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There is a growing need for biolabels that can be used in both optical and electron microscopies, are non-cytotoxic, and do not photobleach. Such biolabels could enable targeted nanoscale imaging of sub-cellular structures, and help to…

Biological Physics · Physics 2015-06-23 Mark A. Zurbuchen , Michael P. Lake , Sirus A. Kohan , Belinda Leung , Louis-S. Bouchard

Fluorescent nanodiamonds (FNDs) hosting nitrogen-vacancy (NV) centers have attracted considerable attention for quantum sensing applications, particularly owing to notable advancements achieved in the field of weak magnetic signal detection…

Tracking the dynamics of fluorescent nanoparticles during embryonic development allows insights into the physical state of the embryo and, potentially, molecular processes governing developmental mechanisms. In this work, we investigate the…

Real-time temperature monitoring inside living organisms provides a direct measure of their biological activities, such as homeostatic thermoregulation and energy metabolism. However, it is challenging to reduce the size of bio-compatible…

Enzymes are cellular protein machines using a variety of conformational changes to power fast biochemical catalysis. Our goal is to exploit the single-spin properties of the luminescent NV (nitrogen-vacancy) center in nanodiamonds to reveal…

Quantitative Methods · Quantitative Biology 2024-01-25 Ivan Perez , Anke Krueger , Joerg Wrachtrup , Fedor Jelezko , Michael Börsch

Nanodiamond hosting temperature-sensing centers constitutes a closed thermodynamic system, with the only window of energy exchange with the environment without direct contacts of a sensor with intracellular substrates, which is in fact the…

The prospect of RNA nanotechnology is increasing because of its numerous potential applications especially in medical science. The spherical Nanodiamonds (NDs) are becoming popular because of their lesser toxicity, desirable mechanical,…

Optical bio-markers have been used extensively for intracellular imaging with high spatial and temporal resolution. Extending the modality of these probes is a key driver in cell biology. In recent years, the NV centre in nanodiamond has…

Recently, the implementation of plasmonic nanoantennas has opened new possibilities to investigate the nanoscale dynamics of individual biomolecules in living cell. However, studies have yet been restricted to single molecular species as…

Currently, the primary applications of fluorescent nanodiamonds (FNDs) are in the area of biosensing, by using photoluminescence or spin properties of colour centres, mainly represented by the Nitrogen Vacancy (NV) point defect. The…

Mesoscale and Nanoscale Physics · Physics 2026-05-19 Jeroen Prooth , Michael Petrov , Alevtina Shmakova , Michal Gulka , Petr Cigler , Jan D'Haen , Hans-Gerd Boyen , Milos Nesladek

The nitrogen-vacancy (NV) center in diamond exists in different charge states with distinct photoluminescence properties, which are sensitive to the nanoscale electrochemical environment. Hence, the NV charge state is emerging as a powerful…

Mesoscale and Nanoscale Physics · Physics 2026-05-12 Patrick Voorhoeve , Hiroshi Abe , Takeshi Ohshima , Qiang Sun , Anita Quigley , Rob Kapsa , Nikolai Dontschuk , Philipp Reineck

Traditional cellular force-sensing techniques, such as traction force microscopy (TFM), are predominantly limited to measuring linear tractions, overlooking and technically unable to capture the nanoscale torsional forces that are critical…

The tumor microenvironment (TME) features distinct hallmarks, including acidosis, hypoxia, reactive oxygen species (ROS) generation, and altered ion fluxes, which are crucial targets for early cancer biomarker detection, tumor diagnosis,…

A simple and convenient design enables real-time three-dimensional position tracking of nitrogen-vacancy (NV) centers in diamond. The system consists entirely of commercially available components (a single photon counter, a high-speed…

Instrumentation and Detectors · Physics 2018-03-14 Kiho Kim , Jiwon Yun , Donghyuck Lee , Dohun Kim

Nano-indentation based on, e.g., atomic force microscopy (AFM), can measure single cell elasticity with high spatial resolution and sensitivity, but relating the data to cell mechanical properties depends on modeling that requires knowledge…

Biological Physics · Physics 2021-09-29 Yue Cui , Weng-Hang Leong , Chu-Feng Liu , Kangwei Xia , Xi Feng , Csilla Gergely , Ren-Bao Liu , Quan Li

In recent years, plasmonic nanocavities have emerged as powerful tools for controlling and enhancing light-matter interactions at the nanoscale. This study explores the role of plasmonic nanocavities in manipulating nonadiabatic dynamics,…

Optics · Physics 2025-01-23 Yu Wang , Ruihao Bi , Wenjie Dou

Nitrogen-vacancy (NV) based quantum sensors hold great potential for real-time single-cell sensing with far-reaching applications in fundamental biology and medical diagnostics. Although highly sensitive, the mapping of quantum measurements…

Nitrogen-vacancy (NV) centers show great potentials for nanoscale bio-sensing and bio-imaging. Nevertheless, their envisioned bio-applications suffer from intrinsic background noise due to unavoidable light scattering and autofluorescence…

Optics · Physics 2024-08-05 Yayin Tan , Xiaolu Wang , Feng Xu , Xinhao Hu , Yuan Lin , Bo Gao , Zhiqin Chu

The widespread use of nanodiamond as a biomedical platform for drug-delivery, imaging, and sub-cellular tracking applications stems from their non-toxicity and unique quantum mechanical properties. Here, we extend this functionality to the…

Mesoscale and Nanoscale Physics · Physics 2016-06-23 Ewa Rej , Torsten Gaebel , David E. J. Waddington , David J. Reilly

The confluence of quantum physics and biology is driving a new generation of quantum-based sensing and imaging technology capable of harnessing the power of quantum effects to provide tools to understand the fundamental processes of life.…

Quantum Physics · Physics 2015-06-05 D. Maclaurin , L. T. Hall , A. M. Martin , L. C. L. Hollenberg