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Optical microscopy has a diffraction limited resolution of about 250 nm. Fluorescence methods (e.g. PALM, STORM, STED) beat this, but they are still limited to 10 s of nm, and the images are an indirect pointillist representation of only…

Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization…

Many essential cellular functions depend on the viscoelastic properties of the cytoplasm. While techniques such as optical tweezers and atomic force microscopy can measure these properties, their reliance on localized probes prevents…

Image Phase Alignment Super-Sampling (ImPASS) is a computational imaging algorithm for converting a sequence of displaced low-resolution images into a single high-resolution image. The method consists of a unique combination of Phase…

Optics · Physics 2025-09-01 James N. Caron

Deep-ultraviolet (DUV) microscopy can provide label-free biochemical contrast by exploiting the intrinsic absorption of nucleic acids, proteins and lipids, offering chemically specific morphological information that complements structural…

We present a dual color laser scanning endomicroscope capable of fluorescence lifetime endomicroscopy at one frame per second (FPS). The scanning system uses a coherent imaging fiber with 30,000 cores. High-speed lifetime imaging is…

Fourier ptychography microscopy (FPM), sharing its roots with synthetic aperture technique and phase retrieval method, is a recently developed computational microscopic super-resolution technique. By turning on the light-emitting diode…

Optics · Physics 2021-05-07 G. Zhou , S. Zhang , C. Zheng , T. Li , Y. Hu , Q. Hao

Label-free optical microscopy through absorption or scattering spectroscopy provides fundamental insights across biology and materials science, yet its sensitivity remains fundamentally limited by photon shot noise. While recent…

Endoscopy is the most widely used medical technique for cancer and polyp detection inside hollow organs. However, images acquired by an endoscope are frequently affected by illumination artefacts due to the enlightenment source orientation.…

Image and Video Processing · Electrical Eng. & Systems 2022-07-07 Axel Garcia-Vega , Ricardo Espinosa , Gilberto Ochoa-Ruiz , Thomas Bazin , Luis Eduardo Falcon-Morales , Dominique Lamarque , Christian Daul

Endoscopes featuring a miniaturized design have significantly enhanced operational flexibility, portability, and diagnostic capability while substantially reducing the invasiveness of medical procedures. Recently, single-use endoscopes…

Image and Video Processing · Electrical Eng. & Systems 2025-06-19 Yu Xing , Shishi Huang , Meng Lv , Guo Chen , Huailiang Wang , Lingzhi Sui

Automated label-free quantitative imaging of biological samples can greatly benefit high throughput diseases diagnosis. Digital holographic microscopy (DHM) is a powerful quantitative label-free imaging tool that retrieves structural…

Endoscopy has been an indispensible tool in medical diagnostics, and yet the demands for reduced unit diameter and enhanced spatial resolution have steadily been growing for the accurate investigation of distal sites with minimal side…

We present an ultra-fast, precise, parameter-free method, which we term Deep-STORM, for obtaining super-resolution images from stochastically-blinking emitters, such as fluorescent molecules used for localization microscopy. Deep-STORM uses…

Optics · Physics 2018-05-03 Elias Nehme , Lucien E. Weiss , Tomer Michaeli , Yoav Shechtman

In this work, we focus on boosting the feature extraction to improve the performance of Structure-from-Motion (SfM) in endoscopy videos. We present SuperPoint-E, a new local feature extraction method that, using our proposed Tracking…

Computer Vision and Pattern Recognition · Computer Science 2026-02-05 O. Leon Barbed , José M. M. Montiel , Pascal Fua , Ana C. Murillo

Correlative microscopy is a powerful technique that combines the advantages of multiple imaging modalities to achieve a comprehensive understanding of investigated samples. For example, fluorescence microscopy provides unique functional…

We have developed a hyperspectral imaging scheme that involves a combination of dual-comb spectroscopy and Hadamard-transform-based single-pixel imaging. The scheme enables us to obtain 12,000 hyperspectral images of amplitude and phase at…

Super-resolution microscopes (such as STED) illuminate samples with a tiny spot, and achieve very high resolution. But structures smaller than the spot cannot be resolved in this way. Therefore, we propose a technique to solve this problem.…

Image and Video Processing · Electrical Eng. & Systems 2019-11-20 Yaohua Xie

Recovering both amplitude and phase information from a system is a fundamental goal of optical imaging. At the same time, it is crucial to operate at low photon doses to avoid altering the sample, particularly in biological applications.…

Quantum Physics · Physics 2026-01-15 Alberto Paniate , Giuseppe Ortolano , Sarika Soman , Marco Genovese , Ivano Ruo-Berchera

Automatic medical image segmentation plays a crucial role in computer aided diagnosis. However, fully supervised learning approaches often require extensive and labor-intensive annotation efforts. To address this challenge, weakly…

Computer Vision and Pattern Recognition · Computer Science 2025-03-20 Lei Shi , Xi Fang , Naiyu Wang , Junxing Zhang

A fundamental challenge in endoscopy is how to fabricate a small fiber-optic probe that can achieve comparable function to probes with large, complicated optics (e.g., high resolution and extended depth of focus). To achieve high resolution…