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Cells in an epithelial monolayer coordinate motion with their neighbors giving rise to collectively moving packs of sizes spanning multiple cell diameters. The physical mechanism controlling the pack size, however, remains unclear. A…

Biological Physics · Physics 2021-07-21 Aashrith Saraswathibhatla , Silke Henkes , Emmett E. Galles , Rastko Sknepnek , Jacob Notbohm

Actin is one of the most studied cytoskeleton proteins showing a very rich span of structures. It can self-assemble actively into dynamical structures that govern the mechanical properties of the cell, its motility and its division.…

Soft Condensed Matter · Physics 2020-03-17 Maayan Levin , Raya Sorkin , David Pine , Rony Granek , Anne Bernheim-Groswasser , Yael Roichman

Force fluctuations exhibited in focal adhesions (FAs) that connect a cell to its extracellular environment, point to the complex role of the underlying machinery that controls cell migration. To elucidate the explicit role of myosin motors…

Biological Physics · Physics 2022-03-29 Debsuvra Ghosh , Subhadip Ghosh , Abhishek Chaudhuri

There is compelling evidence that substrate stiffness affects cell adhesion as well as cytoskeleton organization and contractile activity. This work was designed to study the cytoskeletal contractile activity of cells plated on microposts…

Cell Behavior · Quantitative Biology 2019-09-13 Ian Manifacier , Kevin Beussman , Sangyoon Han , Nathan Sniadecki , Imad About , Jean-Louis Milan

The alignment of cell-cell junctions and associated cortical actomyosin across multiple cells to form supracellular cables in an epithelium is an example of the long range tissue organization that drives morphogenesis. Here we demonstrate…

Tissues and Organs · Quantitative Biology 2018-09-13 Meryl A Spencer , Jesus Lopez-Gay , Hayden Nunley , Yohanns Bellaïche , David K Lubensky

The adaptive and surprising emergent properties of biological materials self-assembled in far-from-equilibrium environments serve as an inspiration for efforts to design nanomaterials and their properties. In particular, controlling the…

Statistical Mechanics · Physics 2023-07-07 Shriram Chennakesavalu , Sreekanth K. Manikandan , Frank Hu , Grant M. Rotskoff

In this communication, we propose a model to study the non-equilibrium process by which actin stress fibers develop force in contractile cells. The emphasis here is on the non-equilibrium thermodynamics, which is necessary to address the…

Subcellular Processes · Quantitative Biology 2014-04-25 Mirko Maraldi , Krishna Garikipati

We present a combined numerical and analytical study of pattern formation in an active system where particles align, possess a density-dependent motility, and are subject to a logistic reaction. This is a model for suspensions of…

Soft Condensed Matter · Physics 2015-06-16 X. Yang , D. Marenduzzo , M. C. Marchetti

We apply a recently developed model of cytoskeletal force generation to study a cell intrinsic contractility, as well as its response to external loading. The model is based on a non-equilibrium thermodynamic treatment of the…

Subcellular Processes · Quantitative Biology 2015-06-19 Mirko Maraldi , Clara Valero , Krishna Garikipati

Motor-proteins are responsible for transport inside cells. Harnessing their activity is key towards developing new nano-technologies, or functional biomaterials. Cytoskeleton-like networks, recently tailored in vitro, result from the…

Soft Condensed Matter · Physics 2016-06-21 Pau Guillamat , Jordi Ignés-Mullol , Francesc Sagués

The mammalian cell membrane is embedded with biomolecular condensates of protein and lipid clusters, which interact with an underlying viscoelastic cytoskeleton network to organize the cell surface and mechanically interact with the…

Soft Condensed Matter · Physics 2024-10-23 Daniel P. Arnold , Sho C. Takatori

Collective behaviors in cellular systems are regulated not only by biochemical signalling pathways but also by intercellular mechanical forces, whose quantification in contractile monolayers remains poorly understood. Here, by integrating…

Quantitative Methods · Quantitative Biology 2025-10-30 Yucheng Huo , Kexin Guo , Massimo Paradiso , K. Jimmy Hsia

Active materials are capable of converting free energy into directional motion, giving rise to striking dynamical phenomena. Developing a general understanding of their structure in relation to the underlying non-equilibrium physics would…

Soft Condensed Matter · Physics 2018-10-17 Nitin Kumar , Rui Zhang , Juan J. de Pablo , Margaret L. Gardel

The actin cytoskeleton is remarkably adaptable and multifunctional. It often organizes into nematic bundles such as contractile rings or stress fibers. However, how a uniform and isotropic actin gel self-organizes into dense nematic bundles…

Subcellular protein complexes and organelles exhibit diverse dynamic behaviors that reflect the mechanical constraints and organization of the intracellular environment. Although some structures follow classical Brownian motion, many…

A motile cell, when stimulated, shows a dramatic increase in the activity of its membrane, manifested by the appearance of dynamic membrane structures such as lamellipodia, filopodia and membrane ruffles. The external stimulus turns on…

Soft Condensed Matter · Physics 2009-11-11 Nir Gov , Ajay Gopinathan

The actin cytoskeleton is a key component in the machinery of eukaryotic cells, and it selfassembles out of equilibrium into a wide variety of biologically crucial structures. While the molecular mechanisms involved are well characterized,…

Soft Condensed Matter · Physics 2017-01-04 Giulia Foffano , Nicolas Levernier , Martin Lenz

Biological actuators -- from myosin motors to muscles -- follow Hill's model where a dimensionless parameter $\alpha$ captures the nonlinear coupling between contraction rate and force generation. Our prior work identified a characteristic…

Adaptation and Self-Organizing Systems · Physics 2026-03-19 Jake McGrath , Colin Johnson , José Alvarado

Current understanding of how contractility emerges in disordered actomyosin networks of non-muscle cells is still largely based on the intuition derived from earlier works on muscle contractility. This view, however, largely overlooks the…

Biological Physics · Physics 2018-04-11 James E. Komianos , Garegin A. Papoian

In biological systems, active agents such as actomyosin and cells move and interact on curved surfaces, exhibiting diverse phenomena. These observations have motivated studies of how curvature shapes their collective behavior. Here, using a…

Soft Condensed Matter · Physics 2025-12-19 Tatsuo Shibata