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Electron microscopes have been improved to achieve ever smaller beam spots, a key parameter that determines the instrument's resolution. The techniques to measure the size of the beam, however, have not progressed to the same degree. There…

Applied Physics · Physics 2019-07-24 Matthew Zotta , Sharadh Jois , Prathamesh Dhakras , Miguel Rodriguez , Ji Ung Lee

Monitoring the activity of the heart is important for diagnosing and preventing cardiovascular diseases. The electrocardiogram (ECG) is the gold standard for diagnosing such diseases. It monitors the heart's electrical activity, and while…

Signal Processing · Electrical Eng. & Systems 2023-09-19 Rémi Grisot , Pierre Laurent , Claire Migliaccio , Jean-Yves Dauvignac , Mélanie Brulc , Camille Chiquet , Jean-Paul Caruana

The aim of this paper is to give an introductory review of the cryoelectron microscopy as a complex data source for the most of the system biology branches, including the most perspective non-local approaches known as "localomics" and…

Subcellular Processes · Quantitative Biology 2015-01-20 Oleg V. Gradov , Margaret A. Gradova

The Electrocardiograph signal represents the heart's electrical activity while blood pressure results from the heart's mechanical activity. Previous studies have investigated how the heart's electrical and mechanical activities are related…

Signal Processing · Electrical Eng. & Systems 2020-08-25 Seyedeh Somayyeh Mousavi , Mostafa Charmi , Mohammad Firouzmand , Mohammad Hemmati , Maryam Moghadam , Yadollah Ghorbani

Aberration-corrected scanning transmission electron microscopes (STEM) provide sub-angstrom lateral resolution; however, the large convergence angle greatly reduces the depth of field. For microscopes with a small depth of field,…

Materials Science · Physics 2011-12-15 Robert Hovden , Huolin L. Xin , David A. Muller

Electron and x-ray microscopes allow one to image the entire, unlabeled structure of hydrated materials at a resolution well beyond what visible light microscopes can achieve. However, both approaches involve ionizing radiation, so that…

Applied Physics · Physics 2020-04-22 Ming Du , Chris Jacobsen

The physical and chemical environment inside cells is of fundamental importance to all life but has traditionally been difficult to determine on a subcellular basis. Here we combine cutting-edge genomically integrated FRET biosensing to…

Cell Behavior · Quantitative Biology 2020-10-22 Jack W Shepherd , Sarah Lecinski , Jasmine Wragg , Sviatlana Shashkova , Chris MacDonald , Mark C Leake

In photoelectron circular dichroism (PECD) it is generally difficult to trace how and when the chirality of the molecule is imprinted onto the photoelectron. We present simulations of PECD in a simple model and employ chirality measures to…

Atomic Physics · Physics 2025-03-04 Marec W. Heger , Daniel M. Reich

We demonstrate the merits of using a neuromorphic, or event-based camera (EBC), for tracking of both passive and active matter. For passive matter, we tracked the Brownian motion of different micro-particles and estimated their diffusion…

Electron energy loss spectroscopy is consolidating as a powerful tool to explore electronic (as well as vibrational) excitations of matter, including molecules. Performed in a scanning transmission electron microscope, this technique is…

Chemical Physics · Physics 2021-03-05 Ciro A. Guido , Enzo Rotunno , Matteo Zanfrognini , Stefano Corni , Vincenzo Grillo

Electrochemiluminescence (ECL) produces light through electrochemical reactions and has shown promise for various analytic applications in biomedicine. However, the use of ECL devices (ECLDs) as light sources has been limited due to…

Single molecules that exhibit narrow optical transitions at cryogenic temperatures can be used as local electric-field sensors. We derive the single charge sensitivity of aromatic organic dye molecules, based on first principles. Through…

Mesoscale and Nanoscale Physics · Physics 2015-06-23 Sanli Faez , Sense Jan van der Molen , Michel Orrit

Bacteria sense a diverse range of environmental analytes with high sensitivity and temporal resolution. Engineering and synthetic biology approaches enabled harnessing this capability through development of whole-cell biosensors that…

Simultaneous electrocardiography (ECG) and phonocardiogram (PCG) provide a comprehensive, multimodal perspective on cardiac function by capturing the heart's electrical and mechanical activities, respectively. However, the distinct and…

Machine Learning · Computer Science 2025-06-13 Sajjad Karimi , Amit J. Shah , Gari D. Clifford , Reza Sameni

Low-light optical imaging refers to the use of cameras to capture images with minimal photon flux. This area has broad application to diverse fields, including optical microscopy for biological studies. In such studies, it is important to…

We describe the characteristics of low-cost ultra-high-power light emitting diodes (LEDs) for use in optical imaging experiments. We use the LEDs in experiments with bullfrog cardiac tissue and find that the signal-to-noise ratio is…

Biological Physics · Physics 2012-08-27 H. M. Dobrovolny , H. Elmariah , S. S. Kalb , J. P. Wikswo, , D. J. Gauthier

Over the last decade, single-molecule optical microscopy has become the gold-standard approach to decipher complex molecular processes in cellular environments. [1-3] Single-molecule fluorescence microscopy has several advantages such as…

Biological Physics · Physics 2017-11-15 Edakkattuparambil Sidharth Shibu , Nadezda Varkentina , Laurent Cognet , Brahim Lounis

Space-based stellar coronagraph instruments aim to directly image exoplanets that are a fraction of an arcsecond separation and ten billion times fainter than their host star. To achieve this, one or more deformable mirrors (DMs) are used…

Echocardiography (echo), or cardiac ultrasound, is the most widely used imaging modality for cardiac form and function due to its relatively low cost, rapid acquisition time, and non-invasive nature. However, ultrasound acquisitions are…

Quantitative Methods · Quantitative Biology 2025-07-18 David Choi , Milos Vukadinovic , Bryan He , Christina Binder , Yuki Sahashi , David Ouyang

In the past decade, deep conditional generative models have revolutionized the generation of realistic images, extending their application from entertainment to scientific domains. Single-particle cryo-electron microscopy (cryo-EM) is…

Computer Vision and Pattern Recognition · Computer Science 2024-10-01 Jiakai Zhang , Qihe Chen , Yan Zeng , Wenyuan Gao , Xuming He , Zhijie Liu , Jingyi Yu