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ATP synthases utilize a proton motive force to synthesize ATP. In reverse, these membrane-embedded enzymes can also hydrolyze ATP to pump protons over the membrane. To prevent wasteful ATP hydrolysis, distinct control mechanisms exist for…

Biomolecules · Quantitative Biology 2018-01-16 Hendrik Sielaff , Dhirendra Singh , Gerhard Grueber , Michael Börsch

Microbial electrolysis cells (MECs) employ electroactive bacteria to perform extracellular electron transfer, enabling hydrogen generation from biodegradable substrates. In previous work, we developed and analyzed a differential-algebraic…

Populations and Evolution · Quantitative Biology 2022-06-03 Harry J. Dudley , Zhiyong Jason Ren , David M. Bortz

Bacteria such as Escherichia coli (E. coli) exhibit biased motion if kept in a spatially non-uniform chemical environment. Here, we bring out unique time-dependent characteristics of bacterial chemotaxis, in response to a diffusing spatial…

Biological Physics · Physics 2018-12-05 Sibendu Samanta , Ritwik Layek , Shantimoy Kar , Sudipta Mukhopadhyay , Suman Chakraborty

Conjugation is the primary mechanism of horizontal gene transfer that spreads antibiotic resistance among bacteria. Although conjugation normally occurs in surface-associated growth (e.g., biofilms), it has been traditionally studied in…

Quantitative Methods · Quantitative Biology 2014-02-26 Peter D. Freese , Kirill S. Korolev , Jose I. Jimenez , Irene A. Chen

DFT calculations performed on Si_2H_6, Si_2F_6, Si_2Cl_6, and Si_2Br_6 are reported. The evolution of the energy, the chemical potential and the molecular hardness, as a function of torsion angle, is studied. Results at the…

Materials Science · Physics 2009-11-07 Felipe Valencia , Aldo H. Romero , Miguel Kiwi , Ricardo Ramirez , Alejandro Toro-Labbe

The near-surface swimming patterns of bacteria are strongly determined by the hydrodynamic interactions between bacteria and the surface, which trap bacteria in smooth circular trajectories that lead to inefficient surface exploration.…

Background: The Epithelial-Mesenchymal Transition (EMT) endows epithelial-looking cells with enhanced migratory ability during embryonic development and tissue repair. EMT can also be co-opted by cancer cells to acquire metastatic potential…

Despite recent advances in our ability to detect adaptive evolution involving the cis-regulation of gene expression, our knowledge of the molecular mechanisms underlying these adaptations has lagged far behind. Across all model organisms…

Populations and Evolution · Quantitative Biology 2013-08-08 Jessica Chang , Yiqi Zhou , Xiaoli Hu , Lucia Lam , Cameron Henry , Erin M. Green , Ryosuke Kita , Michael S. Kobor , Hunter B. Fraser

The transcriptional regulation of gene expression is orchestrated by complex networks of interacting genes. Increasing evidence indicates that these transcriptional regulatory networks (TRNs) in bacteria have an inherently hierarchical…

Molecular Networks · Quantitative Biology 2014-05-16 Santhust Kumar , Michele Vendruscolo , Amit Singh , Dhiraj Kumar , Areejit Samal

In a letter published in Molecular Biology Evolution [10], Chen and Zhang argue that the variation of the mutation rate along the Escherichia coli genome that we recently reported [3] cannot be evolutionarily optimised. To support this…

Genomics · Quantitative Biology 2013-05-09 Inigo Martincorena , Nicholas M. Luscombe

Intracellular transport is an essential function in eucaryotic cells, facilitated by motor proteins - proteins converting chemical energy into kinetic energy. It is known that motor proteins work in teams enabling unidirectional and…

Subcellular Processes · Quantitative Biology 2016-03-29 Shreyas Bhaban , Donatello Materassi , Mingang Li , Thomas Hays , Murti Salapaka

A recently experimentally observed biochemical "threshold filtering" mechanism by processes catalyzed by the enzyme malate dehydrogenase is explained in terms of a model that incorporates an unusual mechanism of inhibition of this enzyme…

Molecular Networks · Quantitative Biology 2014-11-11 Vladimir Privman , Sergii Domanskyi , Shay Mailloux , Yaovi Holade , Evgeny Katz

We study model one-dimensional chemical systems (representative of their three-dimensional counterparts) using the strictly-correlated electrons (SCE) functional, which, by construction, becomes asymptotically exact in the limit of infinite…

Elucidating the architecture and dynamics of large scale genetic regulatory networks of cells is an important goal in systems biology. We study the system level dynamical properties of the genetic network of Escherichia coli that regulates…

Molecular Networks · Quantitative Biology 2008-02-29 Areejit Samal , Sanjay Jain

S-adenosylhomocysteine hydrolase (SAHH), a cellular enzyme that plays a key role in methylation reactions including those required for maturation of viral mRNA, is an important drug target in the discovery of antiviral agents. While…

Biomolecules · Quantitative Biology 2017-01-24 Yoonji Lee , Lak Shin Jeong , Sun Choi , Changbong Hyeon

Understanding the organization of reaction fluxes in cellular metabolism from the stoichiometry and the topology of the underlying biochemical network is a central issue in systems biology. In this task, it is important to devise reasonable…

Molecular Networks · Quantitative Biology 2009-11-13 C. Martelli , A. De Martino , E. Marinari , M. Marsili , I. Perez Castillo

The active sites of enzymes consist of residues necessary for catalysis, and structurally important non-catalytic residues that together maintain the architecture and function of the active site. Examples of evolutionary interactions…

Biomolecules · Quantitative Biology 2014-06-06 Thomas A. McMurrough , Russel J. Dickson , Stephanie M. F. Thibert , Gregory B. Gloor , David R. Edgell

Purpose of review: The epithelial-Mesenchymal Transition (EMT) and the generation of Cancer Stem Cells (CSC) are two fundamental aspects contributing to tumor growth, acquisition of resistance to therapy, formation of metastases, and tumor…

Molecular Networks · Quantitative Biology 2018-11-07 Federico Bocci , Herbert Levine , José Nelson Onuchic , Mohit Kumar Jolly

Antimicrobial resistance (AMR) poses a significant threat to public health by increasing mortality, extending hospital stays, and increasing healthcare costs. It affects people of all ages and affects health services, veterinary medicine,…

Dynamical Systems · Mathematics 2023-06-12 Alissen Peterson , Jhoana P. Romero-Leiton , Pablo Aguirre , Kamal R. Acharya , Bouchra Nasri

Many proteins undergo conformational changes during their activity. A full understanding of the function of these proteins can only be obtained if different conformations and transitions between them can be monitored in aqueous solution,…