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In the simplest view of transcriptional regulation, the expression of a gene is turned on or off by changes in the concentration of a transcription factor (TF). We use recent data on noise levels in gene expression to show that it should be…

Molecular Networks · Quantitative Biology 2013-08-01 Gasper Tkacik , Curtis G Callan , William Bialek

In order to survive, reproduce and (in multicellular organisms) differentiate, cells must control the concentrations of the myriad different proteins that are encoded in the genome. The precision of this control is limited by the inevitable…

Molecular Networks · Quantitative Biology 2013-08-01 Gasper Tkacik , Aleksandra M. Walczak , William Bialek

Changes in a cell's external or internal conditions are usually reflected in the concentrations of the relevant transcription factors. These proteins in turn modulate the expression levels of the genes under their control and sometimes need…

Molecular Networks · Quantitative Biology 2013-08-01 Gasper Tkacik , Curtis G Callan , William Bialek

A recurring motif in gene regulatory networks is transcription factors (TFs) that regulate each other, and then bind to overlapping sites on DNA, where they interact and synergistically control transcription of a target gene. Here, we…

Molecular Networks · Quantitative Biology 2013-03-07 Sahand Hormoz

Living cells must control the reading out or "expression" of information encoded in their genomes, and this regulation often is mediated by transcription factors--proteins that bind to DNA and either enhance or repress the expression of…

Molecular Networks · Quantitative Biology 2013-08-01 Gašper Tkačik , Aleksandra M Walczak , William Bialek

Central to the functioning of a living cell is its ability to control the readout or expression of information encoded in the genome. In many cases, a single transcription factor protein activates or represses the expression of many genes.…

Molecular Networks · Quantitative Biology 2013-05-29 Aleksandra M. Walczak , Gapser Tkacik , William Bialek

Cellular decision making is based on regulatory circuits that associate signal thresholds to specific physiological actions. This transmission of information is subjected to molecular noise what can decrease its fidelity. Here, we show…

Molecular Networks · Quantitative Biology 2017-02-08 Guillermo Rodrigo , Juan F. Poyatos

Genetic regulatory circuits universally cope with different sources of noise that limit their ability to coordinate input and output signals. In many cases, optimal regulatory performance can be thought to correspond to configurations of…

Molecular Networks · Quantitative Biology 2018-02-19 Andrea Crisanti , Andrea De Martino , Jonathan Fiorentino

We typically think of cells as responding to external signals independently by regulating their gene expression levels, yet they often locally exchange information and coordinate. Can such spatial coupling be of benefit for conveying…

Molecular Networks · Quantitative Biology 2015-01-19 Thomas R. Sokolowski , Gašper Tkačik

Gene expression is inherently noisy as many steps in the read-out of the genetic information are stochastic. To disentangle the effect of different sources of stochasticity in such systems, we consider various models that describe some…

Molecular Networks · Quantitative Biology 2015-06-05 Rahul Marathe , David Gomez , Stefan Klumpp

Homeostasis of protein concentrations in cells is crucial for their proper functioning, and this requires concentrations (at their steady-state levels) to be stable to fluctuations. Since gene expression is regulated by proteins such as…

Biological Physics · Physics 2020-11-10 Yipei Guo , Ariel Amir

Intracellular transmission of information via chemical and transcriptional networks is thwarted by a physical limitation: the finite copy number of the constituent chemical species introduces unavoidable intrinsic noise. Here we provide a…

Molecular Networks · Quantitative Biology 2010-08-27 Andrew Mugler , Aleksandra M. Walczak , Chris H. Wiggins

The processes of gene expression are inherently stochastic, even for essential genes required for growth. How does the cell maximize fitness in light of noise? To answer this question, we build a mathematical model to explore the trade-off…

Molecular Networks · Quantitative Biology 2024-11-13 Teresa W. Lo , Han James Choi , Dean Huang , Paul A. Wiggins

A crucial step in the regulation of gene expression is binding of transcription factor (TF) proteins to regulatory sites along the DNA. But transcription factors act at nanomolar concentrations, and noise due to random arrival of these…

Molecular Networks · Quantitative Biology 2016-02-10 Thomas R. Sokolowski , Aleksandra M. Walczak , William Bialek , Gašper Tkačik

In vertebrates, olfactory sensory neurons choose only one olfactory receptor to produce out of ~2000 possibilities. The mechanism for how this singular receptor expression occurs is unknown. Here we propose a mechanism that can…

Molecular Networks · Quantitative Biology 2012-01-16 Brian E. Kolterman , Ivan Iossifov , Alexei A. Koulakov

The regulation of cellular function is often controlled at the level of gene transcription. Such genetic regulation usually consists of interacting networks, whereby gene products from a single network can act to control their own…

Biological Physics · Physics 2009-11-06 Jeff Hasty , Joel Pradines , Milos Dolnik , J. J. Collins

We study by simulation the effect of the diffusive motion of repressor molecules on the noise in mRNA and protein levels in the case of a repressed gene. We find that spatial fluctuations due to diffusion can drastically enhance the noise…

Molecular Networks · Quantitative Biology 2007-05-23 Jeroen S. van Zon , Marco J. Morelli , Sorin Tanase-Nicola , Pieter Rein ten Wolde

In many organisms the expression levels of each gene are controlled by the activation levels of known "Transcription Factors" (TF). A problem of considerable interest is that of estimating the "Transcription Regulation Networks" (TRN)…

Applications · Statistics 2010-11-09 Gareth M. James , Chiara Sabatti , Nengfeng Zhou , Ji Zhu

Even under constant external conditions, the expression levels of genes fluctuate. Much emphasis has been placed on the components of this noise that are due to randomness in transcription and translation; here we analyze the role of noise…

Molecular Networks · Quantitative Biology 2013-08-01 Gasper Tkacik , Thomas Gregor , William Bialek

Gene expression is a fundamental process in a living system. The small RNAs (sRNAs) is widely observed as a global regulator in gene expression. The inherent nonlinearity in this regulatory process together with the bursty production of…

Molecular Networks · Quantitative Biology 2021-10-12 Shigang Qiu , Tao Jia
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