

Biomolecules · Quantitative Biology 2007-06-15 Razvan Tudor Radulescu

Since the Hadean era of Earth's history, peptides/proteins and RNA have undergone a complex evolutionary trajectory. Originating from simple monomeric units, these molecules evolved abiotically under various biochemical and biophysical…

Biological Physics · Physics 2025-05-06 Michal H. Kolář , Klára Hlouchová

The thermosynthesis concept, biological free energy gain from thermal cycling, is combined with the concept of the RNA World. The resulting overall origin of life model gives new explanations for the emergence of the genetic code and the…

Populations and Evolution · Quantitative Biology 2007-05-23 Anthonie W. J. Muller

The number of ribosomes in a cell is considered as limiting, and gene expression is thus largely determined by their cellular concentration. In this work we develop a toy model to study the trade-off between the ribosomal supply and the…

Subcellular Processes · Quantitative Biology 2020-02-19 Pascal S. Rogalla , Timothy J. Rudge , Luca Ciandrini

The multifarious internal workings of organisms are difficult to reconcile with a single feature defining a state of being alive. Indeed, definitions of life rely on emergent properties (growth, capacity to evolve, agency) only symptomatic…

Other Quantitative Biology · Quantitative Biology 2023-08-16 Simon Pierce

Despite growth being fundamental to all aspects of cell biology, we do not yet know its organizing principles in eukaryotic cells. Classic models derived from the bacteria E. coli posit that protein-synthesis rates are set by mass-action…

Populations and Evolution · Quantitative Biology 2025-08-22 Xin Gao , Michael Lanz , Rosslyn Grosely , Jonas Cremer , Joseph Puglisi , Jan M. Skotheim

All proteins in living organisms are produced in ribosomes that facilitate the translation of genetic information into a sequence of amino acid residues. During translation, the ribosome undergoes initiation, elongation, termination, and…

Biological Physics · Physics 2024-09-20 Michal H. Kolář , Hugo Mc Grath , Felipe C. Nepomuceno , Michaela Černeková

Understanding the regulation and structure of ribosomes is essential to understanding protein synthesis and its deregulation in disease. While ribosomes are believed to have a fixed stoichiometry among their core ribosomal proteins (RPs),…

Genomics · Quantitative Biology 2015-11-24 Nikolai Slavov , Sefan Semrau , Edoardo Airoldi , Bogdan Budnik , Alexander van Oudenaarden

Protein molecules in cells are synthesized by macromolecular machines called ribosomes. According to recent experimental data, we reduce the complexity of the ribosome and propose a model to express its activity in six main states. Using…

Subcellular Processes · Quantitative Biology 2016-07-26 Bahareh Shakiba , Maryam Dayeri , Farshid Mohammad-Rafiee
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