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A ubiquitous feature of living cells is their growth over time followed by division into daughter cells. How isogenic cell populations maintain size homeostasis, i.e., a narrow distribution of cell size, is an intriguing fundamental…

Cell Behavior · Quantitative Biology 2016-06-03 César Augusto Vargas-García , Mohammad Soltani , Abhyudai Singh

Single-cell experiments revealed substantial variability in generation times, growth rates but also in birth and division sizes between genetically identical cells. Understanding how these fluctuations determine the fitness of the…

Populations and Evolution · Quantitative Biology 2025-03-24 Arthur Genthon

Our understanding of cell division control in bacteria still relies largely on interpreting correlations between phenomenological variables, with limited connection to the underlying molecular mechanisms. Here, we analytically solve a…

Cells employ control strategies to maintain a stable size. Dividing at a target size (the `sizer' strategy) is thought to produce the tightest size distribution. However, this result follows from phenomenological models that ignore the…

Biological Physics · Physics 2024-02-27 Motasem ElGamel , Andrew Mugler

Organisms across all domains of life regulate the size of their cells. However, the means by which this is done is poorly understood. We study two abstracted "molecular" models for size regulation: inhibitor dilution and initiator…

Molecular Networks · Quantitative Biology 2017-11-07 Felix Barber , Po-Yi Ho , Andrew W. Murray , Ariel Amir

The mechanism of bacterial cell size control has been a mystery for decades, which involves the well-coordinated growth and division in the cell cycle. The revolutionary modern techniques of microfluidics and the advanced live imaging…

Statistical Mechanics · Physics 2021-08-30 Liang Luo , Yang Bai , Xiongfei Fu

Cells control their size to cope with noise during growth and division. Eukaryotic cells exhibiting "sizer" control (targeting a specific size before dividing) may rely on molecular concentration thresholds, but simple implementations of…

Biological Physics · Physics 2026-05-08 Motasem ElGamel , Lucas Ribaudo , Andrew Mugler

Cell size control is crucial for maintaining cellular function and homeostasis. In this study, we develop a first-order partial differential equation model to examine the effects of three key size control mechanisms: the sizer, timer, and…

Cell Behavior · Quantitative Biology 2025-05-27 Shuqi Fan , Jinzhi Lei

How exponentially growing cells maintain size homeostasis is an important fundamental problem. Recent single-cell studies in prokaryotes have uncovered the adder principle, where cells on average, add a fixed size (volume) from birth to…

Subcellular Processes · Quantitative Biology 2016-07-28 Khem Raj Ghusinga , Cesar A. Vargas-Garcia , Abhyudai Singh

Cells maintain a stable size as they grow and divide. Inspired by the available experimental data, most proposed models for size homeostasis assume size control mechanisms that act on a timescale of one generation. Such mechanisms lead to…

Biological Physics · Physics 2023-05-25 Motasem ElGamel , Harsh Vashistha , Hanna Salman , Andrew Mugler

Cell growth in size is a complex process coordinated by intrinsic and environmental signals. In a recent work [Tzur et al., Science, 2009, 325:167-171], size distributions in an exponentially growing population of mammalian cells were used…

Cell Behavior · Quantitative Biology 2015-06-16 Yucheng Hu , Tianqi Zhu

Fundamental mechanisms governing cell size control and homeostasis are still poorly understood. The relationship between sizes at division and birth in single cells is used as a metric to categorize the basis of size homeostasis [1-3].…

Cell Behavior · Quantitative Biology 2020-01-08 Giuseppe Facchetti , Benjamin Knapp , Fred Chang , Martin Howard

Microbial growth and division are fundamental processes relevant to many areas of life science. Of particular interest are homeostasis mechanisms, which buffer growth and division from accumulating fluctuations over multiple cycles. These…

Cell Behavior · Quantitative Biology 2022-10-12 Lee Susman , Maryam Kohram , Harsh Vashistha , Jeffrey T. Nechleba , Hanna Salman , Naama Brenner

We study the effect of correlations in generation times on the dynamics of population growth of microorganisms. We show that any non-zero correlation that is due to cell-size regulation, no matter how small, induces long-term oscillations…

Populations and Evolution · Quantitative Biology 2019-03-27 Farshid Jafarpour

Various rod-shaped bacteria such as the canonical gram negative Escherichia coli or the well-studied gram positive Bacillus subtilis divide symmetrically after they approximately double their volume. Their size at division is not constant,…

Cell Behavior · Quantitative Biology 2015-06-18 Ariel Amir

The rate at which individual bacterial cells grow depends on the concentrations of cellular components such as ribosomes and proteins. These concentrations continuously fluctuate over time and are inherited from mother to daughter cells,…

Populations and Evolution · Quantitative Biology 2024-08-21 Yaïr Hein , Farshid Jafarpour

Classic models of cell size control consider cells divide while reaching a threshold, e.g. size, age, or size extension. The molecular basis of the threshold involves multiple layers of regulation as well as gene noises. In this work, we…

Statistical Mechanics · Physics 2022-04-28 Liang Luo , Yang Bai , Xiongfei Fu

Recent experiments have supported the Adder model for E. coli division control. This model posits that bacteria grow, on average, a fixed size before division. It also predicts decorrelation between the noise in the added size and the size…

Solving population balance equations, we derive analytical steady-state cell size distributions for single-lineage experiments, such as the mother machine. These experiments are fundamentally different from batch cultures where populations…

Populations and Evolution · Quantitative Biology 2023-09-13 Arthur Genthon

Establishing a quantitative connection between the population growth rate and the generation times of single cells is a prerequisite for understanding evolutionary dynamics of microbes. However, existing theories fail to account for the…

Populations and Evolution · Quantitative Biology 2017-09-19 Jie Lin , Ariel Amir
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