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Recent developments in fluorescence microscopy allow capturing high-resolution 3D images over time for living model organisms. To be able to image even large specimens, techniques like multi-view light-sheet imaging record different…

Image and Video Processing · Electrical Eng. & Systems 2021-08-29 Canyu Yang , Dennis Eschweiler , Johannes Stegmaier

Fluorescence lifetime imaging microscopy (FLIM) is an important technique to understand the chemical micro-environment in cells and tissues since it provides additional contrast compared to conventional fluorescence imaging. When two…

Image and Video Processing · Electrical Eng. & Systems 2022-01-19 Varun Mannam , Xiaotong Yuan , Scott Howard

Deformable convolution, originally proposed for the adaptation to geometric variations of objects, has recently shown compelling performance in aligning multiple frames and is increasingly adopted for video super-resolution. Despite its…

Computer Vision and Pattern Recognition · Computer Science 2020-09-16 Kelvin C. K. Chan , Xintao Wang , Ke Yu , Chao Dong , Chen Change Loy

Optical microscopy is an essential tool in biology and medicine. Imaging thin, yet non-flat objects in a single shot (without relying on more sophisticated sectioning setups) remains challenging as the shallow depth of field that comes with…

Image and Video Processing · Electrical Eng. & Systems 2020-10-14 Adrian Shajkofci , Michael Liebling

Due to limited size and imperfect of the optical components in a spectrometer, aberration has inevitably been brought into two-dimensional multi-fiber spectrum image in LAMOST, which leads to obvious spacial variation of the point spread…

Instrumentation and Methods for Astrophysics · Physics 2020-10-28 Jiali Xu , Qian Yin , Ping Guo , Xin Zheng

Images from adaptive optics systems are generally affected by significant distortions of the point spread function (PSF) across the field of view, depending on the position of natural and artificial guide stars. Image reduction techniques…

Instrumentation and Methods for Astrophysics · Physics 2015-06-24 Andrea La Camera , Laura Schreiber , Emiliano Diolaiti , Patrizia Boccacci , Mario Bertero , Michele Bellazzini , Paolo Ciliegi

A method for spatial deconvolution of spectra is presented. It follows the same fundamental principles as the ``MCS image deconvolution algorithm'' (Magain, Courbin, Sohy, 1998) and uses information contained in the spectrum of a reference…

Astrophysics · Physics 2009-10-31 F. Courbin , P. Magain , M. Kirkove , S. Sohy

We describe a simple and robust approach for characterizing the shift-variant pupil aberrations of wide field-of-view microscopy systems. We derive the location-dependent pupil transfer functions by first capturing multiple intensity images…

Optics · Physics 2016-12-21 Guoan Zheng , Xiaoze Ou , Roarke Horstmeyer , Changhuei Yang

The study of astronomical phenomena through ground-based observations is always challenged by the distorting effects of Earth's atmosphere. Traditional methods of post-facto image correction, essential for correcting these distortions,…

Instrumentation and Methods for Astrophysics · Physics 2024-08-14 A. Asensio Ramos

In multi-photon microscopy (MPM), a recent in-vivo fluorescence microscopy system, the task of image restoration can be decomposed into two interlinked inverse problems: firstly, the characterization of the Point Spread Function (PSF) and…

Image and Video Processing · Electrical Eng. & Systems 2024-11-08 Julien Ajdenbaum , Emilie Chouzenoux , Claire Lefort , Ségolène Martin , Jean-Christophe Pesquet

Spatial resolution is one of the most important specifications of an imaging system. Recent results in quantum parameter estimation theory reveal that an arbitrarily small distance between two incoherent point sources can always be…

This paper demonstrates a practical method that can correct spatial varying blur from a set of images of the same object. The algorithm jointly estimates the object and local point spread functions~(PSF). The method prioritizes sections…

Image and Video Processing · Electrical Eng. & Systems 2020-11-04 Wouter van de Ketterij , Oleg Soloviev , Michel Verhaegen

In light-sheet microscopy, overall image content and resolution are improved by acquiring and fusing multiple views of the sample from different directions. State-of-the-art multi-view (MV) deconvolution employs the point spread functions…

Quantitative Methods · Quantitative Biology 2015-03-30 Benjamin Schmid , Jan Huisken

We generalize Richardson-Lucy (RL) deblurring to 4-D light fields by replacing the convolution steps with light field rendering of motion blur. The method deals correctly with blur caused by 6-degree-of-freedom camera motion in complex 3-D…

Computer Vision and Pattern Recognition · Computer Science 2017-05-22 Donald G. Dansereau , Anders Eriksson , Jürgen Leitner

A non iterative direct blind deconvolution procedure, previously used successfully to sharpen Hubble Space Telescope imagery, is now found useful in sharpening nanoscale scanning electron microscope (SEM) and helium ion microscope (HIM)…

Instrumentation and Detectors · Physics 2025-02-28 Alfred S. Carasso , Andras E. Vladar

In medical image segmentation, particularly in UNet-like architectures, upsampling is primarily used to transform smaller feature maps into larger ones, enabling feature fusion between encoder and decoder features and supporting multi-scale…

Computer Vision and Pattern Recognition · Computer Science 2026-01-27 Chengkun Sun , Jinqian Pan , Renjie Liang , Zhengkang Fan , Xin Miao , Jiang Bian , Jie Xu

Fluorescence microscopy is widely used for the study of biological specimens. Deconvolution can significantly improve the resolution and contrast of images produced using fluorescence microscopy; in particular, Bayesian-based methods have…

Methodology · Statistics 2015-02-04 Alexander Wong , Xiao Yu Wang , Maud Gorbet

We present a blind multiframe image-deconvolution method based on robust statistics. The usual shortcomings of iterative optimization of the likelihood function are alleviated by minimizing the M-scale of the residuals, which achieves more…

Instrumentation and Methods for Astrophysics · Physics 2017-11-09 Matthias Lee , Tamas Budavari , Richard White , Charles Gulian

A common setting for scientific inference is the ability to sample from a high-fidelity forward model (simulation) without having an explicit probability density of the data. We propose a simulation-based maximum likelihood deconvolution…

Super-resolution techniques overcome the diffraction-limit and get very high resolutions. A category of these techniques, e.g., STED achieves this by creating an illumination spot smaller than the Airy Disk. As a result, points are…

Image and Video Processing · Electrical Eng. & Systems 2019-11-20 Yaohua Xie