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Hydrated proteins undergo a transition in the deeply supercooled regime, which is attributed to rapid changes in hydration water and protein structural dynamics. Here, we investigate the nanoscale stress relaxation in hydrated lysozyme…

Employing X-ray photon correlation spectroscopy we measure the kinetics and dynamics of a pressure-induced liquid-liquid phase separation (LLPS) in a water-lysozyme solution. Scattering invariants and kinetic information provide evidence…

Soft Condensed Matter · Physics 2021-07-13 M. Moron , A. Al-Masoodi , C. Lovato , M. Reiser , L. Randolph , G. Surmeier , J. Bolle , F. Westermeier , M. Sprung , R. Winter , M. Paulus , C. Gutt

We present a pressure dependence study of the dynamics of lysozyme protein powder immersed in deuterated $\alpha$,$\alpha$-trehalose environment via quasi-elastic neutron scattering (QENS). The goal is to assess the baro-protective benefits…

Soft Condensed Matter · Physics 2015-06-22 S. O. Diallo , Q. Zhang , H. O'Neill , E. Mamontov

Relaxation dynamics, as a key to understand glass formation and glassy properties, remains an elusive and challenging issue in condensed matter physics. In this work, in situ high-pressure synchrotron high-energy x-ray photon correlation…

A new experimental setup combining X-Ray Photon Correlation Spectroscopy (XPCS) in the hard x-ray regime and a high-pressure sample environment is developed to monitor the pressure dependence of the internal motion of complex systems down…

Recently we have shown experimental evidence for a fragile-to-strong dynamic crossover (FSC) phenomenon in hydration water around a globular protein (lysozyme) at ambient pressure. In this letter we show that when applying pressure to the…

CrAs was firstly observed possessing the bulk superconductivity (Tc~2 K) under high pressure (0.8 GPa) in the very recent work (Wei Wu, et al. Nature Communications 5, 5508 (2014)). To explore the correlation between the structure and the…

Strongly Correlated Electrons · Physics 2014-10-27 Zhenhai Yu , Wei Wu , Jinggeng Zhao , Chunyu Li , Jinguang Cheng , Lin Wang , Jianlin Luo

Studying protein interactions at low temperatures has important implications for optimizing cryostorage processes of biological tissue, food, and protein-based drugs. One of the major challenges is related to the formation of ice…

The effects of static electric field on the dynamics of lysozyme and its hydration water have been investigated by means of incoherent quasi-elastic neutron scattering (QENS). Measurements were performed on lysozyme samples, hydrated…

Soft Condensed Matter · Physics 2013-12-09 P. M. Favi , Q. Zhang , H. O'Neill , E. Mamontov , S. O. Diallo

We have studied the effect of high hydrostatic pressure and temperature on the steady state fluorescence anisotropy of Green Fluorescent Protein (GFP). We find that the fluorescence anisotropy of GFP at a constant temperature decreases with…

Biomolecules · Quantitative Biology 2022-02-16 Harpreet Kaur , Khanh Nguyen , Pradeep Kumar

In the present work, we provide a dielectric study on two differently concentrated aqueous lysozyme solutions in the frequency range from 1 MHz to 40 GHz and for temperatures from 275 to 330 K. We analyze the three dispersion regions,…

Soft Condensed Matter · Physics 2012-03-29 M. Wolf , R. Gulich , P. Lunkenheimer , A. Loidl

We have measured plasmon energies in Na under high pressure up to 43 GPa using inelastic x-ray scattering (IXS). The momentum-resolved results show clear deviations, growing with increasing pressure, from the predictions for a nearly-free…

Materials Science · Physics 2011-10-11 I. Loa , K. Syassen , G. Monaco , G. Vanko , M. Krisch , M. Hanfland

Simple binary oxide glasses can exhibit a compression behavior distinct from that of their crystalline counterparts. In this study, we employed high-pressure X-ray absorption spectroscopy coupled to the diamond anvil cell to investigate in…

The effects of high pressure on the crystal structure of orthorhombic (Pnma) perovskite type cerium scandate have been studied in situ under high pressure by means of synchrotron x-ray powder diffraction, using a diamond anvil cell. We have…

We report high pressure X-ray diffraction and a detailed systematic Raman measurements on $ReS_2$ sample, which is mechanically exfoliated from a single crystal. A few new Bragg peaks are observed to emerge above 6 GPa indicating a…

Materials Science · Physics 2020-05-20 Pinku Saha , Bishnupada Ghosh , Aritra Mazumder , Konstantin Glazyrin , Goutam Dev Mukherjee

We study theoretically the thermodynamics, over a broad temperature range (5 C to 125 C), related to hydrated water upon protein unfolding. The hydration effect is modeled as interacting dipoles in an external field, mimicking the influence…

Condensed Matter · Physics 2009-11-07 Audun Bakk , Johan S. Hoye , Alex Hansen

Measurements of chain conformation in proteins/polyelectrolytes complexes (lysozyme and PSSNa) show that the crossover observed between an open structure -a chain network crosslinked by the proteins, and a globular one - dense globules of ~…

Soft Condensed Matter · Physics 2009-03-31 Jérémie Gummel , Fabrice Cousin , François Boué

Water and glycerol are well-known to facilitate the structural relaxation of amorphous protein matrices. However, several studies evidenced that they may also limit fast ($\sim$ pico-nanosecond, ps-ns) and small-amplitude ($\sim$ \AA )…

Soft Condensed Matter · Physics 2017-10-03 Adrien Lerbret , Frédéric Affouard

X-ray diffraction (XRD) experiments have been carried out on quartz-like GaPO4 at high pressure and room temperature. A transition to a high pressure disordered crystalline form occurs at 13.5 GPa. Slight heating using a YAG infrared laser…

Condensed Matter · Physics 2009-10-31 James Badro , Jean-Paul Itie , Alain Polian

At low temperatures proteins exist in a glassy state, a state which has no conformational flexibility and shows no biological functions. In a hydrated protein, at and above 220 K, this flexibility is restored and the protein is able to…

Soft Condensed Matter · Physics 2009-11-11 Sow-Hsin Chen , Li Liu , Emiliano Fratini , Piero Baglioni , Antonio Faraone , Eugene Mamontov
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