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Spatial Transcriptomics is a novel technology that aligns histology images with spatially resolved gene expression profiles. Although groundbreaking, it struggles with gene capture yielding high corruption in acquired data. Given potential…

Computer Vision and Pattern Recognition · Computer Science 2024-09-30 Gabriel Mejia , Daniela Ruiz , Paula Cárdenas , Leonardo Manrique , Daniela Vega , Pablo Arbeláez

Medical images acquired from standardized protocols show consistent macroscopic or microscopic anatomical structures, and these structures consist of composable/decomposable organs and tissues, but existing self-supervised learning (SSL)…

Computer Vision and Pattern Recognition · Computer Science 2025-01-20 Ziyu Zhou , Haozhe Luo , Mohammad Reza Hosseinzadeh Taher , Jiaxuan Pang , Xiaowei Ding , Michael Gotway , Jianming Liang

Multiplex immunofluorescence (mIF) imaging technology facilitates the study of the tumour microenvironment in cancer patients. Due to the capabilities of this emerging bioimaging technique, it is possible to statistically analyse, for…

Applications · Statistics 2023-07-07 Jonatan A. González , Julia Wrobel , Simon Vandekar , Paula Moraga

Understanding the intricate cellular environment within biological tissues is crucial for uncovering insights into complex biological functions. While single-cell RNA sequencing has significantly enhanced our understanding of cellular…

Image and Video Processing · Electrical Eng. & Systems 2024-11-06 Bingjun Li , Mostafa Karami , Masum Shah Junayed , Sheida Nabavi

This study evaluates the generalisation capabilities of state-of-the-art histopathology foundation models on out-of-distribution multi-stain autoimmune Immunohistochemistry datasets. We compare 13 feature extractor models, including…

Computer Vision and Pattern Recognition · Computer Science 2024-10-30 Amaya Gallagher-Syed , Elena Pontarini , Myles J. Lewis , Michael R. Barnes , Gregory Slabaugh

Feature engineering continues to play a critical role in image classification, particularly when interpretability and computational efficiency are prioritized over deep learning models with millions of parameters. In this study, we revisit…

Computer Vision and Pattern Recognition · Computer Science 2026-02-06 Abhijit Sen , Giridas Maiti , Bikram K. Parida , Bhanu P. Mishra , Mahima Arya , Denys I. Bondar

Sparse autoencoders (SAEs) emerged as a promising tool for mechanistic interpretability of transformer-based foundation models. Very recently, SAEs were also adopted for the visual domain, enabling the discovery of visual concepts and their…

Computer Vision and Pattern Recognition · Computer Science 2025-07-17 Muhammed Furkan Dasdelen , Hyesu Lim , Michele Buck , Katharina S. Götze , Carsten Marr , Steffen Schneider

In recent years, histopathology images have been increasingly used as a diagnostic tool in the medical field. The process of accurately diagnosing a biopsy sample requires significant expertise in the field, and as such can be…

Image and Video Processing · Electrical Eng. & Systems 2019-09-24 Alison K. Cheeseman , Hamid Tizhoosh , Edward R. Vrscay

One of the major challenges in biology concerns the integration of data across length and time scales into a consistent framework: how do macroscopic properties and functionalities arise from the molecular regulatory networks - and how can…

Computational Engineering, Finance, and Science · Computer Science 2015-10-20 Dagmar Iber , Zahra Karimaddini , Erkan Ünal

With the remarkable success of representation learning for prediction problems, we have witnessed a rapid expansion of the use of machine learning and deep learning for the analysis of digital pathology and biopsy image patches. However,…

Machine Learning · Computer Science 2024-03-06 David Ahmedt-Aristizabal , Mohammad Ali Armin , Simon Denman , Clinton Fookes , Lars Petersson

Hematoxylin- and eosin (H&E) stained whole-slide images (WSIs) are the foundation of diagnosis of cancer. In recent years, development of deep learning-based methods in computational pathology enabled the prediction of biomarkers directly…

Spatial transcriptomics (ST) provides essential spatial context by mapping gene expression within tissue, enabling detailed study of cellular heterogeneity and tissue organization. However, aligning ST data with histology images poses…

Spatial transcriptomics enables interrogating the molecular composition of tissue with ever-increasing resolution and sensitivity. However, costs, rapidly evolving technology, and lack of standards have constrained computational methods in…

Pathology foundation models learn morphological representations through self-supervised pretraining on large-scale whole-slide images, yet they do not explicitly capture the underlying molecular state of the tissue. Spatial transcriptomics…

Computer Vision and Pattern Recognition · Computer Science 2026-03-10 Minsoo Lee , Jonghyun Kim , Juseung Yun , Sunwoo Yu , Jongseong Jang

The emergence of foundation models in computational pathology has transformed histopathological image analysis, with whole slide imaging (WSI) diagnosis being a core application. Traditionally, weakly supervised fine-tuning via multiple…

Computer Vision and Pattern Recognition · Computer Science 2025-03-03 Jiawen Li , Jiali Hu , Qiehe Sun , Renao Yan , Minxi Ouyang , Tian Guan , Anjia Han , Chao He , Yonghong He

Hierarchical multiplex imaging approaches generate spatially resolved single-cell measurements across multiple, spatially organized fields of view (FOVs) within patient tumor specimens, thereby enabling systematic investigation of how the…

Topological correctness is critical for segmentation of tubular structures, which pervade in biomedical images. Existing topological segmentation loss functions are primarily based on the persistent homology of the image. They match the…

Computer Vision and Pattern Recognition · Computer Science 2025-10-29 Bo Wen , Haochen Zhang , Dirk-Uwe G. Bartsch , William R. Freeman , Truong Q. Nguyen , Cheolhong An

Histopathology refers to the examination by a pathologist of biopsy samples. Histopathology images are captured by a microscope to locate, examine, and classify many diseases, such as different cancer types. They provide a detailed view of…

Image and Video Processing · Electrical Eng. & Systems 2020-11-12 Naira Elazab , Hassan Soliman , Shaker El-Sappagh , S. M. Riazul Islam , Mohammed Elmogy

Accurate diagnosis and treatment of complex diseases require integrating histological, molecular, and clinical data, yet in practice these modalities are often incomplete owing to tissue scarcity, assay cost, and workflow constraints.…

Computer Vision and Pattern Recognition · Computer Science 2026-04-07 Jinxi Xiang , Mingjie Li , Siyu Hou , Yijiang Chen , Xiangde Luo , Yuanfeng Ji , Xiang Zhou , Ehsan Adeli , Akshay Chaudhari , Curtis P. Langlotz , Kilian M. Pohl , Ruijiang Li

The growing volume of high-resolution Whole Slide Images in digital histopathology poses significant storage, transmission, and computational efficiency challenges. Standard compression methods, such as JPEG, reduce file sizes but often…

Image and Video Processing · Electrical Eng. & Systems 2025-03-17 Srikar Yellapragada , Alexandros Graikos , Kostas Triaridis , Zilinghan Li , Tarak Nath Nandi , Ravi K Madduri , Prateek Prasanna , Joel Saltz , Dimitris Samaras
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