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We introduce a statistical procedure that integrates survival data from multiple biomedical studies, to improve the accuracy of predictions of survival or other events, based on individual clinical and genomic profiles, compared to models…

Applications · Statistics 2020-07-20 Steffen Ventz , Rahul Mazumder , Lorenzo Trippa

Single-cell perturbation studies face dual heterogeneity bottlenecks: (i) semantic heterogeneity--identical biological concepts encoded under incompatible metadata schemas across datasets; and (ii) statistical heterogeneity--distribution…

Artificial Intelligence · Computer Science 2026-03-10 Wenxuan Huang , Mingyu Tsoi , Yanhao Huang , Xinjie Mao , Xue Xia , Hao Wu , Jiaqi Wei , Yuejin Yang , Lang Yu , Cheng Tan , Xiang Zhang , Zhangyang Gao , Siqi Sun

Joint analysis of multi-omic single-cell data across cohorts has significantly enhanced the comprehensive analysis of cellular processes. However, most of the existing approaches for this purpose require access to samples with complete…

Machine Learning · Computer Science 2024-05-21 Marianne Arriola , Weishen Pan , Manqi Zhou , Qiannan Zhang , Chang Su , Fei Wang

Uniform Manifold Approximation and Projection (UMAP) is a widely used manifold learning technique for dimensionality reduction. This paper studies UMAP, supervised UMAP, and several competing dimensionality reduction methods, including…

Machine Learning · Computer Science 2026-05-04 Guanzhe Zhang , Shanshan Ding , Zhezhen Jin

In recent years, the advances in single-cell RNA-seq techniques have enabled us to perform large-scale transcriptomic profiling at single-cell resolution in a high-throughput manner. Unsupervised learning such as data clustering has become…

Genomics · Quantitative Biology 2020-01-07 Shixiong Zhang , Xiangtao Li , Qiuzhen Lin , Ka-Chun Wong

The advent of high dimensional single cell data in the biomedical sciences has necessitated the development of dimensionality-reduction tools. t-SNE and UMAP are the two most frequently used approaches, allowing clear visualisation of…

Quantitative Methods · Quantitative Biology 2021-12-09 Carlos P. Roca1 , Oliver T. Burton , Julika Neumann , Samar Tareen , Carly E. Whyte , Stéphanie Humblet-Baron , Adrian Liston

Single-cell RNA sequencing (scRNA-seq) has revolutionized our ability to analyze gene expression at the cellular level. By providing data on gene expression for each individual cell, scRNA-seq generates large datasets with thousands of…

Computational Complexity · Computer Science 2025-02-11 Md Romizul Islam , Swakkhar Shatabda

Single-cell technologies are revolutionizing the entire field of biology. The large volumes of data generated by single-cell technologies are high-dimensional, sparse, heterogeneous, and have complicated dependency structures, making…

Multiplexed imaging data are revolutionizing our understanding of the composition and organization of tissues and tumors. A critical aspect of such tissue profiling is quantifying the spatial relationship relationships among cells at…

Quantitative Methods · Quantitative Biology 2024-05-06 Ajit J. Nirmal , Peter K. Sorger

Single-cell RNA sequencing (scRNA-seq) is widely used to reveal heterogeneity in cells, which has given us insights into cell-cell communication, cell differentiation, and differential gene expression. However, analyzing scRNA-seq data is a…

Machine Learning · Computer Science 2023-06-27 Yuta Hozumi , Gu-Wei Wei

New technologies have enabled the investigation of biology and human health at an unprecedented scale and in multiple dimensions. These dimensions include a myriad of properties describing genome, epigenome, transcriptome, microbiome,…

Quantitative Methods · Quantitative Biology 2018-10-22 Marinka Zitnik , Francis Nguyen , Bo Wang , Jure Leskovec , Anna Goldenberg , Michael M. Hoffman

Single-cell RNA sequencing (scRNA-seq) provides unprecedented insights into cellular heterogeneity, enabling detailed analysis of complex biological systems at single-cell resolution. However, the high dimensionality and technical noise…

Genomics · Quantitative Biology 2025-09-04 Hojjat Torabi Goudarzi , Maziyar Baran Pouyan

Recent advances in single-cell technologies have advanced our understanding of gene regulation and cellular heterogeneity at single-cell resolution. Single-cell data contain both gene expression levels and the proportion of expressing…

Methodology · Statistics 2026-04-10 Seungjun Ahn , Donald Porchia , Panos Roussos , Maaike van Gerwen , Qing Lu , Zhigang Li

Modern single-cell flow and mass cytometry technologies measure the expression of several proteins of the individual cells within a blood or tissue sample. Each profiled biological sample is thus represented by a set of hundreds of…

Machine Learning · Computer Science 2022-06-29 Siyuan Shan , Vishal Baskaran , Haidong Yi , Jolene Ranek , Natalie Stanley , Junier Oliva

Cell heterogeneity and the inherent complexity due to the interplay of multiple molecular processes within the cell pose difficult challenges for current single-cell biology. We introduce an approach that identifies a disease phenotype from…

Background: Single-cell RNA sequencing (scRNA-seq) enables gene expression profiling at cellular resolution but is inherently affected by sparsity caused by dropout events, where expressed genes are recorded as zeros due to technical…

Genomics · Quantitative Biology 2026-04-15 Yuichiro Iwashita , Ahtisham Fazeel Abbasi , Koichi Kise , Andreas Dengel , Muhammad Nabeel Asim

Deep sequencing has become one of the most popular tools for transcriptome profiling in biomedical studies. While an abundance of computational methods exists for "normalizing" sequencing data to remove unwanted between-sample variations…

Genomics · Quantitative Biology 2022-01-14 Yannick Düren , Johannes Lederer , Li-Xuan Qin

In clinical practice, medical segmentation datasets are often limited and heterogeneous, with variations in modalities, protocols, and anatomical targets across institutions. Existing deep learning models struggle to jointly learn from such…

Computer Vision and Pattern Recognition · Computer Science 2026-01-22 Weiwei Ma , Xiaobing Yu , Peijie Qiu , Jin Yang , Pan Xiao , Xiaoqi Zhao , Xiaofeng Liu , Tomo Miyazaki , Shinichiro Omachi , Yongsong Huang

Cell populations are never truly homogeneous; individual cells exist in biochemical states that define functional differences between them. New technology based on microfluidic arrays combined with multiplexed quantitative polymerase chain…

Single-cell RNA-seq data allow the quantification of cell type differences across a growing set of biological contexts. However, pinpointing a small subset of genomic features explaining this variability can be ill-defined and…

Machine Learning · Statistics 2022-07-29 Nabeel Sarwar , Wilson Gregory , George A Kevrekidis , Soledad Villar , Bianca Dumitrascu